1. What Is the Amgen Biotech Experience?

3. Why should your students study biotechnology?
Why Biotech?

4. Basics of ABE Amgen Biotech Experience Student and teacher curriculum
Basic content, lab techniques, and skills used by researchers in the biotechnology field
Choice of lab sequences
All specialized equipment provided including consumables at no cost
Teachers provide limited, commonplace equipment (beakers, etc)
Basics of ABE

5. Problem: How can we mass produce a useful protein
Problem: How can we mass produce a useful protein? Solution: Take the protein from the organism that makes it and put it into something that will make large quantities of that protein.
What are they doing?

6. How do they do it? Procedure Find the gene that makes the protein
Cut the gene out
Put the gene into a carrier
Use the carrier to insert the gene into bacteria
Turn on the gene so the bacteria makes the protein
How do they do it?

7. Student Guide
Diabetes and insulin production is used as an example throughout the curriculum
Learning goals
Visuals
Literacy strategies
Relevant analysis questions
Glossaries

8. Choice of lab sequences
Teacher’s Guide
Choice of lab sequences
Detailed teacher prep information with materials lists, step-by-step instructions, and prep tips
Identified media resources specific to an activity (on web-site)
Approximate timing is given for all activities
Supplemental activities support each lab
During the lab, specific questions encourage student discourse and reflection
Lab safety information for student and teacher

9. Procedures Involved Red Fluorescent Protein
Restriction (cut the rfp gene out)
Ligation (put the rfp gene into a plasmid)
Confirmation
Transformation (put the plasmid with rfp into a bacteria)
Culture the bacteria, isolate and purify the protein
Procedures Involved

10. Lab Sequences Complete GE Abridged GE Focus on Bacteria
Intro to Biotech
1. Practice Pipetting X
2. Restriction Analysis
3. Ligation
4. Confirmation
5. Transformation
6. Culture of E. coli/Purification *
X = included; * = optional
Lab Sequences

11. October 12, 2013 Saturday Introduction What are the ABE labs?
Lab 1.1 & 1.2: How To Use a Micropipette and Gel Electrophoresis
Lunch
Lab 5A: Transforming Bacteria with the pARA-R Plasmid
October 12, 2013

12. Sunday
Lab 2a: Preparing to Verify the RFP Gene: Digesting the pARA-R Plasmid
Lab 4a: Verification of the Recombinant Plasmid Using Gel Electrophoresis
Lunch
Lab 4a: Verification of the Recombinant Plasmid Using Gel Electrophoresis (cont.)
Lab 5A: Transforming Bacteria with the pARA-R Plasmid (cont.)
October 13, 2013

13. Procedures Involved Red Fluorescent Protein
Restriction (cut the rfp gene out)
Ligation (put the rfp gene into a plasmid)
Confirmation
Transformation (put the plasmid with rfp into a bacteria)
Culture the bacteria, isolate and purify the protein
Procedures Involved

14. Lab Sequences Complete GE Abridged GE Focus on Bacteria
Intro to Biotech
1. Practice Pipetting X
2. Restriction Analysis
3. Ligation
4. Confirmation
5. Transformation
6. Culture of E. coli/Purification *
X = included; * = optional
Lab Sequences