1. Turn in your Unit 2 Formative (rubric stapled)
Do Now –
Which two vectors carry plasmid DNA? Naked DNA and liposome
Which two vectors carry ssDNA? Adeno-associated virus and Herpes Simplex Virus
Which vector carries dsDNA? Adenovirus
Which vector carries RNA? Retrovirus
Which vector has a maximum capacity of 5,000 bp? Adeno-associated virus
Take out your packet and Unit 2 Review Guide – turn to the vector table

2. Do Now –
Which two vectors carry plasmid DNA? Naked DNA and liposome
Which two vectors carry ssDNA? Adeno-associated virus and Herpes Simplex Virus
Which vector carries dsDNA? Adenovirus
Which vector carries RNA? Retrovirus
Which vector has a maximum capacity of 5,000 bp? Adeno-associated virus
Take out your packet and Unit 2 Review Guide – turn to the vector table

3. Do Now –
Which two vectors carry plasmid DNA? Naked DNA and liposome
Which two vectors carry ssDNA? Adeno-associated virus and Herpes Simplex Virus
Which vector carries dsDNA? Adenovirus
Which vector carries RNA? Retrovirus
Which vector has a maximum capacity of 5,000 bp? Adeno-associated virus
Take out your packet and Unit 2 Review Guide – turn to the vector table

4. Do Now –
Which two vectors carry plasmid DNA? Naked DNA and liposome
Which two vectors carry ssDNA? Adeno-associated virus and Herpes Simplex Virus
Which vector carries dsDNA? Adenovirus
Which vector carries RNA? Retrovirus
Which vector has a maximum capacity of 5,000 bp? Adeno-associated virus
Take out your packet and Unit 2 Review Guide – turn to the vector table

5. Do Now –
Which two vectors carry plasmid DNA? Naked DNA and liposome
Which two vectors carry ssDNA? Adeno-associated virus and Herpes Simplex Virus
Which vector carries dsDNA? Adenovirus
Which vector carries RNA? Retrovirus
Which vector has a maximum capacity of 5,000 bp? Adeno-associated virus
Take out your packet and Unit 2 Review Guide – turn to the vector table

6. Do Now –
Which two vectors carry plasmid DNA? Naked DNA and liposome
Which two vectors carry ssDNA? Adeno-associated virus and Herpes Simplex Virus
Which vector carries dsDNA? Adenovirus
Which vector carries RNA? Retrovirus
Which vector has a maximum capacity of 5,000 bp? Adeno-associated virus
Take out your packet and Unit 2 Review Guide – turn to the vector table

7. 2.2.1: Gene Therapy

8. Objective
SWBAT choose the appropriate vector for each gene therapy scenario.
SWBAT review for their Unit 2 Test.

9. 2.2.1: Gene Therapy

10. 2.2.1: Gene Therapy

11. 2.2.1: Gene Therapy

12. 2.2.1: Gene Therapy

13. 2.2.1: Gene Therapy

14. 2.2.1: Gene Therapy

15. 2.2.1: Gene Therapy

16. 2.2.1: Gene Therapy

17. 2.2.1: Gene Therapy
Follow the worksheet – complete on looseleaf (determine the appropriate vectors for each condition – then explain which vector is the best)
Answer and discuss the : conc questions.

18. 2.2.1: Gene Therapy

19. 2.2.1: Gene Therapy

20. 2.2.1: Gene Therapy

21. 2.2.1: Gene Therapy

22. 2.2.1: Gene Therapy

23. 2.2.1: Gene Therapy
Diseases caused by single gene mutations that cause defective proteins in somatic cells. The researchers test out the vector before actually trying it out on a patient or a patient’s cell. Cells are infected with the viral vector ex vivo (outside of the body) before an in vivo therapy is tried.
The advantages of using viral vectors for gene therapy include: it can target specific cells, it can integrate into the host’s DNA. The disadvantages: can interrupt genes upon insertion, and it can cause an immune response.

24. 2.2.1: Gene Therapy
Gene therapy is the practice of inserting functional genes into a person’s genome to replace faulty genes. When choosing the appropriate vector you want to be sure the gene of interest will fit into the vector, the vector targets the right cells, and it should limit side effects.
The keys to solving this problem are:
1) the type of cell that is the target of the vector [nerve cell]
2) the characteristics of the target cell [nerve cells do not divide]
3) the length of the gene to be carried by the vector [8,400 bp]
Sounds like Herpes Simplex Virus would be an appropriate vector.

25. 2.2.1: Gene Therapy
Increases strength of athletes’ muscles. Could inject a gene that generates testosterone, growth hormone, or a muscle-strengthening protein.
Opinion.

26. 2.2.1: Gene Therapy
Invasive - would need to isolate DNA, then sequence the DNA to see if the DNA sequence has changed compared to DNA found in the non targeted somatic cells.
There have been many failures along with the successful cases. Many are still violently opposed to the experimentation.
As of 2007, there have been 1,340 completed or approved gene therapy clinical trials in 28 different countries worldwide. The United States is responsible for 64.2% of all gene therapy trials. Other major contributors include the United Kingdom at 11.1%, Germany at 5.5%, and Switzerland at 3.1% (Edelstein et al. 2007).

27. Exit Slip
Have you already started studying for your test? Why or why not?

28. Homework Do Now/Exit Slip Reflection Parent Signature EC Review Guide
Unit 2 Test on Tuesday

29. Video