1. Lecture 6 Outline (Ch. 9, 10) I.Respiration Recap II.Electron transport chain & oxidative phosphorylation (pp. 172-176) III.Anaerobic respiration (pp. 177-182) IV.Respiration with other molecules V.Chloroplasts VI.Light (pp. 187-193) VII. Before next lecture: Review slides and text, go over summary concept 9.4 and Q#10 on p. 184.

2. Steps of Respiration Glucose is OXIDIZED/REDUCED to _____. Oxygen is OXIDIZED/REDUCED to _____.

3. In thinking about cellular respiration: a. What is/are the overall function(s) of glycolysis? b. What is/are the overall function(s) of pyruvate oxidation and the Citric Acid (Krebs) cycle?

4. Steps of Respiration Stages of respiration: 4. ETC

5. Cellular Respiration ETC  e- collection molecules embedded on inner mitochondrial membrane Electron transport chain (ETC) accept e- in turn e- ultimately accepted by O 2 (O 2 reduced to H 2 O)

6. ~100 H+ (stored) 10 H 2 O -outputs: ATP (none yet) Where do outputs go? Electron transport chain (ETC) -inputs:per glucose, 10 NADH 2 FADH 2

7. Cellular Respiration 4. Chemiosmosis ATP synthase: inner mitochondrial membrane H+ stock-piled in inner membrane space = gradient chemiosmosis – ion gradient to do work

8. Cellular Respiration ATP synthase: enzyme that makes ATP using H+ gradient 4. Chemiosmosis H+ must enter matrix here Generates 1 ATP per ~3.4 H+

9. In thinking about cellular respiration: a. At what stage(s) in the overall process is each of the reactants used? b. At what stage(s) in the overall process is each of the products produced? C 6 H 12 O 6 + O 2  CO 2 + H 2 O + Energy

10. Cellular Respiration Summary of respiration KNOW THIS DIAGRAM – EXCELLENT SUMMARY

11. Coenzyme Q (or CoQ) is sold as a nutritional supplement, some say to “give you fuel”. Where in respiration is Coenzyme Q used? Does it “give you fuel”?

12. Cellular Respiration - anaerobic no O 2 – no oxidative phosphorylation fermentation = extension of glycolysis

13. Cellular Respiration - anaerobic Types of fermentation - 1. alcohol pyruvate converted to acetaldehyde acetaldehyde accepts e- to regenerate NAD+ ethanol produced brewing & baking

14. Cellular Respiration - anaerobic pyruvate accepts e- to regenerate NAD+ lactate produced Types of fermentation - 2. Lactic acid muscle fatigue & dairy industry

15. Cellular Respiration Comparison of aerobic vs. anaerobic respiration: ATP per glucose: AerobicAnaerobic initial e- acceptor: final e- acceptor:

16. Let’s say it takes 1,000 g of glucose to grow 10 g of yeast anaerobically. How many grams of glucose would it take (estimate approximately) to grow 10 g of yeast aerobically? Hint: If it takes X amount of glucose to grow 10 g of yeast without oxygen, what factor would you have to multiply or divide X by to grow 10 g of the same yeast with oxygen?

17. Cellular Respiration – other biomolecules Glucose catabolism – one option Proteins: Fats: enter CAC or before Catabolized into a.a. Amino group removed (pee out in urine) If have more glucose than needed, can run “backward” to store energy as glycogen or fats!

18. Self-Check Step of Respiration InputsOutputsCO 2 /H 2 OATP produced e- carriers loaded Glycolysis1 glucose2 pyruvateNone2 net2 NADH Coenzyme Junction Citric Acid Cycle Electron Transport Chain Oxidative phosphorylation & Chemiosmosis Fermentation

19. Photosynthesis - overview

20. Overall purpose: Photosynthesis - overview photosynthesis: light  chemical energy complements respiration - light reaction: solar energy harvest - Calvin cycle: energy to organics

21. chloroplast recap Outer membrane Inner membrane Thylakoid membrane Intermembrane space Stroma Thylakoid space

22. H 2 O + CO 2 + energy  C 6 H 12 O 6 + O 2 In photosynthesis, water is OXIDIZED/REDUCED to _______ and carbon dioxide is OXIDIZED/REDUCED to ______.

23. Redox Reactions Equation for photosynthesis CO 2 + H 2 O + light energy C 6 H 12 O 6 + O 2 photo synthesis

24. Photosynthesis – light absorption visible light ~380 to 750 nm chloroplast pigments – absorb blue-violet & red/orange - transmit and reflect green

25. pigments: chlorophyll a accessory pigments -energy-absorbing ring -hydrocarbon tail - carotenoids - photoprotective Photosynthesis – light absorption - chlorophyll b

26. chlorophyll a – abs blue-violet, red ~400-450, 650-700 chlorophyll b & carotenoids – abs broadly blue-violet 450-500 & 600-650 more wavelengths used for photosynthesis = more light energy absorbed Photosynthesis – light absorption

27. Self-Check