1. Lactadherin inhibits enzyme complexes of blood coagulation by competing for phospholipid-binding sites by Jialan Shi, and Gary E. Gilbert Blood Volume 101(7):2628-2636 April 1, 2003 ©2003 by American Society of Hematology

2. Competition for factor VIII– and factor V–binding sites by lactadherin or annexin V.(A) First, 4 nM fluorescein-labeled factor VIII was mixed with the indicated concentration of competitor, lactadherin ( ▴ ) or annexin V (●), in the presence of 1.5 mM Ca++. Jialan Shi, and Gary E. Gilbert Blood 2003;101:2628-2636 ©2003 by American Society of Hematology

3. Relationship of vesicle curvature and PS content to inhibition of the prothrombinase complex by lactadherin or annexin V.Sonicated vesicles ( ▴ and ▵ ), extruded vesicles (● and ○), and LMVs (▪ and ■) contained 4% PS (top row) or 15% PS (bottom row). Jialan Shi, and Gary E. Gilbert Blood 2003;101:2628-2636 ©2003 by American Society of Hematology

4. Relationship of vesicle curvature and PS content to inhibition of the factor Xase complex by lactadherin or annexin V.Sonicated vesicles ( ▴ and ▵ ), extruded vesicles (● and ○), and LMVs (▪ and ■) contained 4% PS (upper row) or 15% PS (lower row). Jialan Shi, and Gary E. Gilbert Blood 2003;101:2628-2636 ©2003 by American Society of Hematology

5. Inhibition of the factor VIIa–tissue factor complex by lactadherin or annexin V.Recombinant tissue factor was reconstituted into vesicles of PS-PE-PC composition of 4:20:76 by detergent dialysis, with a tissue factor-PL ratio of 1:7500. Jialan Shi, and Gary E. Gilbert Blood 2003;101:2628-2636 ©2003 by American Society of Hematology

6. Competition for factor IXa–binding sites by lactadherin.The active site histidine of factor IXa was derivatized with fluorescein-Glu-Gly-Arg chloromethyl ketone as described in “Materials and methods.” First, 4 nM fluorescein-labeled factor IXa was mixed wi... Jialan Shi, and Gary E. Gilbert Blood 2003;101:2628-2636 ©2003 by American Society of Hematology

7. Relationship of inhibition by lactadherin to phospholipid concentration.The function of factor Xase complex (A) and prothrombinase complex (B) on sonicated vesicles was evaluated at 0.1 (●), 0.5 (■), and 2.5 ( ▴ ) μM phospholipid concentrations in the presenc... Jialan Shi, and Gary E. Gilbert Blood 2003;101:2628-2636 ©2003 by American Society of Hematology

8. Inhibition of whole blood prothrombin time by lactadherin or annexin V.Fresh whole blood was anticoagulated with 10 mM citrate and 25 μg/mL corn trypsin inhibitor. Jialan Shi, and Gary E. Gilbert Blood 2003;101:2628-2636 ©2003 by American Society of Hematology