1. Microscopes Seeing Small!!!

2. Microscope – (small/look) a device used to observe and examine things too small to see by eye a device used to observe and examine things too small to see by eye

3. Magnification The increase in apparent size of an object The increase in apparent size of an object ex dust mite

4. Resolution How clearly details are shown when magnified How clearly details are shown when magnified Which photo has the best resolution? Which photo has the best resolution?

5. Microscope types 1) Light Microscopes – use visible light 1) Light Microscopes – use visible light 2) Electron Microscopes – use electrons 2) Electron Microscopes – use electrons Each of these have sub types Each of these have sub types

6. Compound Microscope – uses two different type lenses uses two different type lenses and light source. and light source. Views things that are thin Views things that are thin enough to see through. Magnifies up to 1000 X Magnifies up to 1000 X

7. Stereo Microscope Uses 2 eyepieces and light source. Uses 2 eyepieces and light source. Used for larger objects and views in 3-D. Used for larger objects and views in 3-D.

8. Leeuwenhoek’s microscope Saw first living microorganisms Saw first living microorganisms

9. Electron Microscopes MUCH more complex MUCH more complex

10. Scanning electron microscope Uses beam of electrons to magnify over 100,000 X. Uses beam of electrons to magnify over 100,000 X. Bounces electrons off specimen. Bounces electrons off specimen.

11. Transmission electron microscope Electron beam goes through thin specimens Electron beam goes through thin specimens Up to 1,000,000 times Up to 1,000,000 times

12. Transmission Scanning

13. What is it? Fly eye Fly eye

14. What is it Fire ant Fire ant

15. What is it? Tobacco seed Tobacco seed

16. What is it? Fly foot Fly foot

17. What is it? Pollen Pollen

18. Lenses Ocular (eyepiece) – The lens you look through. Ocular (eyepiece) – The lens you look through. Objective – Aims at specimen. Usually changeable. Objective – Aims at specimen. Usually changeable.

19. Magnification power Total Power = Ocular X Objective Total Power = Ocular X Objective Ex. Ocular = 10X Objective = 40X Ex. Ocular = 10X Objective = 40X 10X times 40X = 400X Total magnification 400X Total magnification