1. Gene Editing Opportunity for Accelerating Gain
November 2016

2. Genetic Evolution
10,000 BC
2016

3. Efficiency & sustainability
Genetic Evolution
Domestication
Selective breeding
Directed recombination?
1900 AD
2000
2010
2015
10,000 BC
Efficiency & sustainability
prolificacy, feed efficiency, health & robustness
Meat quality
taste, leanness, yield
Darwin develops evolution theory
MU announces PRRS resistant pig
Mendel ‘invents’ genetic science
Gene editing
‘invented’
Shull describes heterosis
Relationship
Based Genomics
Watson & Crick describe DNA molecule
Cost to sequence drops
factor 1000 in 3 years
First transgenic
Crop introduced
Human Genome
Project completed

4. Selective Breeding Works
Prolificacy
14 pigs
Per year
28 pigs
Per year
+100%
Feed conversion
900 lbs of
feed each pig
550 lbs of
feed each pig
-40%
Meat
quality
75 lbs of
lean each pig
120 lbs of
lean each pig
+60%
Manure reduced per lb of lean meat by more than 60%

5. Selective Breeding Evolves
Progressing from traditional BLUP and single
markers to a full description of the genome
along with our global phenotype and
pedigree database – improving the
accuracy of selection
2013
As in F1
RBGS*+
Imputation +
PIC Customized HD/LD genotypes
2014
RBGS*
+ 60K HD
Multiple markers GS
60K HD
2012
SNP Discovery
2010
2009
 2008
*RBGS: Relationship Based Genomic Selection

6. Utilizing the Best Science Relationship Based Genomic Selection
Without genomic data: the “family tree” -
Assume relatedness (r) = 50% for all littermates

7. Utilizing the Best Science Relationship Based Genomic Selection
Genotypes determine what proportion the genome animals share

8. Accelerating Genetic Gain Selective Breeding
PIC Full Program
5 Year Avg
3 Year Avg
1 Year Avg
Index
12.9
15.8
19.4
Pigs weaned/sow/year
0.9
1.1
Pounds weaned/sow/year
11.9
17.4
Pigs marketed/sow/year
Pounds marketed/sow/year
57.2
81.1
126.7
PROFIT PER PIG, $ / pig
2.58
3.16
3.88

9. What’s Next?

10. Accelerating Genetic Gain What’s Next?

11. Accelerating Genetic Gain What’s next?
Genome Sequencing
Impact
Greater selection accuracy
Causal variants
Capture de-novo mutations
Molecular biology targets
Likelihood
High
In-process
Timeline
3 years

12. Accelerating Genetic Gain What’s Next?
Gene Editing

13. The Process

14. The Next Frontier…

15. ...will bring exciting options...

16. ...will bring exciting options...

17. ...and is really already here.

18. Delivering Unique Value

19. PRRS Resistance High Level Overview
Pigs were created with minor nucleotide edits within their existing DNA
No new or foreign DNA was inserted into the pigs

20. What’s next? Conduct trials to validate safety & efficacy
Collaborate with FDA on regulatory framework
Understand and address societal concerns
Look ahead: continue innovation to advance animal ag

21. PIC is dedicated to the responsible exploration of innovations that benefit the well-being of animals, farmers, and society