1. CISB Research Planning, Years 4 & 5 Aim 1: Targeting malfunctioning biological systems in key diseases Aim 2: Engineering scalable synthetic networks Aim 3: Platform technologies Plan: 1.Each PI to confirm bullet points 2.Each PI create one slide per point from the following slides

2. Aim 1: Targeting malfunctioning biological systems in key diseases 1.1: Cancer [RW, TL] – Mapping miRNA profiles – CombiGEM for cancer Combinatorial drug pairs, Cas9 – Cancer immunotherapy 1.2: Microbiome engineering and pathogens [TL, CV] – Technologies for Malaria: combinatorial regulation / pathway analysis via orthogonal translational regulation and transcriptional regulation (Cas9/TetR) – Microbiome engineering: broadening the range of chasses (bacteriodes), engineering strategies for therapeutic production from bacteroides (sensors & output) – Phage engineering 1.3: Diabetes [RW] – Analog circuit design for artificial tissue homeostasis – hiPS to pancreas differentiation – hiPS reprogramming [DDV]

3. Aim 2: Engineering scalable synthetic networks Cello 2.0 [CV] Information processing via recombinases for state machines [TL] Resource sharing [DDV] Load drivers [DDV] Analysis / systems biology: example is Malaria, and maybe cancer [JN] Metabolic burden [KP] Cas9 transcriptional families [CV / TL / RW]

4. Aim 3: Platform technologies Metafluidics [PC] DNA assembly on a chip [PC] Integrated assembly + analysis on a chip [PC] Cell free tx/tl [PC] (Relationship to MIT Broad Foundry?) [CV]