1. MBV2010 Colloquium, April 14

2. RULES Multiple choice Only one correct answer 30-60 seconds to answer No textbook, computer, mobile phone, please There will be a prize for the group with the most correct answers.

3. Which of the following numbers is the largest? A 4 x 10 -3 B 2 x √ 10 -4 C 0.005 D (0.1) 2 0

4. Which of the following numbers is the largest? 0

5. Which of the following introns contains a guanine-binding pocket? A Group I introns B Group II introns C AU-AC introns D Eukaryotic tRNA introns 1

6. Which of the following introns contains a guanine-binding pocket? 1

7. The carboxy-terminal domain of RNA polymerase II consists of a repeated A heptapeptide (7 repeats) B hexapeptide (6 repeats) C octapeptide (8 repeats) D decapeptide (10 repeats) 2

8. The carboxy-terminal domain of RNA polymerase II consists of a repeated 2 Tyr-Ser-Pro-Thr-Ser-Pro-Ser

9. The human CTD consists of A 27 repeats B 32 repeats C 45 repeats D 52 repeats 3 Tyr-Ser-Pro-Thr-Ser-Pro-Ser

10. The human CTD consists of 3 Tyr-Ser-Pro-Thr-Ser-Pro-Ser

11. The TATA-binding protein (TBP) is part of A Transcription factor IIA B Transcription factor IIB C Transcription factor IID D Transcription factor IIE 4

12. The TATA-binding protein (TBP) is part of 4

13. FACT is a protein complex involved in A Transcription B Replication C Translation D Recombination 5 ?

14. FACT is a protein complex involved in 5 FACT = facilitates chromatin transcription

15. The rho protein mediates A Initiation of transcription B Initiation of replication C Site-specific recombination D Termination of transcription 6

16. The rho protein mediates 6

17. Deamination of cytosine yields A adenine B thymine C uracil D guanine 7

18. Deamination of cytosine yields 7

19. Which of the following proteins plays a role in splice site selection? A snRNPs B SR proteins C Sxl protein D U2 8

20. Which of the following proteins plays a role in splice site selection? 8

21. Amino acids are linked to tRNAs at A the 5’ terminus of tRNAs B the adenine base at the 3’ terminus C the ribose at the 3’ terminus D the phosphate linking the 3’ terminal nucleotides of tRNAs 9

22. Amino acids are linked to tRNAs at 9

23. Which of the following RNAs contains dihydrouridine? A ribosomal RNAs B tRNAs C mRNAs D small nuclear RNAs 10

24. Which of the following RNAs contains dihydrouridine? 10

25. Which of the following RNAs contains pseudouridines? A ribosomal RNA B mRNA C small nuclear RNA D small nucleolar RNA 11

26. Which of the following RNAs contains pseudouridines? 11 Pseudouridinylations are introduced into rRNA by a similar mechanism as methylations.