1. Study Population In 2008-09, 1,154 Mexican origin youth (aged 13-16 years) indicated which of 50 movies (randomly selected from a pool of 250 popular movies released from 2003-07) they had seen and provided data on alcohol-related risk factors (e.g. family environment, anxiety). In 2010-11, 1,002 participants (87%) completed follow-up. Data Collection All survey data were collected in the participants’ homes using a hand held personal digital assistant to ensure confidentiality. Of the 250 movies we examined,150 were content analyzed. Therefore, the number of movies include in the current analysis for each participant varied between 26 and 32. Data were collected in Spanish or English and were self-reported. N=989 participants had completed data on the major study variables. Description of the Measures Main outcome: Alcohol use was assessed using the YRBSS (3) item: “During your life, on how many days have you had at least one drink of alcohol?” Responses of 0 days were coded as “Never,” all other responses were coded as “Ever.” Main variable of interest: Exposure to movies depicting alcohol use was calculated by summing the number of alcohol occurrences in each movie the participant had seen. The resulting variable scaled alcohol time, can be analyzed overall or based on movie ratings (G/PG, PG13, R). Participant characteristics: Gender (male or female), age at baseline (11–13 years old in 2005- 06) as well as child’s country of birth and parents’ country of birth (Mexico or USA). Galya Bigman, 1 James D Sargent, 2 Margaret R Spitz, 3 Anna V Wilkinson 1 1. University of Texas School of Public Health, 2. Dartmouth Medical School, 3. Baylor College of Medicine. Social covariates: No. of friends who drink alcohol: None, 1, >1 No. of household members who drink alcohol: None, 1, >1 Perceived family conflict and cohesion assessed using the Family Life Questionnaire. (4) Each subscale comprises four items (family conflict alpha=0.55 and family cohesion alpha=0.67); response options range between 1 and 4. Individual covariates: Subjective Social Status assessed using the MacArthur Scale for Adolescents. (5) High scores reflect children with good grades, who have lots of friends and who do well in sports. Sensation Seeking Subscale assessed using 26 forced-choice items that form three subscales: Drug & Alcohol Subscale (DAA; alpha=0.72), Social Disinhibition (SD; alpha=0.68) and Thrill & Adventure Seeking (TAS; alpha=0.81). (6) Participants select the choice that best describes what they like or how they feel. Anxiety assessed using Spielberg's trait anxiety scale; includes 20 items (alpha=0.86) and had been validated with Spanish- speaking teenagers in the US. (7) Statistical Analyses Univariable analyses: Chi-square tests were used to determine differences in alcohol use rates (Ever vs. Never) by participant characteristics (Fig 2). T-tests were used to examine differences between mean exposure to movies depicting alcohol use by participant characteristics (Fig 3). Multivariable Analyses: Logistic regression analyses were preformed to determine associations between ever alcohol use and the main variable of interest – exposure to movies depicting alcohol use, controlling for participant characteristics, as well as the social and individual covariates (Table 2). Main Participants with high exposure to movies depicting alcohol use that are rated PG13 & R were more likely to have ever tried alcohol themselves, by 60% and 40% respectively, compared to those with lower exposure. About 60% of our participants were exposed to PG13 and R rated movies. Secondary Participants with one or more family members who drink alcohol are roughly 3 times more likely to have ever tried alcohol compared to those whose family members do not drink alcohol. Participants whose parents were born in Mexico were 55% more likely to have ever tried alcohol compared to participants whose parents were born in the USA. Background Objectives Conclusions Acknowledgements: This study was supported by the National Cancer Institute (R01 CA105203 to MRS and K07 CA126988 to AVW). We thank the Mano a Mano cohort staff for their ongoing work with participant recruitment and follow-up. The Mano a Mano cohort is funded by funds collected pursuant to the Comprehensive Tobacco Settlement of 1998 and appropriated by the 76 th legislature to the University of Texas M.D. Anderson Cancer Center; by the Caroline W. Law Fund for Cancer Prevention; and by the Dan Duncan Family Institute for Cancer Prevention and Risk Assessment. We would like to express our gratitude to the participants of this study and the MATCh research team for their hard work collecting the data. T ABLE 2. Full models of the logistic regression for ever alcohol use stratified by movie rating G/PG, PG13 and R. * Since “No. of friends who drink alcohol” is a potential mediator between alcohol use and movies depicting alcohol use the variable was not included in the multivariable analysis Exposure to movies depicting alcohol use impacts alcohol use among youth. (1-2) Less is known about this association among minority youth, in particular whether it holds and how such an association might relate to established alcohol-related risk factors. F IGURE 3. Mean exposure to movies depicting alcohol use in PG13 rated movies by alcohol use (ever vs. never) for participant characteristics. Results F IGURE 1. % of Ever vs. Never alcohol use by participant characteristics F IGURE 2. Overall distribution of movies ratings each participant reported seeing. Main To examine the association between movies depicting alcohol use and alcohol use among Mexican origin youth Secondary To examine the association between social and individual factors and alcohol use among Mexican origin youth Methods Results T ABLE 1. Descriptive characteristics of study participants * * * * * * * * * p<0.05