1. Characterization of Antifungal activity of some Moroccan medicinal plants extracts against candida spp
A.ABUDUNIA*1, 2, 4, M.ANSAR2,,J.TAOUFIK2, Y.RAMLI2, Mr.ESSASSI3,F.Ohmani4, A.IBRAHIMI1 and K .KHEDID4
1Biotechnology Lab (Med-Biotech), FMPR, UMVR, 2Department of Medicinal Chemistry laboratory, FMPR, UMVR, 3Department of Heterocyclic Organic Chemistry Laboratory, FSR, UMVR, 4Department of microbiology, INH, Rabat
INTRODUCTION
Candida is a common microflora yeast in humans and an opportunistic fungal infection which is often found in compromised immune systems and causing candidiasis in the case of an overgrowth.
In traditional medicine, medicinal herbs and plant products are used in treating a wide spectrum of infections and other diseases. The aim of this study was to assess the antifungal activity of three extracts from Moroccan Medicinal Plants (Syzygium aromaticum, Eucalyptus rossi, Rosaminus officinalis, Thymus vulgaris and Calendula arvensis) against 6 species of Candida spp.
Flowers
Calendula Arvensis
Candida Glabatra 1
Zone of Inhibition
Methanolic
(Soxhlet)
Well Diffusion Method
Candida Glabatra 2
MATERIAL
AND
METHODS
Aerial part
Syzygium Aromaticum
Candida Famata 1
Hexanolic
(Soxhlet)
MIC
Used parts
Leaves
Plants
Eucalyptus Rossi
Candida Famata 2
Aquoeus
(Maceration)
Leaves and Flowers
Candida Tropicalis 1
Rosmarinus Officinalis
MBC
Candida Tropicalis 2
Leaves and Flowers
Thymus Vulgaris
Extracts
Methods
Parameters
Strains
GF Antifungal activity of extracts in term of inhibition zone (IZ) in mm
ECA
ESA
EER
ERO
ETV ME HE AE
CT1 14 23 25 24 20 16 28 10 9 18 17 19
CT2 22 13 15
CF1 12 29 11 8
CF2 30
CG1
CG2 26 21
The total Of inhibition 34
142
136
114
123 87
160 80 71
117 86 76
MDI%
23.7
22.7
20.5
14.5
26.7
13.3
11.8
19.5
14.3
12.7
Table1: Antifungal activity of different extracts of plants medicinal
GFS: Group fungal strain; CT1: Candida Tropicalis, CT2: Candida Tropicalis, CF1: Candida Famata, CF2: Candida Famata, CG1: Candida Glabrata, CG2: Candida Glabrata, ECA:Extract of Calendula arvensis, ESA: Extract of Syzygium Aromaticum, EER: Extract of Eucalyptus Rossi, ERO: Extract of Rosmarinus Officinalis, ETV: Extract of Thymus Vulgaris. MDI: The mean diameter of inhibition
The fungal
Strain tested
ESA
EER
ERO
ETV
MIC μg/ml
MFC μg/ml
C.T1 2 6 3
1.5 4
0.7
C.T2
2.5
0.5 10 5
C.F1
4.5
C.F2
C.G1
C.G2
3.75
Table 2: Determination of Minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of methanolic extracts of different plants.
RESULTS
The results showed that the maximum antimycotic activity was demonstrated by aqueous extracts of Eucalyptus Rossi, MIC values of methanolic extracts were between µg/ml, the obtained results show an antifungal potency of these extracts and were bactericidal for all fungal tested.
While the extracts of Calendula arvensis exhibited no activity on these species except methanolic extract showed activity on Candida tropicalis and Candida famata1.
CONCLUSION
Our results confirm that these plants extract which can be used to cure mycotic infections can have also pharmaceutical and preservatives properties.