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Tissue engineering of aortic tissue: dire consequence of suboptimal elastic fiber synthesis in vivo by Florian Opitz, Katja Schenke-Layland, Tina U Cohnert,

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Presentation on theme: "Tissue engineering of aortic tissue: dire consequence of suboptimal elastic fiber synthesis in vivo by Florian Opitz, Katja Schenke-Layland, Tina U Cohnert,"— Presentation transcript:

1 Tissue engineering of aortic tissue: dire consequence of suboptimal elastic fiber synthesis in vivo by Florian Opitz, Katja Schenke-Layland, Tina U Cohnert, Barry Starcher, Karl Juergen Halbhuber, David P Martin, and Ulrich A Stock Cardiovasc Res Volume 63(4):719-730 September 1, 2004 Copyright © 2004, European Society of Cardiology

2 Macroscopical appearance of TE blood vessel grafts prior to implantation and 1, 3, 6, 12 and 24 weeks after implantation. Florian Opitz et al. Cardiovasc Res 2004;63:719-730 Copyright © 2004, European Society of Cardiology

3 Scanning electron micrograph of the luminal surface from native aorta (A) and TEBV cultured for 6 weeks in vivo. Florian Opitz et al. Cardiovasc Res 2004;63:719-730 Copyright © 2004, European Society of Cardiology

4 H&E stains of native aorta and TEBV 1,3,6, 12 and 24 weeks after implantation. Florian Opitz et al. Cardiovasc Res 2004;63:719-730 Copyright © 2004, European Society of Cardiology

5 Immunostaining of native aorta and grafts cultured for 12 weeks. Florian Opitz et al. Cardiovasc Res 2004;63:719-730 Copyright © 2004, European Society of Cardiology

6 Resorcin-Fuchsin and Harts staining of native aorta compared to TEBV 12 weeks after implantation (A), van Kossa staining of native aorta and TEBV 24 weeks after implantation (B), Movat Pentachrome staining of native aorta compared to TEBV 6 weeks after implantation (C). Florian Opitz et al. Cardiovasc Res 2004;63:719-730 Copyright © 2004, European Society of Cardiology

7 Desmosine content (pmol/mg protein) in TEBVsTEBV compared to native aorta. Florian Opitz et al. Cardiovasc Res 2004;63:719-730 Copyright © 2004, European Society of Cardiology

8 (A) Transmission electron micrographs from native aorta and TEBV cultured for 6 weeks. Florian Opitz et al. Cardiovasc Res 2004;63:719-730 Copyright © 2004, European Society of Cardiology

9 Two-photon autofluorescence overlay of images of native (A, B, C) and TE (D, E, F) grafts, induced with 760 nm (green: elastic fibers) and 840 nm (red: collagen) laser pulses. Florian Opitz et al. Cardiovasc Res 2004;63:719-730 Copyright © 2004, European Society of Cardiology

10 Immunostaining of native aorta and TEBV against alpha smooth muscle actin (A), smooth muscle myosin (B) and desmin (C). Florian Opitz et al. Cardiovasc Res 2004;63:719-730 Copyright © 2004, European Society of Cardiology

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