1. How to extract DNA from anything living

2. Materials: Graduated cylinder Extraction solution Becker
Pounded banana
Strainer
Loop of glass

3. What is inside the extraction solution
Sodium Chloride NaCl
In this experiment, sodium chloride acts as an enzyme to cut proteins just like a pair of scissors.
The DNA in the nucleus of the cell is molded, folded, and protected by proteins.
The sodium chloride cuts the proteins away from the DNA.
Detergent Solution
As you should know the cell has two membranes made of phospholipids (fat).
The detergent attaches the lipid because of their similar structure.
And allow the DNA to float in the solution

4. Step 1: Separating the cells
Put the mashed fruit into a becker
Add 10 ml of extraction solution, composed of sodium chloride and detergent solution
Mix for 5 minutes
Strain the solution to remove the thickest part
Pour the mixture into a test tube

5. Step 2: Alcohol Separation
Tilt your test tube and slowly pour the ethanol into the tube down the side so that it forms a layer on top of the cell mixture.
Pour until you have about the same amount of alcohol in the tube as cell mixture.
Alcohol is less dense than water, so it floats.
As you can see the DNA is already visible here

6. Why did we add alcohol?
DNA normally stays dissolved in water, but when salty DNA comes in contact with alcohol it becomes undissolved. This is called precipitation. The physical force of the DNA clumping together as it precipitates pulls more strands along with it as it rises into the alcohol.
You can use a wooden stick or a straw to collect the DNA. If you want to save your DNA, you can transfer it to a small container filled with alcohol.

7. Step 3: Extraction Extracted DNA
Use the loop of glass to take the DNA out of the test tube. Do it slowly and carefully to not damage the result
Extracted DNA

8. End