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Dendritic cells accumulate in the bone marrow of myeloma patients where they protect tumor plasma cells from CD8 + T-cell killing by Patrizia Leone, Simona.

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Presentation on theme: "Dendritic cells accumulate in the bone marrow of myeloma patients where they protect tumor plasma cells from CD8 + T-cell killing by Patrizia Leone, Simona."— Presentation transcript:

1 Dendritic cells accumulate in the bone marrow of myeloma patients where they protect tumor plasma cells from CD8 + T-cell killing by Patrizia Leone, Simona Berardi, Maria Antonia Frassanito, Roberto Ria, Valli De Re, Sebastiano Cicco, Stefano Battaglia, Paolo Ditonno, Franco Dammacco, Angelo Vacca, and Vito Racanelli Blood Volume 126(12):1443-1451 September 17, 2015 ©2015 by American Society of Hematology

2 Strategy of flow cytometry analysis of immunostained whole blood and marrow samples (from MGUS and MM patients). Patrizia Leone et al. Blood 2015;126:1443-1451 ©2015 by American Society of Hematology

3 Frequency of DCs in whole blood and marrow samples (from 10 control subjects, 20 MGUS, and 20 MM patients). Patrizia Leone et al. Blood 2015;126:1443-1451 ©2015 by American Society of Hematology

4 Uptake of apoptotic tumor plasma cells by BM DCs and role of calreticulin in this process. Patrizia Leone et al. Blood 2015;126:1443-1451 ©2015 by American Society of Hematology

5 Costimulatory molecule expression and cytokine production by BM DCs (from 20 MGUS and 20 MM patients). Patrizia Leone et al. Blood 2015;126:1443-1451 ©2015 by American Society of Hematology

6 BM HLA-A2 tumor epitope–specific CD8+ T cells activated in vitro by BM DCs through crosspresentation. Patrizia Leone et al. Blood 2015;126:1443-1451 ©2015 by American Society of Hematology

7 CD28 expression and CD28-mediated interaction of tumor plasma cells with BM DCs (effect on proteasome subunit expression by tumor plasma cells). Patrizia Leone et al. Blood 2015;126:1443-1451 ©2015 by American Society of Hematology

8 Effect of BM DCs on tumor plasma cell susceptibility to CD8+ T cell–mediated killing in vitro. Patrizia Leone et al. Blood 2015;126:1443-1451 ©2015 by American Society of Hematology


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