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Biochemistry Review Biology is the study of living things. Organic or Biochemistry is the study of the chemical reactions in living things.
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What is the smallest unit of matter? An Atom
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Atoms- What area is positive and what area is negative? The nucleus is positive The surrounding electron orbitals are negative Where is the mass? In the nucleus
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Atoms Atomic Number= # of p + Atomic Mass= p + + n 0 Charge= p + - e - First Energy Level= can hold.. 2 electrons Second Energy level can hold.. 8 Electrons
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What is a molecule? Atoms bonded together with covalent bonds
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How are ions and isotopes same/different? They are both atoms and the number of protons stays the same, BUT Ions have different charges (different number of electrons) Isotopes have different masses (different number of neutrons)
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What makes a molecule polar? Uneven sharing of electrons gives the molecule partially negative areas and partially positive areas Give an example of a polar molecule. Water – H 2 0= Oxygen has a partial negative charge, hydrogen has a partial positive charge.
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What is a compound? A substance made of two or more different elements. Could be bonded by ionic or covalent bonds
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What is the most common element in living things? Carbon It is present in all four of the biomolecules Which biomolecule contains nitrogen? Protein
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Let’s talk about bonds! What are the three types we have covered? Ionic, Covalent, and Hydrogen bonds
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A Bond formed when molecules share electrons Covalent Bonds
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A Bond between two oppositely charged atoms or molecules (one positive one negative) Ionic bonds
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An intermolecular force between polar molecules A Hydrogen Bond
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What is the relative strength of these bonds? Covalent/ Ionic >>>>>>>Hydrogen Bonds
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pH scale 1-14 What does it measure? The acidity or basicity of a solution 7 is neutral Acids are … Below 7 Bases are … Above 7
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Four Categories of Biomolecules Proteins Carbohydrates Lipids Nucleic Acids These are very large molecules so we call them Macromolecules!
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Carbohydrates- What do we know? Ratio of C:H:O is 1:2:1 Monomers are: saccharides-simple sugars Polymers are: polysaccharides- starches
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Lipids Monomers are glycerol and fatty acids Polymers are Trigycerides- a glycerol with three fatty acid chains Lipids can be fats or oils, They are nonpolar- don’t mix with water Saturated fats are solid (butter, lard) Unsaturated are oils (olive, vegetable)
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Proteins Monomer is an amino acid (20 types), each amino acid has an R group on its central carbon. Polymers are polypeptides, two or more amino acids bonded together
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What is the process that builds polymers from monomers? Dehydration Synthesis (synthesize is to make) Remember this process gives off a molecule of …… H 2 0
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Breaking down polymers into monomers is called Hydrolysis- “Lysis” is to break What molecule is needed for this to happen? H 2 0 must be present and breaks into its parts to replace the H’s and O
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In our lab, we tested for different biomolecules: Iodine- What does it test for and what’s a positive reaction? Iodine tests for starch- If starch is present it turns.. Blue-Black
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What tests for simple sugars (Glucose)? Benedicts plus heat (it starts out blue) Positive reaction is a change to green, yellow or red depending on how much glucose is present
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What does Biuret test for? Proteins What color change do you see if protein is present? It changes from blue to a purple-lavender to violet.
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How did we check for Lipids? Paper towel test Translucent (can see through it) when lipids were present.
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This is the end. Best of Luck!
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