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Service New Hire Training

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Presentation on theme: "Service New Hire Training"— Presentation transcript:

1 Service New Hire Training
Edition Benjamin D. Bauknecht Esq.

2 Introduction Instructor – Ben Bauknecht
Schooling Associates of Applied science MCCC Specialty in PC Hardware Work 10+ Years in Customer Service 5 years in Hardware Manufacturing/Service 3 years at Essen BioScience

3 Essen BioScience Overview (9:00-11:00)
A privately-owned biotechnology company specializes in developing and manufacturing instruments, software , reagents & consumables that enable real-time live-cell imaging and data analysis for life science researchers. Founded in Ann Arbor, Michigan, USA in 1999 Double-digit organic growth in the past 11 years ~125 employees Subsidiaries in Europe, Japan Headquarter in Ann Arbor, Michigan

4 Milestones 1992 2002 2005 2008 2012 2016 Celebrated the 11th consecutive year of rapid revenue growth Founders Kirk Schroeder and Brad Neagle invented FLIPR (Fluorescence Imaging Plate Reader ) Launched IonWorks, the world’s first truly high throughput electrophysiology system & associated PatchPlate consumable. 1st generation of the IncuCyte™ instrument was born. It was the first automated imaging system designed to fit inside a standard cell culture incubator. Released IncuCyte™ FLR, an upgrade of IncuCyte™ Gen 1 system. Launched 2nd generation of the IncuCyte™ system: the IncuCyte ZOOM®

5 IncuCytes

6 Where is Essen known?

7 What is an IncuCyte?

8 IncuCyte Live-Cell Imaging System
Multiple Imaging Modes HD phase-contrast, green & red fluorescence, 4x 10x 20x Sample area to whole well image capture, up to 6 plates at once 24h scheduling and ‘scan on demand’ Multi-Vessel, Multi-User Compatible with over 300 standard plates, dishes & flasks Mix & match vessels & assays (slides, dishes, flasks, and 6 to 384-well plates) Remote Viewing & Analysis Fully automated, fast on the fly analysis Shared access via any networked Windows device

9 What is an IncuCyte? Advantages:
Multiple users per instrument in parralell Minimal Connections Multi Instrument Access 24/7 Scanning* All-in-one solution Multiple Vessels, Cell Cultures Analyzed at the same time

10 What is Cell Culture? How does the IncuCyte use it?
Removal of cells from an animal or plant and growth in a favorable artificial environment. The cells may be removed from the tissue directly and disaggregated by enzymatic or mechanical means before cultivation, or they may be derived from a cell line or cell strain that has already been already established. Primary Cultures Stable Cell Lines IncuCyte ZOOM was developed to image cells in culture to understand their growth, morphology and behavior. The imaging occurs on the bottom of flasks, microplates, slides and dishes and occurs over long periods of time. The images are analyzed by the software and quantitative data results, allowing scientists to make decisions from their experiments. It enables walk-away automation for multiple vessels and has truly enhanced cell biology research. Data + Decisions!

11 Components of the IncuCyte

12 2 Main Pieces Controller Gantry

13 Controller CPUs RAID Card SSD 3TB HDDs MCU Motor Drivers
In Charge of all Processing Server Computer 2x 6 core proccessors 16GB of RAM (Older units 12GB) 120GB SSD for OS 4x 3TB HDD for storage RAID 5 8.2TB usable space RAID Card 500W Power Supply Additions MCU Board 3x Motor Drivers LCD Touch Screen RAID Card SSD MCU 3TB HDDs Motor Drivers LCD on Front

14 MCU (Motion Control Unit)
Communicates with Motherboard through USB connection Communicates with all sensors in Gantry Through I2C serial interface Controls LCD Touch and Video Controls All Three Motors in Gantry Power Pass-Through to Gantry On Board Firmware Must match FUI version

15 Gantry Optics Assembly X Frame Moving Optical Assembly 3 Motors
X, Y, and Z 2 Circuit Boards Gantry (Breakout) Board Optics Board CCD Camera Certified for Incubator Environment Sensors Temperature X, Y, Z Home Sensors Optics Assembly Gantry Board X Frame

16 Optics Package Lamphouse Objective Filters Condenser Lenses Tube Lens
4x, 10x, 20x Filters Condenser Lenses Tube Lens Fold Mirrors CCD Camera Excitation LEDs Blue Amber

17 Connector to Optics Board
Filter Cube 2 Versions 4459 Dual Color Most Common Blue and Amber Excitation Green and Red Fluorescence Excite Bands nm nm Emission Bands nm nm 4460 No Fluorescence Only Mirrors Connector to Optics Board

18 How does the IncuCyte Work?

19 Setup User Enters a Scan Schedule At Scan Time
Computer gives coordinates of Scan Locations MCU translates Scan Pattern to X,Y,Z Coordinates

20 Transmit LEMO LEMO Cable sends all Commands
Power also goes through LEMO cable LEMO

21 Distribution In Commands, signals, and power sent to Gantry Board
Gantry Board distributes to almost all parts of the IncuCyte Motors LEDs Sensors Optics Assembly Homes Known starting location Moves to Starting X, Y, Z location for first vessel In

22 Distribution (Continued)
Gantry board is Pass-Through for Optics Board Optics Board controls: Camera trigger and power Latch Motor Reads Z Home Sensor Provides Pass-Through for Filter Cube Filter Cube connection on other side

23 At Scan Location (sector/well)
IncuCyte Goes through a WRS (Wide Range Sweep) Locates bottom of Vessel, Bottom of well, and Cell Layer Tall line in image to right Smaller HD Sweep done to collect images in sector Narrowest sweep (Short lines) Next Sector/Well IncuCyte runs Coarse Sweep In between WRS and HD sweep (Smaller line to right of WRS) Fluorescence image is taken at best Focus Plane Only one image taken

24 At Scan Location (Continued)
HD Sweep Collection Collects images to be used by algorithm to create an HD Image IncuCyte repeats HD Sweep for remaining Sectors/Wells If HD Sweep fails, Zoom goes back to coarse sweep to attempt to find correct focus plane Uses Traveling Salesman pattern to move from well to well and vessel to vessel

25 Collection Camera All images sent to controller via Gigabit Ethernet
Computer analyzes images Creates HD Phase Contrast image HD Sweep Image Processing Analyzes smaller sections of images to determine best section out of all images Stitches them together to create a “good” image. Monochrome Saves HD Phase Contrast and Fluorescence image onto D:\ in time coded folders Scan Data saved in same location Camera

26 Additional Processing
Assay User Creates their own experiment on IncuCyte Basic Analyzer ships with all instruments Four purchasable specialized modules Chemotaxis, Scratch Wound, Angiogenesis, and Neurite User teaches the IncuCyte on what to look for in scans Processing Definition Processing Definition can be used for entire length of experiment to gather data Confluence, Cytotoxicity, Migration.

27 Plate Mapping Customer can designate which parts of a plate have which cells IncuCyte remembers Used later to create and display analytics

28 User Interactions All user interactions done through the GUI (Graphical User Interface) GUI must match major version of controller Ex. 2015a Rev 3 Control and 2015a Rev 1 GUI Users Can: View Scans Schedule Scans Analyze Data Backup Data Cannot be analyzed once backed up Administer IncuCyte

29 Accessories WoundMaker 96-well Tool Pipeline
Wounder an entire 96-well plate in no time Necessary for the ScratchWound Module Automated Media Dispenser

30 Consumables http://www. essenbioscience
Plates 96-Well ImageLock Plate Fiducials 24-Well ImageLock Plate Chemotaxis ClearView Plate 3 Layers Reagents Wide range of Reagents and cell lines that are compatible with the IncuCyte

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