1. Supplementary Material Submitted to: Plant Growth Regulation Molecular characterization of an elicitor-responsive 3-hydroxy-3-methylglutaryl coenzyme A reductase gene involved in oleanolic acid production in cell cultures of Achyranthes bidentata Qiu Jun Wang 1 · Xiu Yun Lei 1 · Li Ping Zheng 2 · Jian Wen Wang 1,* 1 College of Pharmaceutical Sciences, Soochow University, Suzhou 215123, China 2 Department of Horticulture, School of Architecture, Soochow University, Suzhou 215123, China * JW Wang for Correspondence (e-mail: jwwang@suda.edu.cn; bcjwwang@gmail.com; Tel: +86 512 69561430; Fax: +86 512 65882089)jwwang@suda.edu.cnbcjwwang@gmail.com

2. Supplementary figures Legends to supplementary figures Fig. S1 HPLC analysis of oleanolic acid (OA) isolated from cultured cells of A. bidentata. The cell sample was taken 12 days after inoculation. The isolated OA was analyzed by HPLC employing Aglient HPLC system consisting of a diode array detector and Elite SinoChrom ODS-BP column (250 mm × 4.6mm) by UV detection at 210 nm. 10 µl samples were eluted with a mobile phase consisting of 90: 10 (v/v) methanol: water at a flow rate 1 mL/min. Fig. S2 Multiple alignment of AbHMGR with the following other plant HMGR: AtHMGR (XP_002887642), PkHMGR (ABC74565), DlHMGR (AET72044), LcHMGR (ABF56518), CsHMGR (AHB64333), MaHMGR (AAD03789) and MnHMGR (EXB56263). Similar residues are colored as the most conserved one (according to BLOSUM62, average BLOSUM62 score: Max: 3.0, Low: 0.5). Fig. S3 Phylogenic tree analyzes of HMGRs from various species. Accession numbers: Mus musculus, XP_127496; Oryctolagus cuniculus, Q29512; Dendroctonus jeffreyi, AAF80374; Ips paraconfusus, AAD20975; Saccharomyces cerevisiae, AAA34677; Zea mays, CAA70440; Oryza sativa, AAD38873; Achyranthes bidentata, KX462886; Camellia sinensis, AHB64333; Dimocarpus longan, AET72044; Litchi chinensis, ABF56518; Morus notabilis, EXB56263; M. alba, AAD03789; Picrorhiza kurrooa, ABC74565; Arabidopsis lyrata subsp. lyrata, XP_002887642; Nicotiana tabacum, AAL54878.

3. Fig. S1 HPLC analysis of oleanolic acid (OA) isolated from cultured cells of A. bidentata. The cell sample was taken 12 days after inoculation. The isolated OA was analyzed by HPLC employing Aglient HPLC system consisting of a diode array detector and Elite SinoChrom ODS-BP column (250 mm × 4.6mm) by UV detection at 210 nm. 10 µl samples were eluted with a mobile phase consisting of 90: 10 (v/v) methanol: water at a flow rate 1 mL/min

4. Fig. S2 Multiple alignment of AbHMGR with the following other plant HMGR: AtHMGR (XP_002887642), PkHMGR (ABC74565), DlHMGR (AET72044), LcHMGR (ABF56518), CsHMGR (AHB64333), MaHMGR (AAD03789) and MnHMGR (EXB56263). Similar residues are colored as the most conserved one (according to BLOSUM62, average BLOSUM62 score: Max: 3.0, Low: 0.5).

5. Fig. S3 Phylogenic tree analyzes of HMGRs from various species. Accession numbers: Mus musculus, XP_127496; Oryctolagus cuniculus, Q29512; Dendroctonus jeffreyi, AAF80374; Ips paraconfusus, AAD20975; Saccharomyces cerevisiae, AAA34677; Zea mays, CAA70440; Oryza sativa, AAD38873; Achyranthes bidentata, KX462886; Camellia sinensis, AHB64333; Dimocarpus longan, AET72044; Litchi chinensis, ABF56518; Morus notabilis, EXB56263; M. alba, AAD03789; Picrorhiza kurrooa, ABC74565; Arabidopsis lyrata subsp. lyrata, XP_002887642; Nicotiana tabacum, AAL54878.