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Dim ‐ 2 encodes a DNA methyltransferase responsible for all known cytosine methylation in Neurospora by Elena Kouzminova, and Eric U. Selker EMBO J. Volume.

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Presentation on theme: "Dim ‐ 2 encodes a DNA methyltransferase responsible for all known cytosine methylation in Neurospora by Elena Kouzminova, and Eric U. Selker EMBO J. Volume."— Presentation transcript:

1 dim ‐ 2 encodes a DNA methyltransferase responsible for all known cytosine methylation in Neurospora by Elena Kouzminova, and Eric U. Selker EMBO J. Volume 20(15):4309-4323 August 1, 2001 ©2001 by European Molecular Biology Organization

2 The dim ‐ 21 mutation is complemented in heterokaryons. Elena Kouzminova, and Eric U. Selker EMBO J. 2001;20:4309-4323 ©2001 by European Molecular Biology Organization

3 Chromosome walk to the dim ‐ 2 gene. Elena Kouzminova, and Eric U. Selker EMBO J. 2001;20:4309-4323 ©2001 by European Molecular Biology Organization

4 Complementation of dim ‐ 2 mutation. Elena Kouzminova, and Eric U. Selker EMBO J. 2001;20:4309-4323 ©2001 by European Molecular Biology Organization

5 The predicted amino acid sequence of the Dim ‐ 2 polypeptide. Elena Kouzminova, and Eric U. Selker EMBO J. 2001;20:4309-4323 ©2001 by European Molecular Biology Organization

6 Sequence alignment of the C ‐ terminal domains of Mus musculus Dnmt1 (DDBJ/EMBL/GenBank accession No. P13864), Ascobulus immersus Masc2 (accession No. CAB09661), Arabidopsis thaliana MET1 (accession No. P34881) and Neurospora crassa Dim ‐ 2 (accession No. AF348... Elena Kouzminova, and Eric U. Selker EMBO J. 2001;20:4309-4323 ©2001 by European Molecular Biology Organization

7 Analysis of dim ‐ 2 transcription by RT–PCR. Elena Kouzminova, and Eric U. Selker EMBO J. 2001;20:4309-4323 ©2001 by European Molecular Biology Organization

8 Construction and characterization of the Δdim ‐ 22 allele. Elena Kouzminova, and Eric U. Selker EMBO J. 2001;20:4309-4323 ©2001 by European Molecular Biology Organization

9 DNA methylation in Δdim ‐ 2 strains expressing various dim ‐ 2 constructs. Elena Kouzminova, and Eric U. Selker EMBO J. 2001;20:4309-4323 ©2001 by European Molecular Biology Organization


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