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Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain.

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Presentation on theme: "Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain."— Presentation transcript:

1 Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain

2 Introduction Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain Kinetic range Thermodynamic range Extraction Distribution constant Temperature Ionic strength Analyte diffusivity Contact area Secondary reactions Time

3 Introduction Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain

4 Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain 20092010201120122013201420152016 Stir membrane extraction (SME) Stir membrane liquid extraction (SMLE) 2 phases 3 phases Application to Bioanalysis SMLE adapted to low sample volumes Stir membrane solid liquid liquid extraction Stir fabric phase extraction Stir C18 borosilicate disk Stir Nanohorns borosilicate disk Magnetically confined NPs Stir frit extraction

5 Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain 20092010201120122013201420152016 Stir membrane extraction (SME) Stir membrane liquid extraction (SMLE) 2 phases 3 phases Application to Bioanalysis SMLE adapted to low sample volumes Stir membrane solid liquid liquid extraction Stir fabric phase extraction

6 Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain 20092010201120122013201420152016 Stir membrane extraction (SME) Stir membrane liquid extraction (SMLE) 2 phases 3 phases Application to Bioanalysis SMLE adapted to low sample volumes Stir membrane solid liquid liquid extraction Stir fabric phase extraction

7 Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain 2009 Stir membrane extraction (SME) Membrane Assembly Device

8 Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain 2009 Stir membrane extraction (SME) Stir membrane (A) and the three different PTFE stir bar units, namely, (B) 622 mm 2 -surface PTFE stir bar, (C) 433 mm 2 -surface PTFE stir bar, and (D) 89 mm 2 -surface stir bar. (1) fluorene, (2) anthracene, (3) fluoranthene, (4) pyrene, and (5) benzo-anthracene.

9 Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain 20092010201120122013201420152016 Stir membrane extraction (SME) Stir membrane liquid extraction (SMLE) 2 phases 3 phases Application to Bioanalysis SMLE adapted to low sample volumes Stir membrane solid liquid liquid extraction Stir fabric phase extraction

10 Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain 20092010201120122013201420152016 Stir membrane extraction (SME) Stir membrane liquid extraction (SMLE) 2 phases 3 phases Application to Bioanalysis SMLE adapted to low sample volumes Stir membrane solid liquid liquid extraction Stir fabric phase extraction

11 Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain 2009 Stir membrane extraction (SME) 2016 Stir fabric phase extraction Fabric phase Device

12 Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain 2009 Stir membrane extraction (SME) 2016 Stir fabric phase extraction Fabric phase

13 Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain 20092010201120122013201420152016 Stir membrane extraction (SME) Stir membrane liquid extraction (SMLE) 2 phases 3 phases Application to Bioanalysis SMLE adapted to low sample volumes Stir membrane solid liquid liquid extraction Stir fabric phase extraction

14 Application to Bioanalysis Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain 20112012 Stir membrane liquid extraction (SMLE) (i) (ii) (iii) (v) (iv) (ii) (iii) Internal chamber Assembly Device

15 Application to Bioanalysis Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain 20112012 Stir membrane liquid extraction (SMLE) Extraction modes 2 phases 3 phases An organic solvent wetting the membrane and inside the acceptor chamber An organic solvent wetting the membrane and an aqueous solution inside the acceptor chamber. A pH gradient is the driving force for the transference of the analytes

16 Application to Bioanalysis Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain 20112012 Stir membrane liquid extraction (SMLE) Application to Bioanalysis Acidic sample HAA - + H + Alkaline acceptor phase NSAlDs

17 Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain 20092010201120122013201420152016 Stir membrane extraction (SME) Stir membrane liquid extraction (SMLE) 2 phases 3 phases Application to Bioanalysis SMLE adapted to low sample volumes Stir membrane solid liquid liquid extraction Stir fabric phase extraction

18 Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain 20092010201120122013201420152016 Stir membrane extraction (SME) Stir membrane liquid extraction (SMLE) 2 phases 3 phases Application to Bioanalysis SMLE adapted to low sample volumes Stir membrane solid liquid liquid extraction Stir fabric phase extraction

19 Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain 2013 SMLE adapted to low sample volumes Applicable to moderate to large sample volume Not useful for lower volumes than 10 mL pH gradient SLM Acceptor phase Sample 2 mL Parafilm Adaptation Agitated, not stirred Several samples at a time

20 Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain 20092010201120122013201420152016 Stir membrane extraction (SME) Stir membrane liquid extraction (SMLE) 2 phases 3 phases Application to Bioanalysis SMLE adapted to low sample volumes Stir membrane solid liquid liquid extraction Stir fabric phase extraction

21 Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain 20092010201120122013201420152016 Stir membrane extraction (SME) Stir membrane liquid extraction (SMLE) 2 phases 3 phases Application to Bioanalysis SMLE adapted to low sample volumes Stir membrane solid liquid liquid extraction Stir fabric phase extraction

22 Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain 2014 Stir membrane solid liquid liquid extraction Analyzing solid samples Sample Organic phase Acceptor solution Membrane Eppendorf SOLID-LIQUID extraction LIQUID-LIQUID extraction Methylparaben, ethylparaben, propylparaben, butylparaben and ethylparaben in lyophilized breast milk

23 Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain 20092010201120122013201420152016 Stir membrane extraction (SME) Stir membrane liquid extraction (SMLE) 2 phases 3 phases Application to Bioanalysis SMLE adapted to low sample volumes Stir membrane solid liquid liquid extraction Stir fabric phase extraction Stir C18 borosilicate disk Stir Nanohorns borosilicate disk Magnetically confined NPs Stir frit extraction

24 Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain 20092010201120122013201420152016 Stir C18 borosilicate disk Stir Nanohorns borosilicate disk Magnetically confined NPs Stir frit extraction

25 Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain 20092010201120122013201420152016 Stir C18 borosilicate disk Stir Nanohorns borosilicate disk Magnetically confined NPs Stir frit extraction

26 Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain 20132014 Stir C18 borosilicate disk Stir Nanohorns borosilicate disk C18 derivatization NHs immobilization

27 Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain 20092010201120122013201420152016 Stir C18 borosilicate disk Stir Nanohorns borosilicate disk Magnetically confined NPs Stir frit extraction

28 Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain 20092010201120122013201420152016 Stir C18 borosilicate disk Stir Nanohorns borosilicate disk Magnetically confined NPs Stir frit extraction

29 Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain 20122013 Magnetically confined NPs Stir frit extraction MNPs Magnet Commercial polyethylene frit Iron wire SPE 3 mL SorbentFrit

30 Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain 20092010201120122013201420152016 Stir membrane extraction (SME) Stir membrane liquid extraction (SMLE) 2 phases 3 phases Application to Bioanalysis SMLE adapted to low sample volumes Stir membrane solid liquid liquid extraction Stir fabric phase extraction Stir C18 borosilicate disk Stir Nanohorns borosilicate disk Magnetically confined NPs Stir frit extraction

31 Integrating extraction and stirring in microextraction techniques R. Lucena Analytical Chemistry Department, University of Córdoba, Spain

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