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A viral protein inhibits the long range signaling activity of the gene silencing signal by Hui Shan Guo, and Shou Wei Ding EMBO J. Volume 21(3):398-407.

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Presentation on theme: "A viral protein inhibits the long range signaling activity of the gene silencing signal by Hui Shan Guo, and Shou Wei Ding EMBO J. Volume 21(3):398-407."— Presentation transcript:

1 A viral protein inhibits the long range signaling activity of the gene silencing signal by Hui Shan Guo, and Shou Wei Ding EMBO J. Volume 21(3):398-407 February 1, 2002 ©2002 by European Molecular Biology Organization

2 Incomplete suppression of GFP transgene silencing in the infiltrated leaves of the N.benthamiana plants. Hui Shan Guo, and Shou Wei Ding EMBO J. 2002;21:398- 407 ©2002 by European Molecular Biology Organization

3 Differential suppression of local and systemic silencing of the GFP transgene in the N.benthamiana GFP plants with or without Cmv2b expression. Hui Shan Guo, and Shou Wei Ding EMBO J. 2002;21:398- 407 ©2002 by European Molecular Biology Organization

4 Suppression of the intercellular spread of GUS transgene silencing in N.tabacum plants. Hui Shan Guo, and Shou Wei Ding EMBO J. 2002;21:398- 407 ©2002 by European Molecular Biology Organization

5 Diagrams illustrating the grafting and infiltration experiments used to analyze the role of Cmv2b in blocking the signal ‐ mediated spread of PTGS. Small red dots denote the GUS sRNAs, the red area indicates induction of GFP silencing in regions infiltrated w... Hui Shan Guo, and Shou Wei Ding EMBO J. 2002;21:398- 407 ©2002 by European Molecular Biology Organization

6 Inhibition of the signal ‐ mediated spread of PTGS in N.tabacum plants. Hui Shan Guo, and Shou Wei Ding EMBO J. 2002;21:398- 407 ©2002 by European Molecular Biology Organization

7 Inhibition of the signal ‐ mediated spread of PTGS in N.benthamiana plants. Hui Shan Guo, and Shou Wei Ding EMBO J. 2002;21:398- 407 ©2002 by European Molecular Biology Organization

8 Induction of GFP transgene silencing by the translocated silencing signal. Hui Shan Guo, and Shou Wei Ding EMBO J. 2002;21:398- 407 ©2002 by European Molecular Biology Organization

9 Cmv2b expression partially prevented restoration of transgene methylation in N.tabacum plants. Hui Shan Guo, and Shou Wei Ding EMBO J. 2002;21:398- 407 ©2002 by European Molecular Biology Organization


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