1. Correlation between the increased number of PML-NBs and the increased HBV DNA copy number in response to chemotherapy and irradiation-induced DNA damage.
Correlation between the increased number of PML-NBs and the increased HBV DNA copy number in response to chemotherapy and irradiation-induced DNA damage. Human hepatoma 1.3ES2 cells were treated with etoposide (20 μmol/L) or doxorubicin (1.5 μmol/L) for 30 min or irradiation (5 Gy) in one fraction to induce DNA damage. A. Increases in the number of PML-NBs in individual cells over time after DNA damage. To reflect the dynamic change of the number of PML-NBs with cell cycle, PML-NBs were counted in at least 50 cells in the same area per time point regardless of cell cycle distribution. B. Increases in the number of HBV DNA molecules secreted into the medium over time after DNA damage. C. Cell growth curves following DNA damage. Cell number, viability, and lysis were determined by the trypan blue exclusion method.
Yih-Lin Chung, and Tzung-Yuan Tsai Mol Cancer Res 2009;7:
©2009 by American Association for Cancer Research