1. Real time metagenomics Ross Overbeek Bob Olson Terry Disz Liz Dinsdale

2. How much so far Total: 3,348 metagenomes 318,630,847 sequences 82,945,869,083 bp (83 Gbp) Largest metagenome: 729 Mbp, 11,719,618 reads Public: 393 Metagenomes 54,306,078 sequences 22,160,008,455 bp (22 Gbp) Compute time (on a single CPU): 1,575,971 hours = 65,665 days = 179 years

3. We can do it! Click Here!

4. GS20 microbial metagenomes from coral water around Kingman OLDNEW

5. Titanium microbial metagenome coral water from Kiritimati OLDNEW

6. Vector dot products 8,920 dimensional space (one for each function) Compare real time to BLAST based Compare within a sample (intrinsic) Compare sample to genomes (extrinsic)

7. RT vs BLASTX

8. Intrinsic (how well does a subsample represent the whole sample)

9. Extrinsic (how well do complete genomes represent the metagenomes)

10. What's next for real time metagenomics?

12. BACK!