1. Dairy Research Update
July 2014

2. Scientifically Proven Efficacy

3. Research Proven Performance
Specie
Total Number of Fermentation Metabolites Trials
(1/26/14)
Peer Reviewed
Journal Articles
Dairy
115 41
Beef 49 12
Sheep & Goat 15 4
Swine 52 13
Poultry 48 16
Equine
Pets 9 --
Aquaculture 19 7
In vitro 81 6
Other 3 2
Total
407
105
The Value Of Diamond V Original product Has Been Repeatedly Demonstrated!

5. Research Summary Meta-analysis
Item
DV effect
P-value
Milk Yield, kg/d
1.18
0.001
3.5% FCM, kg/d
1.61
ECM, kg/d
1.65
Milk Fat Yield, g/d 60
0.009
Milk Protein Yield, g/d 30
0.026
DMI (< 70 DIM)
0.62
0.003
DMI (> 70 DIM)
-0.78
0.008
Poppy et al., J. Dairy Sci. 95:

6. Fresh Cow Management

7. Increases DMI in Early Lactation
These transition cows did not increase in intake precalving, but had a nice increase in intake post-freshening. Consistent with IL work.
Robinson et al., J.A.S., 1999

8. Reduces Body Tissue Mobilization During Early Lactation
-0.55
-0.34
-0.50
-0.12
-0.52
-0.39
-0.59
-0.35
-0.29
-0.14
-0.7
-0.6
-0.5
-0.4
-0.3
-0.2
-0.1
0.0
Body Weight Change, kg/d
1irst Calf Heifers
1Mature Cows
2Mature Cows
3Mixed Cows
4Mixed Cows
Control
Original product
This is a summary from four transition cow studies. In all four studies, cows fed XP showed less weight loss compared to controls.
1Robinson and Garrett JAS. 77: ;
2Dann et al., JDS. 83: ;
3Erasmus et al., AFST. 122: ;
4Robinson JDS. 80:
Average daily saving of 0.22 kg/d

9. Diamond V FDA Functional Claim
Helps support dry matter intake (DMI) of dairy cows when fed as part of a total mixed ration during the first 70 days of lactation
Key points: “FDA Reviewed and Accepted”, the term of “FDA approved” applies only to drugs claim.

10. What Does More DMI Mean in Early-Lactation?
More milk
Better reproduction
Healthier rumen
Healthier cows
Greater bottom line

11. Feed Efficiency

12. Eat more and produce much more milk
Early Lactation Cow
Eat more and produce much more milk
Item
Parity
Control
Diamond V
Robinson, 1997
Robinson and Garrett, 1999
Dann et al., 2000
Erasmus et al., 2005
Multiparous
Primiparous
Mixed parity
1.84
1.64
1.72
1.36
1.53
1.90
1.65
1.82
1.49
1.68
Average
Difference (Mcal/kg DM)
Difference 23 kg DMI)
1.62
1.71
0.09
2.07
*Improved energy density of diets in early lactation cows (5.5%)!!!!
*1 Mcal = MJ

13. Eat less and produce more milk
Mid-lactation Cow
Eat less and produce more milk
Item
Control XP
DMI*, kg/d
23.1
22.1
Milk**, kg/d
34.9
35.4
4% FCM, kg/d
31.2
32.0
Feed efficiency
1.38a
1.49b
Production efficiency is especially important with high feed costs. Although neither production nor intake differed significantly between the midlactation cows fed the control or diet containing Diamond V XP, there was a trend for higher production and lower intake in the DV-XP cows. This trend in both areas resulted in a significant improvement in production efficiency (lbs milk / lbs DMI) for cows fed Diamond V XP
a,b P < 0.05
Schingoethe et al., J. Dairy Sci. 87:
*TMR = 1.78 Mcal/kg DM (NEL basis)
**Milk = 0.66 Mcal/kg
1 kg less DMI with 0.5 kg more milk:
2.11 Mcal/d improvement in energy

14. Compromising Lower Energy Diet Eat lower energy diet & produce the same amount of milk
Item
Control XP
P <
DMI, kg/d
28.4
28.0
0.10
NEL intake*, Mcal/d
50.5
48.9
0.05
4% FCM, kg/d
40.5
40.2
0.41
Fat, %
3.49
0.90
Protein, %
3.23
0.98
Fat, kg/d
1.56
1.55
0.25
Protein, kg/d
1.45
1.44
0.95
FCM/DMI
1.43
0.38
FCM/NEL intake, kg/Mcal
0.80
0.82
---
Cows fed XP diet resulted in:
No difference in DMI
Consumed lower energy by design (50.5 vs Mcal/d)
Produced similar amount of FCM
Similar feed efficiency (FCM/DMI) – although cows fed XP diet consumed less energy but produced the same amount of milk.
No changes in body weight (not reported)
*TMR contained 1.9% vs. 1.1% CSFA for Control and XP, respectively.

15. Additional Benefits Other Than Milk
Increases DMI in early lactation
Improves rumen health
Better rumen preparation for the lactation
Alleviating SARA
Mitigating heat stress effect
Better quality milk (fat, protein, SCC)
…..

16. Diamond V Original Product and Ruminal Acidosis

17. DV Original product Reduces Negative Impact of Acidosis
Acidosis problems
 Lactate producers
 Rumen pH
 Ruminal LPS
 Feed efficiency
 Milk fat content
 Milk production
Diamond V solutions
 Lactate utilizers
 Rumen pH
 Ruminal LPS
 Feed efficiency
 Milk fat content
 Milk production

18. Diamond V Original product Fermentation Metabolites Reduces Bacterial Population Shift by SARA
Gram(+)
Gram(-)
Control
Normal
Diamond V
SARA
SARA changed rumen population significantly.
Increase Gram(+)
Decrease Gram(-)
Diamond V Original product maintained healthy normal rumen population during SARA
After we classified the OUTs into different taxonomic level, at phylum level, we got 17 phyla. With no surprise, two most abundant Phyla observed are Firmicutes and bacteroidetes, followed by proteobacteria , sprirochaetes and other 11 phyla.
We observed SARA increased F, and reduce B. However, SCFP changed the population shift only moderately indicating maintain healthy rumen population during SARA challenge.
In general terms the Firmicutes to Bacteroidetes ratio is regarded to be of significant relevance in rumen microbiota composition. It has been reported that the cows fed by a high grain diet have a higher ratio of Firmicute to bacteroidetes, compare to the cows fed by high forage diet. In our study, SARA reduced the abundance of Bacteroidetes while increased that of Firmitues. As a result, the ratio of F/B was higher during SARA. However, for the NoSCFP cows, the increase of F/B ratio is higher that SCFP cows.
Li et al., 2012

19. What Is Lipopolysaccharide (LPS)?
Part of outer membrane of the gram (-) bacteria cell wall
Release when these bacteria lyse
Adapted from J. C. Plaizier 19

20. Rumen pH, LPS and Inflammation
 Ruminal pH
Activation of LPS recognizing receptors (TLR-4 and CD14) ?
 LPS in rumen
Cytokines (e.g., TNFα, IL-1, IL-6)
 LPS in portal blood
Acute phase proteins
(e.g. Hp, SAA and LBP)
 LPS in peripheral blood
Inflammation
Adapted from J. C. Plaizier 20

21. Rumen LPS is associated with
milk fat depression
Zebeli and Ametaj, 2009 JDS

22. Reduces Ruminal Endotoxin Concentration
P = 0.13
The pens were held constant during the study – if a cow got sick she was removed and never returned – no new cows entered the pens during the study.
Li et al., J. Dairy Sci. 95 (Suppl. 1)

23. Maintains Higher Milk Fat Concentration
Li et al., J. Dairy Sci. 95 (Suppl. 1)
Li et al., 2012.

24. Diamond V Original product and Heat Stress

25. Link between Heat Stress and Acidosis
Ruminal pH C O L C O L H O T H O T
pH measured 12 times in 8 hours after feeding.
Cows fed the high forage diet had higher pHs than those on the high concentrate diet, as expected.
When cows were exposed to increased temp and humidity, ruminal pH became more acidic, 0.4° for cows fed the high forage diet and 0.6 ° for cows fed the high concentrate diet. The drop was greater for cows fed the high concentrate diet.
High Forage Diet
High Concentrate Diet
Mishra et al., 1970

26. DV Original product Alleviates Impact of Heat Stress
Heat stress problems
 Rumen microbes
 Rumen pH
 Ruminal LPS
 Milk fat content
 Feed efficiency
 Glucose demand
XP/XPC solutions
 Rumen microbes
 Rumen pH
 Ruminal LPS
 Milk fat content
 Feed efficiency
 Ruminal propionate

27. DV Original product Increases Milk Production During Heat Stress
26.6
27.6
29.9
31.4
28.7
29.7
28.5
30.0
33.8
35.0
36.9
37.6
19.6
22.7
34.4
34.9
39.7
42.0 15 20 25 30 35 40 45
Harris & Lobo,
1988
Harris &
Webb, 1990
Braun, 1993
Nagy et al.,
1996
NC Farm Trial,
1997
Fazenda &
Soares, 1998
Alshaikh et al.,
2002
Schingoethe
et al., 2004
Cooke et al.,
2007
Control
Diamond V Original product
Milk Production, kg/d
Results of 9 Heat Stress Trials

28. DV Original product Increases Milk Fat During Heat Stress
Results of 9 Heat Stress Trials

29. 365 Days Success with Diamond V Original XP/XPC

30. THANK YOU What Questions do you have?
The Trusted Experts in Nutrition & Health™
I would like to thank you for your time. Are there any questions?