1. MAMMARY GLAND This resource is licensed under the Creative Commons Attribution Non-Commercial & No Derivative Works LicenseCreative Commons Attribution Non-Commercial & No Derivative Works License

2. Objectives For the Gross anatomy and Histology of the mammary gland. Students should be able to: 1.Appreciate the differences in duct numbers per teat. 2.Identify the mammary glands and their main features in all domestic species. 3.Identify their blood vessels, nerves and lymph ducts; appreciate internal structure features. 4.Identify the features of the microscopic structure of active and inactive mammary glands.

3. SLIDE 98 Mammary gland - inactive At low magnification identify : 1. area of tubules. 2. interlobular connective tissue. 3. lactiferous ducts. 4. teat. 5. surface of skin. 2.5 mm

4. SLIDE 98 Mammary gland - inactive At low magnification identify : 1. area of tubules. 2. interlobular connective tissue. 3. lactiferous ducts. 4. teat. 5. surface of skin. 2.5 mm 1 2 3 4 5 1 2 Connective tissue is stains blue/green in this triple stained section.

5. SLIDE 98 Mammary gland - inactive Area of teat identify : lactiferous ducts. lactiferous sinuses. teat sinus. 1.0 mm

6. SLIDE 98 Mammary gland - inactive Area of teat identify : lactiferous ducts. lactiferous sinuses. teat sinus. 1.0 mm teat sinus lactiferous ducts lactiferous sinus ‘star’ shaped appearance connective tissue

7. SLIDE 98 Mammary gland - inactive Opening of teat sinus, identify : teat sinus. glands opening into sinus. keratinised, stratified squamous epithelium. 250 µm

8. SLIDE 98 Mammary gland - inactive Opening of teat sinus, identify : teat sinus. glands opening into sinus. keratinised, stratified squamous epithelium. 250 µm teat sinus glands keratinised stratified squamous epithelium fold in section teat canal fold

9. SLIDE 98 Mammary gland - inactive Identify : lobules interlobular duct system interlobular connective tissue 0.5 mm

10. SLIDE 98 Mammary gland - inactive Identify : lobules interlobular duct system interlobular connective tissue 0.5 mm L : lobules interlobular ducts opening to lactiferous ducts Ct : interlobular connective tissue lactiferous duct L L L Ct

11. SLIDE 98 Mammary gland - inactive At a slightly higher magnification the inactive tubules forming the lobules of the gland can be identified. Large areas of interlobular connective tissue, stained blue/green extend between these lobules. Within this connective tissue run the interlobular ducts. 250 µm

12. SLIDE 98 Mammary gland - inactive At a slightly higher magnification the inactive tubules forming the lobules of the gland can be identified. Large areas of interlobular connective tissue, stained blue/green extend between these lobules. Within this connective tissue run the interlobular ducts. 250 µm connective tissue interlobular duct lobule

13. SLIDE 98 Mammary gland - inactive The low columnar cells lining the walls of the tubules are mildly secretory - note the eosinophilic content of some of the tubules. 50 µm

14. SLIDE 98 Mammary gland - inactive The low columnar cells lining the walls of the tubules are mildly secretory - note the eosinophilic content of some of the tubules. 50 µm S : secretions in tubules S S S

15. SLIDE 98 Mammary gland - inactive How is the inactive mammary gland classified? 50 µm

16. SLIDE 98 Mammary gland - inactive How is the inactive mammary gland classified? 50 µm How is the inactive mammary gland classified? Compound tubular gland. interlobular connective tissue intralobular connective tissue blood vessel tubules

17. SLIDE 98 Mammary gland - inactive What are the elongated cells with nuclei lying at the base of the tubule epithelium? 25 µm

18. SLIDE 98 Mammary gland - inactive What are the elongated cells with nuclei lying at the base of the tubule epithelium? Myoepithelial cells. 25 µm T : tubules Arrowed : myoepithelial cells Ct : connective tissue (intralobular) S : secretion S Ct T T

19. SLIDE 98 Mammary gland - inactive Towards the teat the lactiferous ducts open into the lactiferous sinuses to teat sinus to teat canal. The double epithelial layers become progressively taller, from cuboidal to columnar. 0.5 mm

20. SLIDE 98 Mammary gland - inactive What is a distinguishing feature of the lactiferous ducts? 50 µm

21. SLIDE 98 Mammary gland - inactive What is a distinguishing feature of the lactiferous ducts? 50 µm What is a distinguishing feature of the lactiferous ducts? Double layered cuboidal to columnar epithelium. connective tissue lumen of duct double layered epithelium

22. Demonstration slide Mammary gland Transverse section through teat canal. Note : keratinized stratified squamous epithelium lining the canal, bands of smooth muscle and blood vessels.

23. Demonstration slide Mammary gland Transverse section through teat canal. Note : keratinized stratified squamous epithelium lining the canal, bands of smooth muscle and blood vessels. epithelium keratinised stratified squamous blood vessels smooth muscle

24. SLIDE 99 Mammary gland - active If you look at slide 99 in the class; start by examining the red/pink stained section. 0.5 mm

25. SLIDE 99 Mammary gland - active Note each lobule is greatly expanded; consisting mostly of alveoli. The interlobular connective tissue is compressed to fibrous tracts or seams. 250 µm

26. SLIDE 99 Mammary gland - active Note each lobule is greatly expanded; consisting mostly of alveoli. The interlobular connective tissue is compressed to fibrous tracts or seams. 250 µm adipocytes interlobular duct Ct : connective tissue A : alveoli Ct A A A

27. SLIDE 99 Mammary gland - active Identify : 1. lobule. 4. alveoli. 2. intralobular ducts. 5. interlobular ducts. 3 interlobular connective tissue. 250 µm

28. SLIDE 99 Mammary gland - active Identify : 1. lobule. 4. alveoli. 2. intralobular ducts. 5. interlobular ducts. 3 interlobular connective tissue. 250 µm 2 3 4 3 4 5 3 4 1

29. SLIDE 99 Mammary gland - active In the compressed seams of interlobular connective tissue, Identify : blood vessels. unilocular adipocytes. 100 µm

30. SLIDE 99 Mammary gland - active In the compressed seams of interlobular connective tissue, Identify : blood vessels. unilocular adipocytes. 100 µm arteriole unilocular adipocytes connective tissue (interlobular) alveoli

31. SLIDE 99 Mammary gland - active Most prominent in the interlobular connective tissue are the large interlobular and lactiferous ducts. 50 µm

32. SLIDE 99 Mammary gland - active Most prominent in the interlobular connective tissue are the large interlobular and lactiferous ducts. 50 µm interlobular duct note cuboidal/columnar epithelium interlobular connective tissue alveolus alveolar epithelium blood vessels

33. SLIDE 99 Mammary gland - active Within the lobules identify intralobular ducts. 50 µm

34. SLIDE 99 Mammary gland - active Within the lobules identify intralobular ducts. 50 µm Within the lobules identify intralobular ducts. Note the more regular epithelium of the duct. intralobular duct alveoli

35. SLIDE 99 Mammary gland - active How is the active mammary gland classified? 50 µm

36. SLIDE 99 Mammary gland - active How is the active mammary gland classified? 50 µm How is the active mammary gland classified? Compound tubulo-alveolar gland. alveoli connective tissue seams

37. SLIDE 99 Mammary gland - active Note the appearance of the alveoli : folded somewhat ‘ragged’ epithelium. content of fat droplets. eosinophilic protein containing secretions. 50 µm

38. SLIDE 99 Mammary gland - active Note the appearance of the alveoli : folded somewhat ‘ragged’ epithelium. content of fat droplets. eosinophilic protein containing secretions. 50 µm folded epithelium E : eosinophilic secretions E E adipocytes

39. SLIDE 99 Mammary gland - active Compare the staining of the large unilocular adipocytes in this section with their appearance in the lipid stained section which follows. 50 µm

40. SLIDE 99 Mammary gland - active Compare the staining of the large unilocular adipocytes in this section with their appearance in the lipid stained section which follows. When inactive; the alveolar cells are phagocytosed by invading macrophages. 50 µm unilocular adipocytes in seam of interlobular connective tissue alveolar epithelium

41. SLIDE 99 Mammary gland – active stained for lipid As seen before in histological sections; the presence of lipid can be demonstrated by the use of osmium containing solutions. In sections stained with H&E the unfixed lipid is lost during sample processing. 1.0 mm

42. SLIDE 99 Mammary gland – active stained for lipid As seen before in histological sections; the presence of lipid can be demonstrated by the use of osmium containing solutions. In sections stained with H&E the unfixed lipid is lost during sample processing. 1.0 mm All handling and use of osmium containing solutions must be carried out in a fume hood.

43. SLIDE 99 Mammary gland – active stained for lipid Compare the small globules of fat in the alveoli with the larger, darker globules of the adipose cells in the interlobular connective tissue. 250 µm

44. SLIDE 99 Mammary gland – active stained for lipid Compare the small globules of fat in the alveoli with the larger, darker globules of the adipose cells in the interlobular connective tissue. 250 µm Ua : unilocular adipocytes A A Ua A : alveolae interlobular connective tissue

45. SLIDE 99 Mammary gland – active stained for lipid When viewing this section examine under higher magnification, look at a brown/black area rather than intensely black over-stained areas. 100 µm

46. SLIDE 99 Mammary gland – active stained for lipid When viewing this section examine under higher magnification, look at a brown/black area rather than intensely black over-stained areas. 100 µm adipocytes interlobular connective tissue alveoli F : fat globules F F epithelium

47. SLIDE 99 Mammary gland – active stained for lipid Then at still higher magnification look for fat globules in the alveoli and small globules actually in the apical parts of the alveolar epithelial cells. 50 µm

48. SLIDE 99 Mammary gland – active stained for lipid Then at still higher magnification look for fat globules in the alveoli and small globules actually in the apical parts of the alveolar epithelial cells. 50 µm alveolar lumen free lipid droplets droplets in apical area of cell cell nucleus

49. SLIDE 99 Mammary gland – active stained for lipid Why would a stain dissolved in water stain fat sections. 50 µm

50. SLIDE 99 Mammary gland – active stained for lipid Why would a stain dissolved in water stain fat sections. 50 µm Why would a stain dissolved in water stain fat sections. The stain is more readily soluble in the fat than in water. lipid droplets in cell alveolar lumen

51. Slides 22, 23. Dr D.Kilroy. Second Year Histology. 025. Mammary. J.Bredl. 18.12.04. Gross Anatomy Correlates. Dr S.Frean. Histology Slides and Stains. Tanya Hopcroft. Compressed and updated. 22.08.2006. 2007.