1. Euroimmun/Lund University
Meeting
Heiko Herwald
Lund University
Sweden
Lübeck July 7, 2016

3. Sepsis is a complex syndrome
Infecting pathogen
Site of infection
Disease spectrum
Streptococcus pyogenes
Staphylococcus aureus
Escherichia coli
Salmonella spp.
Inf path - >800 diff species?
Site of infection – diiferent outcome

4. “The microorganisms. turn out. to be rather more like bystanders
“The microorganisms ... turn out ... to be rather more like bystanders. It is our response to their presence that makes the disease. Our arsenals for fighting off bacteria are so powerful ... that we are more in danger from them than the invaders.”
Lewis Thomas (1972)

5. Host parasite interactions
Streptococcus pyogenes
Staphylococcus aureus
Escherichia coli
Salmonella spp.

6. Pathogenesis of Severe Sepsis
Infection
Microbial Products
(exotoxin/endotoxin)
Cellular Responses
Platelet
Activation
Coagulation Activation
Kinins
Complement
Cytokines
TNF-, IL-1, IL-6
Oxidases
Coagulopathy/ Disseminated Intravascular Coagulation
Vascular/Organ System Injury
Endothelial damage
Endothelial damage
Multi-Organ Failure
Death

7. cell culture in vivo patient material sample processing RT-PCR mass
spectroscopy
sample processing
BiaCore
Bio-Plex
IVIS
electron
microscopy
microscopy

8. QuantStudio™ 7 Flex Real-Time PCR System
RT-PCR
Real-Time PCR System
broad range of real-time PCR-based applications through
multiplexing capabilities
interchangeable block formats. With a simplified
exceptional reproducibility with minimal well-to-well and instrument-to-instrument variation.

9. SPR biosensor BiaCore T200
label-free system for detailed studies of biomolecular interactions
measurements in real-time
analyze interactants ranging from ions to viruses
small concentrations needed

10. Bio-Plex 200 System Bio-Plex suspension array system
automates the reading of 96-well plates
reliable multiplex assay solution that permits analysis of up to 100 biomolecules in a single sample
yielding up to 9,600 data points in about 35 min

11. In Vivo Imaging Instruments PerkinElmer
IVIS
non-invasive real-time measurements in living animals
2D and 3D quantitative bioluminescence and fluorescence with fast and low dose CT imaging
the system is capable of both luminescence and fluorescence modes of imaging

12. Electron microscopy electron microscopy high resolution
morphological studies
structural studies

13. Microscopy microscopy tissue morphology histology immuno-histology
co-localization

14. cell culture in vivo patient material sample processing RT-PCR mass
spectroscopy
sample processing
BiaCore
Bio-Plex
IVIS
electron
microscopy
microscopy