1. INDIAN INSTITUTE OF TECHNOLOGY ROORKEE Development of 5- fluorouracil loaded hydrogel and study of its anticancer potential Sonal Omer Dr. Bharat Bhushan, Dr.P.Gopinath Dept. of Biotechnology and Centre of Nanotechnology Indian Institute of Technology, Roorkee

2. Methodology Fabricate an semi-IPN hydrogel Easy to make and sterilize Localized therapeutic behavior Fabricate an semi-IPN hydrogel Easy to make and sterilize Localized therapeutic behavior Green method for fabrication of hydrogel Project Objectives

3. Characterization of Semi-IPN Hydrogel (A) (B) Nano-scale surface morphology Internal porous cross-section

4. Cell viability of HCT- 116 cells estimated by MTT assay after 48 hours of treatment. Anti-cancer potential of hydrogel in HCT-116 as an cancer cell model Late apoptotic cells Early apoptotic cells Fluorescence images of Acridine orange and Ethidium bromide stained HCT-116 cells (a)Untreated (b)half IC 50 treated (c)IC 50 treated (d)2 IC 50 treated