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Réalisation de clones mitotiques

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Presentation on theme: "Réalisation de clones mitotiques"— Presentation transcript:

1 Réalisation de clones mitotiques
Flp = Flippase FRT = Flippase Recombination Target

2 Comment visualiser facilement les deux clones homozygotes sauvages et mutants ?

3 Reporter 1 = UAS-CD8::GFP, Suppressor 1 = UAS-RNAi-GFP
Reporter 2 = UAS-Cd2::RFP, Supressor 2 = UAS-RNAi-Cd2 + tub-gal4 Twin-spot MARCM to reveal the developmental origin and identity of neuronsHung-Hsiang Yu, Chun-Hong Chen, Lei Shi, Yaling Huang & Tzumin LeeNature Neuroscience 12, (2009)

4 Tzumin Lee, Nature Methods 6, 566 - 568 (2009)

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