1. Sealing Ability and Bioreactivity of an
Experimental Dentin Desensitizing Agent
Cheuk, J.1, Anders, R.1, Theriot, S.2, Falster, A.3, Sarkar, N. K.1
1Louisiana State University School of Dentistry, New Orleans, LA, 70119
2Veteran Affairs Medical Center, New Orleans, LA, 70112
3University New Orleans, New Orleans, LA, 70148
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Energy dispersive x-ray analysis
INTRODUCTION
RESULTS
CONCLUSIONS
Dentin sensitivity is an uncomfortable dental condition experienced by many adults. It results from the exposure of dentinal tubules to external physicochemical stimuli. Occluding exposed tubules with therapeutic agents is one means of treating dentine sensitivity. Of various commercial products available for this purpose, only a few have been found to be effective.
SEM and X-Ray Analysis of Dentin Surfaces as a Function of Treatments
The experimental calcium silicate (CS) based desensitizing agent when applied to etched dentin surfaces rapidly forms an adherent coating and seals open dentinal tubules.
The siliceous coating is metastable in a physiological aqueous environment.
When exposed to PBS, it undergoes apatitic transformation in situ – an indication of its biomineralizing ability.
The biomineralizing property of the coating is of significance not only to desensitization but also in control and prevention of caries.
Tubules (T)*
EDTA reveals the dentinal tubules (T). Intertubular matrix (M) is less affected by EDTA. Original grinding marks are visible. X-ray analysis shows the area to contain mainly Ca, P, and O - essential ingredients in tooth minerals. T
EDTA TREATED
OBJECTIVES M
The aims were to study: (1) the efficacy of an experimental desensitizing agent in occluding dentinal tubules and (2) the reactivity of sealed surfaces in a physiological saline solution.
METHODS
Tubules (T)*
Coating occludes the tubules and covers the intertubular matrix. Original grinding scratches are masked by a Ca-Na-Si-PO4 coating.
The experimental material was a calcium silicate (CS) based gel.
Flat buccolingual dentinal sections (n = 6) obtained from extracted human molars were final ground with 1200 grit SiC paper, etched with EDTA, and cleaned ultrasonically in distilled water. Etching was done to exposed tubules.
The CS gel was painted onto the etched dentine surface.
The treated specimens were stored in a phosphate buffer saline solution (PBS) at 37°C for 1 m. This procedure is performed to mimic intraoral exposure.
Following storage, specimens were cleaned ultrasonically in distilled water, and dried at 37°C.
Specimens (n= 2 each) representing ground, etched, treated, and PBS exposed conditions were examined by SEM and EDXA.
GEL COATED
REFERENCES T
Pashley, D.H. Dentin permeability, dentin sensitivity, and treatment through tubule occlusion. J Endodont 1986; 12:465.
Markowitz, K. and Pashley, D.H. Discovering new treatments for sensitive teeth: the long path from biology to therapy. J Oral Rehab 2008; 35:
Ling, T.Y. and Gillam, D.G. The effectiveness of desensitizing agents for the treatment of cervical dentine sensitivity (CDS) – a review. Perio Abstracts 1996; 44 (1): 5-12.
Addy, M. et al. Tooth Wear and Sensitivity. Informa Healthcare 1st ed M
Tubules (T)*
Coating in the matrix region dissolves exposing the original scratches. Within the tubules, the coating is transformed in situ. The transformation product is virtually Si-free and contains mainly Ca, P, and O. M
PBS TREATED T
*For all treatments, the x-ray spectra from the matrix area, M, that is were essentially similar to that from the tubular region, T.