1. Impacts of Soil-Transmitted Helminths Katharina Stracke, PhD student, Year 1, The Walter and Eliza Hall Institute, MDHS @kastracke @WEHI_research @UniMelbMDHS Overview Higher infection prevalence than malaria! Symptoms a) Acute = diarrhea b) Chronic = malnutrition stunting wasting iron deficiency anaemia Overview Higher infection prevalence than malaria! Symptoms a) Acute = diarrhea b) Chronic = malnutrition stunting wasting iron deficiency anaemia Methods Stool + specimen + data sampling DNA isolation Multiplexed-tandem PCR Sequencing Regression analysis Methods Stool + specimen + data sampling DNA isolation Multiplexed-tandem PCR Sequencing Regression analysis Hypotheses  High worm burden in children <5 years  High rates of stunting, wasting and malnutrition  Multi-pathogen infections are common  Emerging drug resistances (Benzimidazoles) Hypotheses  High worm burden in children <5 years  High rates of stunting, wasting and malnutrition  Multi-pathogen infections are common  Emerging drug resistances (Benzimidazoles) Images: A. lumbricoides (A), Hookworm (B) and T. trichiura (C) A: B: Alan J Cann, accessed on 18 Aug 2016 C: Johann Gottfried Bremser, accessed on 26 Jul 2016 Images: A. lumbricoides (A), Hookworm (B) and T. trichiura (C) A: B: Alan J Cann, accessed on 18 Aug 2016 C: Johann Gottfried Bremser, accessed on 26 Jul 2016 Global infection prevalence of STH in percent (Pullan et al. 2014). Aim Characterization of STH infection, malnutrition and co-morbidity on child growth among Karen refugee pre-school children from Tha Song Yang district, Tak province, Thailand. Aim Characterization of STH infection, malnutrition and co-morbidity on child growth among Karen refugee pre-school children from Tha Song Yang district, Tak province, Thailand. AB C 0.2 billion infections vs 2 billion infections D E FG

2. References Pullan, R. L., Smith, J. L., Jasrasaria, R., & Brooker, S. J. (2014). Global numbers of infection and disease burden of soil transmitted helminth infections in 2010. Parasites & vectors, 7(1), 1-19. doi:10.1186/1756-3305-7-37 Image A: Lwp Kommunikáció, Ascaris lumbricoides, 27th February 2012, Downloaded from: https://www.flickr.com/photos/lwpkommunikacio/14991711507/in/photolist-pVvqaz-rDo9Ck-pVvoFn-31PQXz-q8gy1b-fkfeJ2-pDj3gZ-pTzwnU- nxM4gF-g3TndH-cHgbcJ-rGd1ef-PAc1x-cHgbpj-f6qmdq-cHg8BS-cHg4hQ-cHgaWq-cHg28d-cHfURE-oQLrwB-f4BLku on 18th August 2016, Licensed under: https://creativecommons.org/licenses/by/2.0/ Image B: Alan J Cann, Hookworm, 30th May 2008, Downloaded from: https://www.flickr.com/photos/ajc1/2535649627/ on 18th August 2016, Licensed under: https://creativecommons.org/licenses/by-nc/2.0/ Image C: Johann Gottfried Bremser, Trichuris trichiura, 1st Jan 1831, Downloaded from: https://commons.wikimedia.org/wiki/File:Trichuris_trichiura.jpg on 26th July 2016, Licensed under: https://commons.wikimedia.org/wiki/Commons:Licensing Image D: Pullan, R. L., Smith, J. L., Jasrasaria, R., & Brooker, S. J. (2014). Global numbers of infection and disease burden of soil transmitted helminth infections in 2010. Parasites & vectors, 7(1), 1-19. doi:10.1186/1756-3305-7-37 Image E: Unicode, Emoji, 28th October 2015, Downloaded from: https://commons.wikimedia.org/wiki/Emoji on 26th July 2016, Licensed under: https://commons.wikimedia.org/wiki/Commons:Licensing and http://www.unicode.org/emoji/images.html Image F: Elias Schaefer, Mosquito, July 2016, Downloaded from: https://pixabay.com/p-1465064/?no_redirect on 18th August 2016, Licened under: https://creativecommons.org/publicdomain/zero/1.0/deed.en Image G: Katharina Stracke, Worm, 26th July 2016 Acknowledgements Aaron JexHarin KarunajeewaSuparat Phaunonakoon Melanie BahloRebecca TraubAll participants and their families Samantha Emery Brendan AnsellMahidol Regional Hospital & Mahidol University Louise Baker Nijoy John Camille Awburn Population Health and Immunity Division References Pullan, R. L., Smith, J. L., Jasrasaria, R., & Brooker, S. J. (2014). Global numbers of infection and disease burden of soil transmitted helminth infections in 2010. Parasites & vectors, 7(1), 1-19. doi:10.1186/1756-3305-7-37 Image A: Lwp Kommunikáció, Ascaris lumbricoides, 27th February 2012, Downloaded from: https://www.flickr.com/photos/lwpkommunikacio/14991711507/in/photolist-pVvqaz-rDo9Ck-pVvoFn-31PQXz-q8gy1b-fkfeJ2-pDj3gZ-pTzwnU- nxM4gF-g3TndH-cHgbcJ-rGd1ef-PAc1x-cHgbpj-f6qmdq-cHg8BS-cHg4hQ-cHgaWq-cHg28d-cHfURE-oQLrwB-f4BLku on 18th August 2016, Licensed under: https://creativecommons.org/licenses/by/2.0/ Image B: Alan J Cann, Hookworm, 30th May 2008, Downloaded from: https://www.flickr.com/photos/ajc1/2535649627/ on 18th August 2016, Licensed under: https://creativecommons.org/licenses/by-nc/2.0/ Image C: Johann Gottfried Bremser, Trichuris trichiura, 1st Jan 1831, Downloaded from: https://commons.wikimedia.org/wiki/File:Trichuris_trichiura.jpg on 26th July 2016, Licensed under: https://commons.wikimedia.org/wiki/Commons:Licensing Image D: Pullan, R. L., Smith, J. L., Jasrasaria, R., & Brooker, S. J. (2014). Global numbers of infection and disease burden of soil transmitted helminth infections in 2010. Parasites & vectors, 7(1), 1-19. doi:10.1186/1756-3305-7-37 Image E: Unicode, Emoji, 28th October 2015, Downloaded from: https://commons.wikimedia.org/wiki/Emoji on 26th July 2016, Licensed under: https://commons.wikimedia.org/wiki/Commons:Licensing and http://www.unicode.org/emoji/images.html Image F: Elias Schaefer, Mosquito, July 2016, Downloaded from: https://pixabay.com/p-1465064/?no_redirect on 18th August 2016, Licened under: https://creativecommons.org/publicdomain/zero/1.0/deed.en Image G: Katharina Stracke, Worm, 26th July 2016 Acknowledgements Aaron JexHarin KarunajeewaSuparat Phaunonakoon Melanie BahloRebecca TraubAll participants and their families Samantha Emery Brendan AnsellMahidol Regional Hospital & Mahidol University Louise Baker Nijoy John Camille Awburn Population Health and Immunity Division