1. Figure S1additional MS/MS Spectra and b, y ion tables of Phosphorylated Peptides in KO and WT samples, whose Debunker or A score is lower than the cut-off value. ( Δ Indicate to phosphorylated residue)

2. y 3 -H 3 PO 4 y3y3 y 4 -H 3 PO 4 y 6 -H 3 PO 4 MH-H 3 PO 4 3+ y6y6 b6b6 y 9 -H 3 PO 4 b7b7 MH-H 3 PO 4 2+ b8b8 y 11 -H 3 PO 4 y 11 y 12 y 12 -H 3 PO 4 b 15 b 16 -H 3 PO 4 y 13 -H 2 O y 14 b 16 b 17 +H 2 O 7 SSWWMLNPEGGKSGKS Δ PR [M+2H] 2+ m/z: 1042.98

3. b 3 -H 2 O b4b4 y5y5 y 15 2+ y7y7 y 14 2+ y 7 -H 3 PO 4 y8y8 y 9 -H 3 PO 4 MH-H 3 PO 4 2+ b 11 b 12 -H 2 O b 12 b 13 b 14 -H 3 PO 4 y 13 y 14 y 15 y 15 -H 2 O b 17 -H 3 PO 4 y 17 b 17 y 18 YSFAPPNTSLNSPS Δ PNYSK [M+2H] 2+ m/z: 1076.48 10

4. y 4 2+ y3y3 y 5 -H 3 PO 4 2+ y 6 -H 3 PO 4 2+ b 11 3+ b4b4 b2b2 b 18 4+ b 15 3+ b 11 2+ b6b6 y 13 2+ y 19 3+ y 14 -H 2 O 2+ b8b8 y 8 -H 3 PO 4 y8y8 b 12 -H 2 O TLPHVVNTMPHTSAMNRLT Δ PVK11 [M+4H] 4+ m/z: 631.82