1. Bacterial Identification Using Confocal Raman Spectroscopy Brian Cox, Kevin Smith Advisors: Dr. Mahadevan-Jansen Chad Lieber

2. Overview Construct the Confocal Raman Spectroscopy system Test accuracy from commercially obtained samples Create library from in vitro samples

3. Background Raman Spectrosopy –Uses near IR irradiation (~785nm laser) –Based on vibrational bond energies of molecules –Inelastic scattering technique –Low water signal is good for biological samples Confocal –High precision focus –Reduced noise

4. Background Otitis Media –Account for more than 21 million doctors' visits each year –3/4 of all children have at least one ear infection before their third birthday –Most commonly strike kids between 6 months and 2 years and often follow a cold –Cold virus causes congestion in the eustachian tube, which leads from the throat to the ear, and results in a buildup of fluid in the middle ear

5. Reasoning Otitis media is second most common affliction in children Most common cause of hearing loss in children Extremely rapid diagnosis Non-invasive Current treatment methods are non-specific

6. Past Work Researched Raman Spectroscopy Finalized design Ordered initial hardware

7. Current Design

8. Current Work Construction of the device Determining additional components

9. Current Problems Fiber Coupling (300um multimode to 6um monomode) –Spot size vs. incidence angle –Focal lengths of suitable lenses are too long –GRIN lenses Mirror translation must be locked into place, but easily moved.

10. Future Work Order remaining components Complete construction of device Testing device