1. Precipitation Methods Affinity Chromatography Methods
Source Material
Rabbit sera
Pre-immune
Vaccine induced
Human serum/plasma (natural exposure)
Normal blood bank
Immune, natural exposure
Step 1
Precipitation Methods
Affinity Chromatography Methods
SEP-EASE (PEG method)
Caprylic acid/(NH4)2SO4
Protein G sepharose
Protein A/G sepharose
Step 2
Biochemical Characterization of Igs
Quantification
Concentration of total Ig (NanoDrop)
Concentration of Ag-specific IgG (ELISA titer)
Purity and Integrity
SDS-PAGE
Isotype specific MSP-1p42 ELISA
Affinity ELISA
Step 3
Biological Characterization of Igs (Growth Inhibition Assay)