1. Aquaporins: Investigating the Structure and Function Cell Membrane Water Channels

2. Interdisciplinary Investigations (IDI)
Chemistry 161
Chemistry 162
Chemistry 163
Epigenetics
Aquaporins
Day 1
Pre-Lab Discussion
Part 1: Aquaporins
Part 2: IDI Challenge 1
Day 2
Part 3: Seminar
Part 4: IDI Challenge 2
Part 5: Post-lab Assignment

3. What did you find most interesting about each video?
Pre-Lab Discussion
What did you find most interesting about each video?

4. The Fluid Mosaic Model of the Cell Membrane

5. Key Structural Component: The Phospholipid
Think/ Pair/ Share: What do you notice about the structure of this molecule?
Think/ Pair/ Share: What does this mean for the intermolecular forces between these molecules?

6. Key Structural Component: The Phospholipid

7. Key Structural Component: A Mosaic of Proteins

8. A Quick Look at Protein Structure

9. A Quick Look at Protein Structure

10. Key Features of Membrane Proteins
Think/ Pair/ Share: What must be true about the structure of proteins that span the cell membrane?

11. Visualizing the Cell Membrane:

12. Crossing the Cell Membrane
What types of things does a cell need to get across its membrane?
Of those things, which can cross freely?? Why?? What about everything else??

13. X Cell membranes are SELECTIVELY PERMEABLE
O2, CO2, and other small, nonpolar molecules; and H2O
large, polar molecules; ions
such as H+, Na+, CI-, Ca++; X

14. Part 1 B: Intermolecular Forces & Cell Membranes

16. Part 1 B: Intermolecular Forces & Cell Membranes

17. C. Hydrogen Bonding

18. D. Amino Acid ~ Arginine

19. Part 2: IDI Challenge
On your white board, make a group drawing that represents your understanding of protein structure and intermolecular forces to construct a model (drawing) of what a water channel in a cell membrane would look like, and how it would orient itself in a plasma membrane.
Would such a channel need to be specific for water? If so, how could it accomplish this specificity?
Be sure to capture your drawing as a photograph for your post-lab report!

20. Part 3: Seminar Assignment For Next Week: Read these two articles:
“Precious Bodily Fluids”, Pittsburgh Supercomputing Center, 2002
Another article about water channels??
2. Write a summary of the articles.
3. Write three questions
4. Bring the summary and questions with you to class and be prepared to participate in an in-class seminar

21. Day 2 Agenda
Your Drawings…
Hydrogen Bonds!
Seminar
Challenge #2

22. Group 1

23. Group 2

24. Group 3

25. Group 4

26. Group 5

28. Hydrogen Bonding Can you recognize them now?
Draw an H-bond between two molecules of the following if possible:
a. CH3NH2
b. CH3N(CH3)2

29. Part 3: Seminar
Find your team!
Introductions and Roles.
Discuss the papers, using your questions as a guide.
Report out!

30. Aquaporin

31. Amino Acid ~ Arginine

32. Amino Acid: Asparagine

33. Day 2: IDI Challenge
What does the aquaporin channel look like on the atomic level? Why does water get through and other things do not?
Use information from seminar and your white boards to draw a second detailed picture of the water channel.
Be sure to capture your drawing as a photograph for your post-lab report!

34. Comparing your Models