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Chapter 47 Animal Development
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Overview: A Body-Building Plan
It is difficult to imagine that each of us began life as a single cell called a zygote A human embryo at about 6–8 weeks after conception shows development of distinctive features
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Fig. 47-1 Figure 47.1 How did this complex embryo form from a single cell? 1 mm
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The question of how a zygote becomes an animal has been asked for centuries
As recently as the 18th century, the prevailing theory was called preformation Preformation is the idea that the egg or sperm contains a miniature infant, or “homunculus,” which becomes larger during development
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Fig. 47-2 Figure 47.2 A “homunculus” inside the head of a human sperm
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Development is determined by the zygote’s genome and molecules in the egg called cytoplasmic determinants Cell differentiation is the specialization of cells in structure and function Morphogenesis is the process by which an animal takes shape
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Model organisms are species that are representative of a larger group and easily studied, for example, Drosophila and Caenorhabditis elegans Classic embryological studies have focused on the sea urchin, frog, chick, and the nematode C. elegans
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Concept 47.1: After fertilization, embryonic development proceeds through cleavage, gastrulation, and organogenesis Important events regulating development occur during fertilization and the three stages that build the animal’s body Cleavage: cell division creates a hollow ball of cells called a blastula Gastrulation: cells are rearranged into a three-layered gastrula Organogenesis: the three layers interact and move to give rise to organs
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Fertilization Fertilization brings the haploid nuclei of sperm and egg together, forming a diploid zygote The sperm’s contact with the egg’s surface initiates metabolic reactions in the egg that trigger the onset of embryonic development
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The Acrosomal Reaction
The acrosomal reaction is triggered when the sperm meets the egg The acrosome at the tip of the sperm releases hydrolytic enzymes that digest material surrounding the egg
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Fig Basal body (centriole) Sperm head Figure 47.3 The acrosomal and cortical reactions during sea urchin fertilization For the Cell Biology Video Cortical Granule Fusion Following Egg Fertilization, go to Animation and Video Files. Acrosome Jelly coat Vitelline layer Sperm-binding receptors Egg plasma membrane
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Fig Basal body (centriole) Sperm head Figure 47.3 The acrosomal and cortical reactions during sea urchin fertilization Acrosome Hydrolytic enzymes Jelly coat Vitelline layer Sperm-binding receptors Egg plasma membrane
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Fig Sperm nucleus Acrosomal process Basal body (centriole) Actin filament Sperm head Figure 47.3 The acrosomal and cortical reactions during sea urchin fertilization Acrosome Hydrolytic enzymes Jelly coat Vitelline layer Sperm-binding receptors Egg plasma membrane
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Fig Sperm plasma membrane Sperm nucleus Acrosomal process Basal body (centriole) Actin filament Sperm head Fused plasma membranes Figure 47.3 The acrosomal and cortical reactions during sea urchin fertilization Acrosome Hydrolytic enzymes Jelly coat Vitelline layer Sperm-binding receptors Egg plasma membrane
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Fig Sperm plasma membrane Sperm nucleus Fertilization envelope Acrosomal process Basal body (centriole) Actin filament Sperm head Cortical granule Fused plasma membranes Figure 47.3 The acrosomal and cortical reactions during sea urchin fertilization Perivitelline space Acrosome Hydrolytic enzymes Jelly coat Vitelline layer Sperm-binding receptors Egg plasma membrane EGG CYTOPLASM
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Gamete contact and/or fusion depolarizes the egg cell membrane and sets up a fast block to polyspermy
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The Cortical Reaction Fusion of egg and sperm also initiates the cortical reaction This reaction induces a rise in Ca2+ that stimulates cortical granules to release their contents outside the egg These changes cause formation of a fertilization envelope that functions as a slow block to polyspermy
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Fig. 47-4 EXPERIMENT 10 sec after fertilization 25 sec 35 sec 1 min 500 µm RESULTS 1 sec before fertilization 10 sec after fertilization 20 sec 30 sec 500 µm Figure 47.4 Is the distribution of Ca2+ in an egg correlated with formation of the fertilization envelope? For the Cell Biology Video Calcium Release Following Egg Fertilization, go to Animation and Video Files. For the Cell Biology Video Calcium Wave Propagation in Fish Eggs, go to Animation and Video Files. CONCLUSION Point of sperm nucleus entry Spreading wave of Ca2+ Fertilization envelope
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10 sec after fertilization 25 sec 35 sec 1 min 500 µm
Fig. 47-4a EXPERIMENT Figure 47.4 Is the distribution of Ca2+ in an egg correlated with formation of the fertilization envelope? 10 sec after fertilization 25 sec 35 sec 1 min 500 µm
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1 sec before fertilization 10 sec after fertilization 20 sec 30 sec
Fig. 47-4b RESULTS 1 sec before fertilization Figure 47.4 Is the distribution of Ca2+ in an egg correlated with formation of the fertilization envelope? 10 sec after fertilization 20 sec 30 sec 500 µm
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CONCLUSION Point of sperm nucleus entry Spreading wave of Ca2+
Fig. 47-4c CONCLUSION Point of sperm nucleus entry Spreading wave of Ca2+ Fertilization envelope Figure 47.4 Is the distribution of Ca2+ in an egg correlated with formation of the fertilization envelope?
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Activation of the Egg The sharp rise in Ca2+ in the egg’s cytosol increases the rates of cellular respiration and protein synthesis by the egg cell With these rapid changes in metabolism, the egg is said to be activated The sperm nucleus merges with the egg nucleus and cell division begins
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Fertilization in Mammals
Fertilization in mammals and other terrestrial animals is internal In mammalian fertilization, the cortical reaction modifies the zona pellucida, the extracellular matrix of the egg, as a slow block to polyspermy
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Zona pellucida Follicle cell Sperm nucleus Cortical granules
Fig. 47-5 Zona pellucida Follicle cell Figure 47.5 Fertilization in mammals Sperm nucleus Cortical granules Sperm basal body
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In mammals the first cell division occurs 12–36 hours after sperm binding
The diploid nucleus forms after this first division of the zygote
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Cleavage Fertilization is followed by cleavage, a period of rapid cell division without growth Cleavage partitions the cytoplasm of one large cell into many smaller cells called blastomeres The blastula is a ball of cells with a fluid-filled cavity called a blastocoel
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(a) Fertilized egg (b) Four-cell stage (c) Early blastula
Fig. 47-6 (a) Fertilized egg Figure 47.6 Cleavage in an echinoderm embryo (b) Four-cell stage (c) Early blastula (d) Later blastula
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(a) Fertilized egg Fig. 47-6a
Figure 47.6 Cleavage in an echinoderm embryo (a) Fertilized egg
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(b) Four-cell stage Fig. 47-6b
Figure 47.6 Cleavage in an echinoderm embryo (b) Four-cell stage
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(c) Early blastula Fig. 47-6c
Figure 47.6 Cleavage in an echinoderm embryo (c) Early blastula
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(d) Later blastula Fig. 47-6d
Figure 47.6 Cleavage in an echinoderm embryo (d) Later blastula
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The eggs and zygotes of many animals, except mammals, have a definite polarity
The polarity is defined by distribution of yolk (stored nutrients) The vegetal pole has more yolk; the animal pole has less yolk
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The three body axes are established by the egg’s polarity and by a cortical rotation following binding of the sperm Cortical rotation exposes a gray crescent opposite to the point of sperm entry
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(a) The three axes of the fully developed embryo
Fig. 47-7 Dorsal Right Anterior Posterior Left Ventral (a) The three axes of the fully developed embryo Animal pole Pigmented cortex First cleavage Animal hemisphere Point of sperm nucleus entry Figure 47.7 The body axes and their establishment in an amphibian Future dorsal side Vegetal hemisphere Gray crescent Vegetal pole (b) Establishing the axes
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(a) The three axes of the fully developed embryo
Fig. 47-7a Dorsal Right Anterior Posterior Left Ventral Figure 47.7 The body axes and their establishment in an amphibian (a) The three axes of the fully developed embryo
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(b) Establishing the axes
Fig. 47-7b-1 Animal pole Animal hemisphere Vegetal hemisphere Figure 47.7 The body axes and their establishment in an amphibian Vegetal pole (b) Establishing the axes
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Point of sperm nucleus entry
Fig. 47-7b-2 Pigmented cortex Point of sperm nucleus entry Future dorsal side Gray crescent Figure 47.7 The body axes and their establishment in an amphibian (b) Establishing the axes
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(b) Establishing the axes
Fig. 47-7b-3 First cleavage Figure 47.7 The body axes and their establishment in an amphibian (b) Establishing the axes
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Point of sperm nucleus entry Animal hemisphere
Fig. 47-7b-4 Animal pole First cleavage Pigmented cortex Point of sperm nucleus entry Animal hemisphere Future dorsal side Vegetal hemisphere Gray crescent Vegetal pole Figure 47.7 The body axes and their establishment in an amphibian (b) Establishing the axes
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Cleavage planes usually follow a pattern that is relative to the zygote’s animal and vegetal poles
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Zygote Fig. 47-8-1 Figure 47.8 Cleavage in a frog embryo
For the Cell Biology Video Cleavage of a Fertilized Egg, go to Animation and Video Files.
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Fig 2-cell stage forming Figure 47.8 Cleavage in a frog embryo
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Fig 4-cell stage forming Figure 47.8 Cleavage in a frog embryo
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Animal pole 8-cell stage Vegetal pole Fig. 47-8-4
Figure 47.8 Cleavage in a frog embryo
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Blastula (cross section)
Fig Blastocoel Blastula (cross section) Figure 47.8 Cleavage in a frog embryo
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Blastula (cross section)
Fig 0.25 mm 0.25 mm Animal pole Blastocoel Figure 47.8 Cleavage in a frog embryo Vegetal pole Zygote 2-cell stage forming 4-cell stage forming 8-cell stage Blastula (cross section)
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Cell division is slowed by yolk
Holoblastic cleavage, complete division of the egg, occurs in species whose eggs have little or moderate amounts of yolk, such as sea urchins and frogs
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Meroblastic cleavage, incomplete division of the egg, occurs in species with yolk-rich eggs, such as reptiles and birds
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Gastrulation Gastrulation rearranges the cells of a blastula into a three-layered embryo, called a gastrula, which has a primitive gut
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Video: Sea Urchin Embryonic Development
The three layers produced by gastrulation are called embryonic germ layers The ectoderm forms the outer layer The endoderm lines the digestive tract The mesoderm partly fills the space between the endoderm and ectoderm Video: Sea Urchin Embryonic Development
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Gastrulation in the sea urchin embryo
The blastula consists of a single layer of cells surrounding the blastocoel Mesenchyme cells migrate from the vegetal pole into the blastocoel The vegetal plate forms from the remaining cells of the vegetal pole and buckles inward through invagination
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Gastrulation in the sea urchin embryo
The newly formed cavity is called the archenteron This opens through the blastopore, which will become the anus
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Future ectoderm Future mesoderm Future endoderm Animal pole Blastocoel
Fig Future ectoderm Future mesoderm Future endoderm Animal pole Blastocoel Mesenchyme cells Figure 47.9 Gastrulation in a sea urchin embryo Vegetal plate Vegetal pole
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Future ectoderm Future mesoderm Future endoderm Fig. 47-9-2
Figure 47.9 Gastrulation in a sea urchin embryo
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Filopodia pulling archenteron tip
Fig Future ectoderm Future mesoderm Future endoderm Filopodia pulling archenteron tip Figure 47.9 Gastrulation in a sea urchin embryo Archenteron
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Future ectoderm Future mesoderm Future endoderm Blastocoel Archenteron
Fig Future ectoderm Future mesoderm Future endoderm Blastocoel Figure 47.9 Gastrulation in a sea urchin embryo Archenteron Blastopore
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Mesenchyme (mesoderm forms future skeleton)
Fig Future ectoderm Future mesoderm Future endoderm Ectoderm Mouth Figure 47.9 Gastrulation in a sea urchin embryo Mesenchyme (mesoderm forms future skeleton) Digestive tube (endoderm) Anus (from blastopore)
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Figure 47.9 Gastrulation in a sea urchin embryo
Key Future ectoderm Future mesoderm Future endoderm Archenteron Blastocoel Filopodia pulling archenteron tip Animal pole Blastocoel Archenteron Blastocoel Blastopore Mesenchyme cells Ectoderm Vegetal plate Vegetal pole Mouth Figure 47.9 Gastrulation in a sea urchin embryo Mesenchyme cells Mesenchyme (mesoderm forms future skeleton) Digestive tube (endoderm) Blastopore 50 µm Anus (from blastopore)
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Gastrulation in the frog
The frog blastula is many cell layers thick Cells of the dorsal lip originate in the gray crescent and invaginate to create the archenteron Cells continue to move from the embryo surface into the embryo by involution These cells become the endoderm and mesoderm
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Gastrulation in the frog
The blastopore encircles a yolk plug when gastrulation is completed The surface of the embryo is now ectoderm, the innermost layer is endoderm, and the middle layer is mesoderm
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Fig SURFACE VIEW CROSS SECTION Animal pole Blastocoel Dorsal lip of blasto- pore Dorsal lip of blastopore Key Blastopore Future ectoderm Figure Gastrulation in a frog embryo Early gastrula Future mesoderm Vegetal pole Future endoderm
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Fig SURFACE VIEW CROSS SECTION Blastocoel shrinking Archenteron Key Future ectoderm Figure Gastrulation in a frog embryo Future mesoderm Future endoderm
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Fig SURFACE VIEW CROSS SECTION Ectoderm Mesoderm Blastocoel remnant Endoderm Archenteron Key Blastopore Future ectoderm Figure Gastrulation in a frog embryo Late gastrula Future mesoderm Blastopore Yolk plug Future endoderm
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Figure 47.10 Gastrulation in a frog embryo
SURFACE VIEW CROSS SECTION Animal pole Blastocoel Dorsal lip of blasto- pore Dorsal lip of blastopore Blastopore Early gastrula Vegetal pole Blastocoel shrinking Archenteron Figure Gastrulation in a frog embryo Ectoderm Mesoderm Blastocoel remnant Endoderm Archenteron Key Blastopore Future ectoderm Future mesoderm Late gastrula Blastopore Yolk plug Future endoderm
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Gastrulation in the chick
The embryo forms from a blastoderm and sits on top of a large yolk mass During gastrulation, the upper layer of the blastoderm (epiblast) moves toward the midline of the blastoderm and then into the embryo toward the yolk
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The midline thickens and is called the primitive streak
The movement of different epiblast cells gives rise to the endoderm, mesoderm, and ectoderm
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Migrating cells (mesoderm) Hypoblast
Fig Dorsal Fertilized egg Primitive streak Anterior Embryo Left Right Yolk Posterior Ventral Primitive streak Epiblast Future ectoderm Figure Gastrulation in a chick embryo Blastocoel Endoderm Migrating cells (mesoderm) Hypoblast YOLK
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