1. From Chemistry to bioinformatics
Systems Biology for Translational Medicine
Prospective Project Partnership Meeting
From Chemistry to bioinformatics
Osman Doluca
Department of Biomedical Engineering

2. Field of Study / Technical Experience
Keywords:
Structural biology:
fluorescence/FRET, CD spectrometry, Mass spec., NMR
Chemical modifications of oligonucleotides:
chemical synthesis of DNA/RNA primers
Bioinformatics:
programming using Python
phylogenetic analysis
prediction of motifs on the genome
Proteins:
site-directed mutagenesis of proteins
10 L scale bioreactor processing
enzyme production and characterization

3. Projects PhD study:an oligonucleotide story
1. controlling stability of G-quadruplex DNA structures using DNA modification

4. Projects PhD study:an oligonucleotide story
2. synthesis of triple helical DNA forming probes

5. Projects PhD study: an oligonucleotide story
3. AFENA (Assembly-dependent Fluorescently Enhancing Nucleic Acid)

6. Projects
PhD study: an oligonucleotide story

7. Projects Bioinformatics:
Prediction of G-quadruplex structures using genomic data

8. Projects Bioinformatics:
Prediction of G-quadruplex structures using genomic data

9. Ongoing Projects Bioinformatics:
P53 gene has 20 copies in long-living, low cancer prevalence elephants while humans have only 1. Is this a evolutionary response to big body size? How can we investigate relationship of copy numbers of other genes vs cancer rate, longevity and body size?
pyCOPP, Python Counter for Orthologous and Paralogous Proteins

10. Project Idea / Contribution Fields
List of the fields you may participate and/or techniques you may offer:
Oligonucleotide synthesis / Primer synthesis;
Design and synthesis of Fluorescent DNA probes for mRNA/miRNA/genomic DNA etc. for FISH/qPCR;
Structural analysis of biomolecules using CD-spectro, UV melting, Mass spectro, NMR, FTIR;
Protein engineering and production; enzyme characterization
Python programming for automated genome analysis

11. Contact Details Osman Doluca Department of Biomedical Engineering
homes.ieu.edu.tr/odoluca/