1. o-Raffinose cross-linked hemoglobin improves the hemostatic defect associated with anemia and thrombocytopenia in rabbits by David H. Lee, Leslie Bardossy, Nichole Peterson, and Morris A. Blajchman Blood Volume 96(10):3630-3636 November 15, 2000 ©2000 by American Society of Hematology

2. Cross-linked hemoglobin (CLHb) shortened the microvascular bleeding time in anemic rabbits.Microvascular bleeding times were measured in anemic rabbits 1 hour after infusion (left panel) of o-raffinose CLHb, 5% human serum albumin (HSA), phenylephrine (Phen... David H. Lee et al. Blood 2000;96:3630-3636 ©2000 by American Society of Hematology

3. CLHb shortened the microvascular bleeding time in thrombocytopenic rabbits.Microvascular bleeding times were measured in thrombocytopenic rabbits 1 hour after infusion (left panel) with o-raffinose CLHb, 5% HSA, Phen, or nothing (0). David H. Lee et al. Blood 2000;96:3630-3636 ©2000 by American Society of Hematology

4. CLHb decreased blood loss from thrombocytopenic rabbits.Blood loss from standardized ear incisions in thrombocytopenic rabbits was measured 1 hour (left panel) and 24 hours (right panel) after infusion of o-raffinose CLHb, 5% HSA, or Phen. David H. Lee et al. Blood 2000;96:3630-3636 ©2000 by American Society of Hematology

5. Extent of vasoconstriction induced by CLHb and Phen.Changes in microvascular ear perfusion in rabbits given an infusion ofo-raffinose CLHb (solid circles; n = 6), 5% HSA (open squares; n = 6), or Phen (open triangles; n = 5) were measured by using a laser D... David H. Lee et al. Blood 2000;96:3630-3636 ©2000 by American Society of Hematology

6. CLHb shortened the time to complete arterial occlusion in a rabbit model of carotid thrombosis.Carotid blood flow was measured in rabbits given an infusion ofo-raffinose CLHb (n = 10) or 5% HSA (n = 10). David H. Lee et al. Blood 2000;96:3630-3636 ©2000 by American Society of Hematology

7. CLHb infusion did not produce increased thrombin generation in vivo.Plasma thrombin- antithrombin concentrations were measured before (open bars) and 1 hour after (solid bars) a bolus infusion ofo-raffinose CLHb or 5% HSA or a continuous infusion of thrombop... David H. Lee et al. Blood 2000;96:3630-3636 ©2000 by American Society of Hematology

8. CLHb enhanced the aggregation of rabbit platelets stimulated with collagen.Impedance aggregometry was used to evaluate the effect ofo-raffinose CLHb on platelet aggregation in rabbit whole blood. David H. Lee et al. Blood 2000;96:3630-3636 ©2000 by American Society of Hematology

9. CLHb abolished the inhibitory effect of 3-morpholinosydnonimine (SIN-1) on platelet aggregation.The effect of o-raffinose CLHb on SIN-1–mediated platelet inhibition was evaluated by using impedance aggregometry on rabbit whole blood. David H. Lee et al. Blood 2000;96:3630-3636 ©2000 by American Society of Hematology

10. CLHb enhanced the aggregation of rabbit platelets stimulated with U46619 and arachidonic acid (AA) but not adenosine diphosphate (ADP). David H. Lee et al. Blood 2000;96:3630-3636 ©2000 by American Society of Hematology

11. The proaggregatory effect of CLHb was not attenuated by superoxide dismutase (SOD) and catalase.Impedance aggregometry was performed on rabbit whole blood stimulated with collagen (1 μg/mL) in the presence or absence of superoxide dismutase (200 U/mL), cata... David H. Lee et al. Blood 2000;96:3630-3636 ©2000 by American Society of Hematology