1. Organic Analysis

2. Organization of Matter ATOMELEMENTCOMPOUND

3. Organization of Matter Atom –Smallest unit of matter –Composed of nucleus with protons and neutrons –Has electrons circling nucleus

4. Organization of Matter Element (ex. He) –About 110 different elements –Listed in Periodic Table –Substance composed of atoms –Elements vary in type of atom ie. Atomic mass ie. Atomic number

5. Organization of Matter Compound (ex. CO 2 ) –2 or more elements combined in fixed proportions

6. States of Matter Solid Liquid Gas SolidLiquid Gas Sublimation

7. Organic vs. Inorganic Qualitative vs. Quantitative

8. Chromatography Separates and tentatively IDs components of a mixture (ex. Street drugs that are diluted) Stationary Phase (solid or liquid) –Different substances have different affinities Mobile Phase which acts as solvent

10. Types of Chromatography Gas Chromatography (GC) High Performance Liquid Chromatography (HPLC) Thin Layer Chromatography (TLC)

11. Gas Chromatography Mobile Phase is a carrier gas Stationary Phase is a liquid coated onto a column Inject gaseous mixture Components having greater gas affinity will travel faster than those that like the liquid in the column

12. Gas Chromatography Pyrolysis= –Convert solids ex. Paint chips, fibers, plastics to a gas by heating to 500-1000 o C

13. Gas Chromatography Different components leave column at different times (retention time) A detector device converts substance to an electrical signal (Chromatogram). Signal peak proportional to amount of substance Sensitive to nanogram level

15. HPLC Liquid Moving Phase Solid stationary phase Done at room temp (Great for samples that are temp. sensitive)

16. SolventPump solvent at 1900 psi Inject Sample Column packed with beads Kids weave through crowds quickly large people slowed Electric Eye Shines light through sample Computer Records: Time Absorbance

17. HPLC

19. TLC Solid stationary phase –Silica plated glass plate Liquid mobile phase Apply liquid mixture to plate and put into solvent chamber. Solvent migrates up via capillary action

20. TLC Component with greatest solvent solubility migrates the fastest Run with known substances along side Develop to visualize separation Can’t definitively I.D. substances, not specific enough

21. TLC R f Value= Distance traveled by component Distance traveled by solvent Cheap, sensitive, fast

22. Electrophoresis Separation Technique No Mobile Phase Use electricity to move components Mixture loaded into an agar gel stationary phase Components separated based on charge and size

23. Electrophoresis

24. Enlargement of gel

25. Electrophoresis 10 min30 min60 min

26. Electrophoresis Can compare proteins and DNA fragments in crimes

27. Electrophoresis

28. Spectrophotometry =Technique to measure how much a substance absorbs light or other radiation

29. Spectrophotometry What is light?

30. Spectrophotometry Light is a form of energy in wave form. Also can be viewed as traveling as bundles of energy called photons

31. Spectrophotometry Who is this man?

32. Spectrophotometry

33. Part of the Electromagnetic Spectrum

34. Spectrophotometry Light Absorbance and Reflection” What color is being absorbed? What color is being reflected?

35. Spectrophotometer Concept

36. Types of Spectrophotometry Ultraviolet (So specific, it’s like a “fingerprint”) Infrared

37. Absorption Spectrum for Chlorophyll

38. Mass Spectrometry Ie. Blast electrons at unknown to make your unknown an ion Ions travel down the path based on their mass to charge ratio (m/z).

39. MS Results Methanol

40. Spectrophotometry Video http://www.youtube.co m/watch?v=xHQM4B bR040&feature=result s_video&playnext=1& list=PL967C03F9962 96203http://www.youtube.co m/watch?v=xHQM4B bR040&feature=result s_video&playnext=1& list=PL967C03F9962 96203

41. Mass Spectrometry http://www.youtube.co m/watch?v=J- wao0O0_qMhttp://www.youtube.co m/watch?v=J- wao0O0_qM

42. Thin Layer Chromatography http://www.youtube.co m/watch?v=yoYECM P_1iI&feature=relatedhttp://www.youtube.co m/watch?v=yoYECM P_1iI&feature=related

43. Gas Chromatography http://www.youtube.co m/watch?v=08YWhLT jlfo&feature=relmfuhttp://www.youtube.co m/watch?v=08YWhLT jlfo&feature=relmfu

44. Electrophoresis http://www.youtube.co m/watch?v=5eMsgidA Y5Ehttp://www.youtube.co m/watch?v=5eMsgidA Y5E