1. CHROMATOGRAPHY 1Biochemistry of Medics

2. Chromatography 2Biochemistry of Medics

3. Chromatography 3Biochemistry of Medics

4. Chromatography- Principle 4Biochemistry of Medics

5. Chromatography- Classification 5Biochemistry of Medics

6. Chromatography- Classification(Contd.) 6Biochemistry of Medics

7. Partition Chromatography 7Biochemistry of Medics

8. Paper chromatography 8Biochemistry of Medics

9. Paper chromatography 9Biochemistry of Medics

10. Paper chromatography- Procedure 10Biochemistry of Medics

11. Paper chromatography- Procedure 11Biochemistry of Medics

12. Ascending and Descending Paper Chromatography 12Biochemistry of Medics

13. Paper Chromatography -Analysis 13Biochemistry of Medics

14. R ƒ value 14Biochemistry of Medics

15. Two dimensional chromatography 15Biochemistry of Medics

16. Significance of Paper chromatography 16Biochemistry of Medics

17. Thin layer chromatography (TLC) 17Biochemistry of Medics

18. TLC- Procedure 18Biochemistry of Medics

19. TLC -Overview 19Biochemistry of Medics

20. Significance of TLC 20Biochemistry of Medics

21. Advantages of TLC over paper chromatography 21Biochemistry of Medics

22. 2)Adsorption chromatography 22Biochemistry of Medics

23. 2)Adsorption chromatography- Elution 23Biochemistry of Medics

24. Adsorption chromatography The substance loosely adsorbed to the stationary medium comes in the earlier fraction. 24Biochemistry of Medics

25. Ion – Exchange chromatography 25Biochemistry of Medics

26. Ion – Exchange chromatography 26Biochemistry of Medics

27. Ion – Exchange chromatography- Procedure 27Biochemistry of Medics

28. Ion – Exchange chromatography- Procedure (Contd.) 28Biochemistry of Medics

29. Types of ion exchanges resins 29Biochemistry of Medics

30. Ion – Exchange chromatography- An Overview 30Biochemistry of Medics

31. 4) Gel filtration chromatography or Molecular Sieve chromatography 31Biochemistry of Medics

32. Gel filtration chromatography or Molecular Sieve chromatography 32Biochemistry of Medics

33. Gel filtration chromatography-An Overview Larger particles come out first, while smaller particles come in later fractions 33Biochemistry of Medics

34. 5) Affinity chromatography 34Biochemistry of Medics

35. Affinity chromatography- Significance 35Biochemistry of Medics

36. Affinity chromatography- Significance By using antibodies, antigens can be easily separated. Conversely, antibodies can be purified by passing through a column containing the antigen. Specific Antibody binds to a specific antigen attached to the stationary medium, the remaining antibodies come out in earlier fractions. 36 Biochemistry of Medics

37. 6) High performance liquid chromatography- 37Biochemistry of Medics

38. High performance liquid chromatography- 38Biochemistry of Medics

39. High performance liquid chromatography- Significance 39Biochemistry of Medics

40. High performance liquid chromatography- Apparatus 40Biochemistry of Medics

41. 6) Gas liquid chromatography- 41Biochemistry of Medics

42. Gas liquid chromatography- 42Biochemistry of Medics

43. Gas liquid chromatography-Apparatus 43Biochemistry of Medics

44. Summary 44Biochemistry of Medics