1. Protein Synthesis

2. One Gene – One Enzyme

3. Protein Synthesis

8. Transcription DNA  mRNA Occurs in the nucleus Translation mRNA  tRNA  AA Occurs at the ribosome

9. Triplets (Codons)

10. Genetic Code

11. Transcription

15. Initiation Promoter TATA Box Initiation Site Transcription factor RNA Polymerase AUG

16. Elongation RNA Polymerase Adds nucleotides to the 3’ end

17. Termination Proceeds until RNA polymerase transcribes a terminator sequence UAA UAG UGA

18. RNA Processing 5’ guanine cap 3” Poly (A) tail

19. RNA Splicing Introns Exons

20. Spliceosomes snRNPs Small nuclear ribonucleoproteins

21. Introns???? Regulation One gene can code for alternate polypeptides Evolution Domains – structural and functional regions

22. Translation

23. tRNA

24. Aminoacyl- tRNA Synthetase

25. Ribosomes Small subunit Large subunit P site (peptidyl-tRNA) A site (Aminoacyl-tRNA) E site (Exit site)

26. Initiation

27. Elongation

28. Termination

29. Polyribosomes

30. Point Mutation

31. Base Pair Insertion or Deletion Frameshift Missense – new AA Nonsense – stop

32. Point Mutation Base Pair Substitution