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APR and major R genes in wheat: current status at CSIRO Rohit Mago.

Published byCecil Gaines Modified over 8 years ago

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Presentation on theme: "APR and major R genes in wheat: current status at CSIRO Rohit Mago."— Presentation transcript:

1 APR and major R genes in wheat: current status at CSIRO Rohit Mago

2 Adult plant resistance genes for rust resistance Lr34, Yr18-7DS Lr46, Yr29-1BL Lr67, Yr46-4DL Lr68-7BL Yr49-3DS Sr2, Lr27, Pm-3BS SrGH-? Breeder markers Combinability Genetics and mechanism

3 Lr67, Yr46 Tc + Yr18 Tc + Yr46 Pakistan landrace BC into Tc = RL6077 Similar phenotype to Lr34/Yr18 (Ltn) more chlorosis/necrosis Single gene likely to be durable

4 cfd23 cfd71 wms4670 Lr67/Yr46 4 1 Lr67/ Yr46 on 4DL wms4866 4D Hiebert et al. TAG 2010 2 4DL 0.53-0.61 4DL 0.38-0.41 Herrera-Foessel et al. TAG 2010 Tc x RL6077 Av x RL6077

5 Yr49: APR for stripe rust on 3DS CM18xVig18 CM18xAvocet BC2 RILs gpw7080gwm161wms4741gwm1243NW2383 15 cM 7 cM22 cM 3 cM wms4741 gwm1243 3 cM~10 cM gpw7321 0.7 cM Yr49 centromere gwm161 QYr

6 Pathotype: 134 E16 A+17+27+ CM18Av-Yr49 Av-Yr18+Yr49 Av-Yr18 AvS-Yr18 NIL Avocet S Null Yr49 phenotype Yr49 more effective than Yr18, no Ltn single gene donor available Gene stacks developed

7 Simple diagnostic CAPS marker for Yr49 R S S S S S S S S S S S

8 Adult Plant resistance gene Sr-Glossy Huguenot (SrGH, Durum wheat) -SrGH+SrGH-Sr2 +Sr2 SrGH- a stem rust APR from tetraploid landrace is effective against Ug99 New mapping population with uniform maturity developed Not Sr2, can it be Combined with Sr2? (Hare RA 1997)

9 Stem rust resistance Sr2 is linked to leaf rust resistance gene Lr27 and powdery mildew resistance Mago et al TAG (2011) 123(4) : 615-623 CS-Hope M72-2 Hartog 37 ISRTN 07

10 Marquis GCACAAGCTCTAATTTCTTTGGAATCGTGAGTGTTGGATGTCTCGCACACCTAGCTTAAT Fultz GCACAAGCTCTAATTTCTTTGGAATCGTGAGTGTTGGATGTCTCGCACACCTAGCTTAAT Hope GCACAAGCTCTAATTTCTTTGGAATCATGAGTGTTGGATGTCTCGCACACCTAGCTTAAT Emmer GCACAAGCTCTAATTTCTTTGGAATCATGAGTGTTGGATGTCTCGCACACCTAGCTTAAT CsSr2: A new diagnostic marker for Sr2 BspHI Mago et al TAG (2011) 122:735–744 R S S R 172bp 112bp 53bp 225bp Hope Sr2, Lr27, Pm, PBC

11 CA640157RGA_1gwm533 BEX_2 (570 kb) RKO_1 Sr2, Lr27, Pm, PBC Centromere DOX_1 DOX_1 (590 kb) RGA_1 (704 kb) Unk1 (466 kb) Unk2 (393 kb) RKO_2 (297 kb) MSF_2(130 kb) ROK_1 (21 kb) 0.0015 cM ~0.3 cM ~ 580 kb CS physical (part of contig11) Current status of physical mapping at Sr2 locus C12 D7 C9 D10 A8 p4D6 B2 p3A5 p2B5 DOX_1 CS-Hope 3B -MTP p1E9 99C5 121B5 6K2 71B4-T7 226H3 Gap? 315B7 Large insertion in ‘Hope’ RKO_1 RGA_1

12 Race specific stem rust resistance genes Sr22 - T. monococcum Sr32 - T. speltoides Sr33 - A. tauscii Sr39 - T. speltoides Sr45 - A. speltoides Sr50 - S. cereale Breeders markers Gene stacking Genetics and cloning

13 Probe ABG58 ABG2 ABG358 ABG5 ABC454 BCD111 ABG72 ABC252 70 v 2.0 70 v1.0 122c Sr32 [C77.19, C82.1 (2B)] Sr2S#1 [C82.2, C82.3, C82.4 (2D)] Wheat –T. speltoides recombinants* *C82.2 recombinants produced by Dr. Ian Dundas R R R S S S R R S R Marker for Sr2S#1 Infection Type 2= ;1

14 Marker development for Sr32 S R S S S  Diagnostic Multiplex PCR marker for Sr2S#1  Development of marker for Sr32 in process

15 Plant Industry Sutha Chandramohan Jeff Ellis Raja Kota* Evans Lagudah Rohit Mago Sambasivam Periyannan Wendelin Schnippenkoetter Wolfgang Spielmeyer Linda Tabe Libby Viccars Xiaodi Xia Univ of Adelaide Ian Dundas PBI, Cobbitty Urmil Bansal Harbans Bariana Bob McIntosh Robert Park Colin Wellings Funding GRDC International Collaborators CIMMYT, Mexico CNRGV-INRA, France Univ of Olomouc, Czech Republic Univ North Carolina Murdoch Univ. Rudi Appels James Breen* Acknowledgments

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