1. By Kavitha kannan, SBST

2. Liver is the most important and large glandular organ in human body that necessary for survival. The main functions are detoxification, protein synthesis, and decomposition of red blood cell and production of biochemical necessary for digestion such as bile and hormone. Liver diseases or liver failures are any disturbance of liver function that causes illness. Jaundice is yellow discoloration of skin, mucous membrane and sclera due to yellow pigmentation of plasma is caused by accumulation of bilirubin. INTRODUCTION

3. Cholestatic is not a disease; rather, it is a symptom of many diseases. It is defined as a pathologic state of reduced bile formation or flow of bile from the liver and duodenum. In the absence of a reliable liver protective drug in modern medicine there are a number of medicinal preparations in Ayurveda recommended for the treatment of liver disorders. Eclipta prostrata is considered a primary liver herb in Ayurveda. A single drug cannot be effective for all types of severe liver diseases. Therefore an effective formulation has to be developed using indigenous medicinal plants.

4. METHODOLOGY For In vivo studies, 25 male Wistar albino rats are weighed 150 – 200 g were used. The male Wistar rats were put on adaptation basis to the laboratory condition for two weeks prior to experiment. The Wistar rats were kept in separate cage in the animal research room with controlled temperature (22ºC ± 3) and in a 12 hour light or dark cycle with lights on till 7.00 a.m. Standard commercial pellet diet was given twice a day and water ad libitum.

5. Data analysis using SPSS 20.0 and Anova Test Determination of AST, ALT and Total Bilirubin level in blood Group 5 (5 rats) Rats fed with normal diet with single dose of Norfloxacin plus 350mg/kg of Eclipta prostrata. Group 4 (5 rats) Rats fed with normal diet with single dose of Norfloxacin plus 250mg/kg of Eclipta prostrata. Group 3 (5 rats) Rats fed with normal diet with single dose of Norfloxacin plus 150mg/kg of Eclipta prostrata. Group 2 (5 rats) Rats fed with normal diet with single dose of Norfloxacin without Eclipta prostrata. Group 1 (5 rats) Rats fed with normal diet Selection of sample 25 Male Rats

6. EXTRACTION PREPARATION Eclipta prostrata leaves were collected from rural areas and housing areas around the place. The leaves then were washed thoroughly with tap water. Once washed, the leaves were dried under shaded area for seven days followed by final drying of leaves in the oven at 50°C for 4 to 6 hours. The dried leaves were ground to a very fine powder by using an electrical grinder. After grinded, 100g of Eclipta prostrata crude leaf powder were soaked in 1000ml of distilled water and left to stand overnight on electrical stirrer. On the next day, the mixture was collected and filtered through muslin cloth in order to obtain the extract. The extract then was evaporated by using water bath system and rotary evaporator at 40 - 45 °C for 2 days. Finally, the Eclipta prostrata aqueous leaves extract was weighed on an analytical balance and mixed with 1ml of distilled water to prepare various dosage of concentration.

7. DRUG PREPARATION 400mg of Norfloxacin was dissolved in 10 ml of deionized water at 70°C. The mixture was well mixed by using a glass rod. The prepared Norfloxacin was stored in test tube and kept refrigerated.

8. RESULT Aspartate Transaminase Treatment GroupMean ± Standard Deviation Group 1 (Negative Control)62.22 ± 10.48 Group 2 (Positive Control)213.56 ± 5.81 Group 3 (Norfloxacin + 150mg/kg EP)159.38 ± 22.87 Group 4 (Norfloxacin + 250 mg/kg EP)97.10 ± 9.13 Group 5 (Norfloxacin + 350 mg/kg EP)71.66 ± 10.10 Mean values of serum AST level in normal and experimental rats.

9. Alanine Transaminase Treatment GroupMean ± Standard Deviation Group 1 (Negative Control)25.78 ± 2.4 Group 2 (Positive Control)66.54 ± 2.09 Group 3 (Norfloxacin + 150mg/kg EP)53.52 ± 1.03 Group 4 (Norfloxacin + 250 mg/kg EP)38.78 ± 5.15 Group 5 (Norfloxacin + 350 mg/kg EP)27.21 ± 2.69 Mean values of serum ALT level in normal and experimental rats Total Bilirubin Treatment GroupMean ± Standard Deviation Group 1 (Negative Control)0.40 ± 0.11 Group 2 (Positive Control)2.52 ± 0.56 Group 3 (Norfloxacin + 150mg/kg EP)1.25 ± 0.19 Group 4 (Norfloxacin + 250 mg/kg EP)0.69 ± 0.03 Group 5 (Norfloxacin + 350 mg/kg EP)0.51 ± 0.04 Mean values of serum TB level in normal and experimental rats.

10. Measurement of AST Levels in Norfloxacin induced rats and Eclipta prostrata treated groups

11. Measurement of ALT Levels in Norfloxacin induced rats and Eclipta prostrata treated groups

12. Measurement of TB Levels in Norfloxacin induced rats and Eclipta prostrata treated groups

13. In conclusion, the observations from the current study show that the aqueous leaf extract of Eclipta Prostrata (EP) lowered the AST, ALT, Total Bilirubin level significantly in Cholestatic Jaundice rats. There is also improvement in symptoms of jaundice observed in treated groups and Norfloxacin induced rats which were treated with various dosages of Eclipta Prostrata extract administrations such as 150 mg/kg, 250 mg/kg and 350 mg/kg. It had shown the symptoms reduced significantly at higher doses of aqueous Eclipta Prostrata extract administration. The compounds that present in Eclipta Prostrata mainly the flavonoid, has been used as protective agent against Cholestatic Jaundice and worked to reduce the liver marker enzymes AST, ALT and also Total Bilirubin.