1. Chapter 5: Equations Equations are a way to describe relationships using mathematical symbols. V = IR voltagecurrent resistance

2. Chapter 5: Equations Equations are a way to describe relationships using mathematical symbols. °F = 1.8(temperature in °C) + 32 constants variables

3. Chapter 5: Equations An application: centrifugation

4. Chapter 5: Equations An application: centrifugation

5. Chapter 5: Equations An application: centrifugation

6. Chapter 5: Equations An application: centrifugation RCF = 11.2 X r RPM 1000 ( ) 2

7. Chapter 5: Equations An application: centrifugation How many g-forces on the carnival ride?

8. Chapter 5: Equations An application: centrifugation radius = 13 ft. = ? cm 13 ft. (12 inches) (2.54 cm) (1 ft.) (1 inch) = 396.24 cm rpm = 16 X X

9. Chapter 5: Equations An application: centrifugation RCF = 11.2 X r RPM 1000 ( ) 2 RCF = 11.2 X (396 cm) 16 1000 ( ) 2 RCF = 1.13 x g

10. Chapter 5: Equations lab activity: centrifugation 1. measure the radius of the rotor in centimeters

11. Chapter 5: Equations lab activity: centrifugation 2. balance the rotor with two microfuge tubes 3. tighten the lid and close cover

12. Chapter 5: Equations lab activity: centrifugation 4. set the speed to 5,000 rpm and time to 5 minutes and push “start” 5. calculate rcf using your values of radius and rpm RCF = 11.2 X r RPM 1000 ( ) 2

13. Chapter 5: Equations lab activity: centrifugation 6. push the rpm/rcf button…was your calculation correct? 7. use rpm and rcf values from the machine to calculate radius of rotor RCF = 11.2 X r RPM 1000 ( ) 2