1. Whatman New automated sample preparation system for HPLC L.P. Raman Technical Marketing Manager (Analytical Chemistry) Whatman, Inc, 9 Bridewell Place, Clifton, NJ 07013 Ingo Christ General Manager Chromsys LLC, PO Box 15131, Alexandria, VA 22309 Angelito Mendoza Chemistry Manager Gibraltar Labs, 122 Fairfield Road, Fairfield, NJ 07004

2. Whatman What is Mini-UniPrep™? Filtration & Storage device –Cuts sample preparation by 3x Unique, innovative design –500 µL Standardized –Compatible with autosamplers

3. Whatman How is Mini-UniPrep used

4. Whatman PrepBase automation platform Mini-UniPrep Rack (84 Slots) Diluter Compressor Stirrer Injection port

5. Whatman Automated sample prep used for... QC of Pharmaceutical tablets –Content uniformity (20) –Dissolution testing (12) –Blending (6) –QC of Raw materials (3) Assay of Cyclobenzaprine HCl Tablets

6. Whatman Automated Mini-UniPrep used for... Cosmetics QC of make-ups (lipstick, foundation) Sun-screen Pharmaceutical Topical Creams Arthritis Cream (Capsaicin) R&D Solubility studies Food and Beverage Juice Analysis of sugar content Label conformity Vitamin analysis

7. Whatman Results from our study Regular USP Vs Mini-UniPrep Automated Sample Prep –Assay of Cyclobenzaprine HCl Tablets »Performance results similar 116 % 25 % 12 % - Lab productivity - Labor costs - Consumables

8. Whatman Mini-UniPrep - HPLC Extractables

9. Whatman Cyclobenzaprine HCl - USP 27 NF:22 Finely powdered Tablets Transfer  10 mg Cyclobenzaprine to a flask Add 150 mL of 0.1 N HCl Stir for 15 minutes Dilute with 0.1 N HCl to 200 mL Filter Inject 10 µL into HPLC Take 5 mL in Syringe Place in standard 2 mL Vial In Autosampler Finely powdered Tablets Transfer  2.5 mg Cyclobenzaprine to MUP Add 0.5 mL of 0.1 N HCl Stir for 5 minutes Inject 10 µL into HPLC Compress MUP Place in standard in Autosampler

10. Whatman Mini-UniPrep automation platform (1) - Add HCl (3) Compress (2) Mix

11. Whatman Results (1) - Precision of method Objective - Mini-UniPrep Sample Prep provides precise results 5 vials of the standard prepared Mini-UniPrep Peak area of each injection was measured.

12. Whatman Results (2) - Linearity of method Acceptance: Correlation coefficient < 0.99; RSD - < 4% Objective - Establish linearity of Mini-UniPrep Sample 4 known dilutions of the standard prepared Mini-UniPrep Peak area of each injection was measured.

13. Whatman Results (3) - Sensitivity of method Objective: Mini-UniPrep Sample preparation provides good recovery at varying concentrations Tested at 3 spiked levels Acceptance criteria Recovery97-103% RSD < 4%

14. Whatman Results (4) - Automated Vs Regular

15. Whatman Results (4) - Automated Vs Regular Regular Method Automated Method

16. Whatman Automated Mini-UniPrep - Performance Data Summary Robust Scalable Sensitive Comparable to manual method

17. Whatman Benefits of automated Mini-UniPrep

18. Whatman Economic analysis …

19. Whatman Conclusions Mini-UniPrep™-PrepBase automated system Comparable to regular USP method Reduces sample preparation time Increases productivity To see Automated Min-UniPrep System in action come to Whatman Booth # 3479