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IGEM BOOT CAMP SUMMER CAMP 2014. LAB-DAY1  Introduced to basic lab equipments.  An autoclave is a device used to sterilize equipment and supplies by.

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Presentation on theme: "IGEM BOOT CAMP SUMMER CAMP 2014. LAB-DAY1  Introduced to basic lab equipments.  An autoclave is a device used to sterilize equipment and supplies by."— Presentation transcript:

1 IGEM BOOT CAMP SUMMER CAMP 2014

2 LAB-DAY1  Introduced to basic lab equipments.  An autoclave is a device used to sterilize equipment and supplies by subjecting them to high pressure saturated steam at 121 °C for around 15–20 minutes...

3 LAB-DAY2  Prepration of the CaCl2 + MgCl2 solution used to create competent cell so that plasmid can be introduced into the cell.

4 LAB-DAY3  Autoclave of equipments.  Prepared 70% v/v alcohol solution,that will be used sterlizing in future before starting the other process in the experiment.  Ecoli bacteria will be cultured under incubator in a test tube containing LB(lysogeny broth).

5 PROGRESS IN OTHER AREAS  We have been divided into two teams and are studying previous igem project to get the sense of how and what they will do to participate in igem2015.  PROJECT INCLUDE: use of biofilm for water treatment and ways to sort bacteria with choices of light sources

6 PROGRESS IN OTHER AREAS  An overview of techniques such as Gibson Assembly,PCR.  Introduced to Holliday Junction.

7 FUTURE AIM Prepration of competent cell Standard heat-shock transformation of chemically competent bacteria Culture transformed cell in proper conditions under incubator

8 FUTURE AIM Study more about igem project Start thinking about how to begin the project for igem THANK YOU


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