1. Cardiovascular Physiology: From cardiovascular reactivity studies to endothelial cells culture

2. Goals To present the studies that have been done in the past ten years
To explore next research questions in cardiovascular physiology

3. 2006 : Cardiovascular reactivity in normo-tensive young adults with family history of hypertension

4. Cardiovascular reactivity is a set of changes in blood pressure, heart rate, and other hemodynamic parameters in response to a stimulus (Falkner, 1996).

5. Cardiovascular reactivity
Pre-clinical cardiovascular disease state
Structural and functional changes in cardiovascular system
in clinically healthy individuals that if progress can lead to
manifestations of hypertension, coronary heart failure,
coronary artery disease, and stroke (Treiber et al., 2003).

6. Laboratory methods for assessing cardiovascular reactivity
Mental test
Physical test
Controlled
Short-term
Arithmetic test
Stroop color word conflict test
Video game
Interview
Reaction time
Cold pressor
Postural change
Isotonic exercise
Isometric exercise

7. (Jenie & Noor, 2010)

8. Yogyakarta Earthquake 2006
Cardiovascular reactivity to earthquake (Mutiara Medika, 2006, 9(1), p57-62)
Yogyakarta Earthquake 2006

10. The theme of cardiovascular reactivity as one of topics of research is still going on.
2015 : Cardiovascular reactivity to mental test, cold pressor test, and postural changes in normotensive women who lives in area near the airport compared to normotensive women who lives remote from the airport (The correlation between CV reactivity and noisy)

11. 2009: The role of the renin-angiotensin system in the development atherosclerosis in Wistar rats (an in vivo study on the effect of Captopril to the oxidative stress)
Pathophysiology of atherosclerosis: chronic inflammation, thrombus formation
Role of endothelial cells to inhibit thrombus formation; role of activated platelets

12. Endothelial cells as thromboregulator
(Vinik et al., 2001)

13. Cardiac mortality by gender in the world

14. Umbilical cord collection Isolation of endothelial cells
2010: ROLE OF TESTOSTERONE IN PLATELET ADHESION AND AGGREGATION MEDIATED BY ENDOTHELIAL CELLS : an in vitro Study of cAMP Level in Platelets, Expression of COX-2, ER-Β, ICAM-1, and CD40 in Endothelial Cell Culture Exposed to High Glucose
Umbilical cord collection
Isolation of endothelial cells

15. Primary culture of HUVEC Cell harvest and cell counting
Day 1
Day 3
Day 7

16. Exposure of testosterone and high glucose for 24 hours
Subculture
Exposure of testosterone and high glucose for 24 hours
24 wells microplate
Subculture of HUVEC

17. Hasil dan Pembahasan
Gambar 4.5 Tes agregasi trombosit dimediasi endotel pasca terpapar testosteron dosis bertingkat dalam lingkungan glukosa normal atau glukosa tinggi

18. What next ?

19. Platelets activation: a series of changes in platelets morphology and phenotype, for platelets to become proadhesion, procoagulation, and prothrombosis. 
On the activated platelets, inside-to-outside and outside-to-inside signal occure.

20. 2016: A preliminary study on platelet reactivity in normotensive subjects with a family history of hypertension, CVD-IA proceeding

21. AlhamduLILLAHi Robbil ‘alamiin