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Immune regulatory activity of CD34 + progenitor cells: evidence for a deletion-based mechanism mediated by TNF-α by Hilit Gur, Rita Krauthgamer, Esther.

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Presentation on theme: "Immune regulatory activity of CD34 + progenitor cells: evidence for a deletion-based mechanism mediated by TNF-α by Hilit Gur, Rita Krauthgamer, Esther."— Presentation transcript:

1 Immune regulatory activity of CD34 + progenitor cells: evidence for a deletion-based mechanism mediated by TNF-α by Hilit Gur, Rita Krauthgamer, Esther Bachar-Lustig, Helena Katchman, Rinat Arbel- Goren, Alain Berrebi, Tirza Klein, Arnon Nagler, Antonio Tabilio, Massimo F. Martelli, and Yair Reisner Blood Volume 105(6):2585-2593 March 15, 2005 ©2005 by American Society of Hematology

2 CD34+ cell–mediated suppression of CTL responses is not prevented by addition of anti-CD28 mAb. Hilit Gur et al. Blood 2005;105:2585-2593 ©2005 by American Society of Hematology

3 T cells cultured in the presence of CD34+ cells do not exhibit suppressive activity in a secondary MLR. A 6-day MLR was established in which responder cells (1 × 106/mL) were cultured with irradiated allogeneic stimulator cells (1 × 106/mL) of the CD34+ cel... Hilit Gur et al. Blood 2005;105:2585-2593 ©2005 by American Society of Hematology

4 Cellular levels of pDC1 (GM-CSFRα chain, CD13) versus pDC2 (IL-3Rα chain) induced by the addition of CD34+ cells to the bulk MLR. A 5-day MLR was established in which responder cells (1 × 106/mL) were cocultured with irradiated allogeneic stimulator cells (... Hilit Gur et al. Blood 2005;105:2585-2593 ©2005 by American Society of Hematology

5 CD34+ cell–mediated suppression of CTL responses is prevented by the caspase inhibitor BD- FMK. Hilit Gur et al. Blood 2005;105:2585-2593 ©2005 by American Society of Hematology

6 CD34+ cell–mediated suppression of CTL responses is prevented by addition of anti–TNF-α but not by the addition of anti–TGF-β1. Hilit Gur et al. Blood 2005;105:2585-2593 ©2005 by American Society of Hematology

7 CD34+ cell–mediated suppression of CTL responses is prevented by addition of neutralizing anti–TNF-α mAb. Hilit Gur et al. Blood 2005;105:2585-2593 ©2005 by American Society of Hematology


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