1. = Supporting information of
Citrate-modified silver nanoparticles as a colorimetric probe for simultaneous detection of four triptan-family drugs
Sweta K. Laliwala,† Vaibhavkumar N. Mehta,† Jigneshkumar V. Rohit,† and Suresh Kumar Kailasa*
aApplied Chemistry Department, S. V. National Institute of Technology, Surat , India
*Corresponding author, Phone: ; Fax:
†These authors contributed equally to this work.
AgNO3
NaBH4
Trisodium citrate Ag Ag
AgNPs =
Triptan Drugs
Scheme S1. Schematic illustration of the colorimetric detection strategy for triptan drugs based on citrate-capped Ag NPs.

2. Table S1: The structures, molecular weights and solubility behavior of four-triptan family drugs
g/mol
Solubility
pKa values
Rizatriptan
269.4
Water
9.56
Naratriptan
333.46
9.70
Sumatriptan
413.49
9.54
Zolmitriptan
287.35
Methanol
9.55

3. (a)
(b)
(c)
(d)
Figure S1. Effect of time on the UV-visible absorption spectra of citrate-capped Ag NPs after addition of (a) rizatriptan (0.0 min to 2.5 min) (b) naratriptan (0.0 min to 2.5 min) (c) sumatriptan (0.0 min to 3.0 min) and (d) zolmitriptan (0.0 min to 3.5 min).

4. pH
Blank 2 3 4 5 6 7 8 9 10
(a) pH
Blank 2 3 4 5 6 7 8 9 10
(b)
Figure S2. Effect of pH on the generation of UV-visible absorption spectra of induced aggregation of citrate- capped Ag NPs with (a) zolmitriptan (1 mM) and (b) naratriptan (1 mM).

5. pH
Blank 2 3 4 5 6 7 9 10 8
Figure S3. Effect of pH on the SPR peak of citrate-capped Ag NPs without addition of analytes.

6. (a)
aromatic -N-H
stretching
C-H
stretching
aliphatic
-N-H
stretching
-C=C-
stretching
-C-N
stretching
(b)
Figure S4. FT-IR spectra of (a) rizatriptan and (b) citrate-capped Ag NPs with rizatriptan

7. (a)
-C-N
stretching
-C=C-
stretching
aliphatic
-N-H
stretching
aromatic -N-H
stretching
C-H
stretching
-S=O
stretching
(b)
Figure S5. FT-IR spectra of (a) naratriptan and (b) citrate-capped Ag NPs with naratriptan

8. (a)
aromatic -N-H
stretching
-C=C-
stretching
aliphatic
-N-H
stretching
-C-N
stretching
C-H
stretching
-S=O
stretching
(b)
Figure S6. FT-IR spectra of (a) sumatriptan and (b) citrate-capped Ag NPs with sumatriptan

9. (a)
-C=C-
stretching
-C-N
stretching
aromatic -N-H
stretching
aliphatic
-N-H
stretching
-C=O
stretching
C-H
stretching
(b)
Figure S7. FT-IR spectra of (a) zolmitriptan and (b) citrate-capped Ag NPs with zolmitriptan

10. i ii
iii iv v vi
vii
Figure S8. UV-visible absorption spectra for interference study of citrate-capped Ag NPs (i) citrate-capped Ag NPs + different drugs (amoxicillin, ampicilline, cortisone, cyclosporine, methylcobalamine, tramadol) (ii) citrate-capped Ag NPs + triptan drugs (iii) citrate-capped Ag NPs + different drugs + triptan drugs (iv) citrate-capped Ag NPs + different drugs + naratriptan (v) citrate-capped Ag NPs + different drugs + rizatriptan (vi) citrate-capped Ag NPs + different drugs + sumatriptan and (vii) citrate-capped Ag NPs + different drugs + zolmitriptan

11. i ii
iii iv v vi
vii
Figure S9. UV-visible absorption spectra for interference study of citrate-capped Ag NPs (i) citrate-capped Ag NPs (ii) citrate-capped Ag NPs + different drugs (Rabeprazole, Indapamide, Folic acid, Carbidopa, Linsinopril, Afluzosin) (iii) citrate-capped Ag NPs + different drugs + triptan drugs (iv) citrate-capped Ag NPs + different drugs + naratriptan (v) citrate-capped Ag NPs + different drugs + sumatriptan (vi) citrate-capped Ag NPs + different drugs + rizatriptan and (vii) citrate-capped Ag NPs + different drugs + zolmitriptan

12. Figure S10. UV-visible absorption spectra of citrate-capped Ag NPS in the presence of different triptan drugs (naratriptan, sumatriptan, rizatriptan and zolmitriptan)

13. 20 µg/ml
2 µg/ml
Figure S11. UV-visible absorption spectra for different concentrations of rizatriptan in rizatriptan benzoate tablet. Inset picture show the color change of citrate-capped Ag NPs with different concentrations of rizatriptan (20 µg/mL to 2 µg/mL)
20 µg/ml
2 µg/ml
Figure S12. UV-visible absorption spectra for different concentrations of sumatriptan in sumatriptan and naproxen tablet. Inset picture show the color change of citrate-capped Ag NPs with different concentrations of sumatriptan (20 µg/mL to 2 µg/mL)

14. 20 µg/ml
2 µg/ml
Figure S13. UV-visible absorption spectra for different concentrations of zolmitriptan in Zolmist nasal spray. Inset picture show the color change of citrate-capped Ag NPs with different concentrations of zolmitriptan (20 µg/mL to 2 µg/mL)