1. Animal cell lines

2. CELL LINE
When primary culture is first subcultured or passaged , it became cell line.
ANIMAL CELL CULTURE:
When primary culture of animal cells is first subcultured or passaged , it became animal cell line.

3. THREE TYPES OF CELL LINE
TRANSFORMED CELL LINE
FINITE CELL LINE
CONTINUOUS CELL LINE
Normal cell usually divide only a limited number of times before loosing there ability to proliferate which is a genetically determined event known senescence.
When a finite cell line undergoes transformation and acquires the ability to divide indefinitely .
Conversion of a normal healthy cell into tumorous and cancerous cell.

4. FEATURES FEATURES FINITE CONTINUOUS Ploidy diploid heteroploidy
Anchourage
Dependence yes no
Density limitation yes no
Of growth
Serum requirement high low
Cloning efficiency low high
Growth rate slow (24-96 ) hour high (12-24)hours
Yield low high

5. FINITE CELL LINE CELL LINE ORIGIN SPECIES CHARACTERISTICS
IMR Lung human Susceptible to human viral
infection.
MRC Lung human Susceptible to human viral
Kidney human readily transfected.
B rat melanin
LLC-PKI kidney pig Na+- dependent glucose uptake.

6. CONTINUOUS CELL LINES CELL LINE ORIGIN SPECIES CHARACTERISTICS
Caco Colon Human transport ions and amino acids
Friend spleen Mouse hemoglobin
P388D Mouse grow in suspension
GH1,GH Rat growth hormone
S Mouse Cancer chemotherapy screening

7. TRANSFORMED CELL LINES
CELL LINE ORIGIN SPECIES
3T Mouse
Vero kidney monkey
HeLa epithelial cell human