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A3 First
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People who work in environmental protection
Ecologists Energy managers Environmental managers Environmental consultants Recycling officers Conservation officers
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Environmental protection officers
Monitor industrial sites Check for water pollution Check flood risks Monitor air quality Protect wildlife
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Public laboratories are accredited to check the accuracy and precision of the laboratories results.
They must: Adhere to H&S regulations Train their staff Maintain and check their equipment and instruments
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To check a lab is producing accurate evidence, proficiency tests are carried out.
Identical samples sent to laboratories Samples are analysed Results are compiled Report is sent back to laboratory highlighting if the results differed from other results
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Samples taken are stored
Sterile container to prevent contamination Suitable temperature to avoid deterioration Sealed to prevent contamination or tampering
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Monitoring the effects of climate change
In the oceans: As the oceans warm up Species that live in cold water move northwards towards colder water Species that live in northerly regions become extinct North
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Monitoring pollution in freshwater
Scientists look at indicator organisms Certain fresh-water invertebrates only live in very clean water Some species can live in polluted water What is there shows the level of pollution This is called a biotic index
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Recording visual examination results
Written descriptions (give detail) Sketches or drawings (when accuracy not important) Photographs (accurate details) Video (shows movement)
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Features of an image Sharpness Is image sharp or blurred?
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Features of an image Contrast
Extent to which parts of the image stand out from other objects and the background
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Features of an image Depth of field
Extent to which an object or objects at different distances from camera are in focus. Narrow depth of field
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Features of an image Magnification
Size of image in relation to size of object
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Error in measurements Difference between measured value and true value.
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Types of error Random Values vary unpredictably
Equipment not sensitive or measurements difficult to make Systematic Values vary from true value but in a consistent way Instrument incorrectly calibrated Operator consistently making same errors
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Data collection If everyone uses the same methods to collect data then repeatability and reproducibility are increased.
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Qualitative tests Identification tests Detect presence of substances
E.g. Litmus paper or a pregnancy test
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Quantitative tests Tells you how much of a substance is present
E.g. Indicator solution for pH or a pregnancy test which tells you how many days pregnant you are.
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Assessing water quality
Water can be: Pure Containing dissolved solids Containing suspended solids
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Turbidity Measure of the cloudiness of the water.
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Turbidity is measured with:
Turbidity tube Turbidity meter Secchi disk
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A3 Second
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Environmental protection workers
Ecologists Energy managers Environmental managers Environmental consultants Recycling officers Conservation officers
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Environmental protection officers
Monitor industrial sites Check for pollution – air and water Check flood risks Protect wildlife
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Public laboratories are accredited for accuracy and precision of results.
They Follow H&S Train staff Maintain & check equipment
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To check a labs produce accurate evidence, they do proficiency tests
Identical samples sent to lab Samples analysed Results compiled Report is sent to lab to show if results accurate
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Sample storage Sterile container - prevents contamination
Suitable temp - avoids deterioration Sealed - prevents contamination / tampering
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Monitoring climate change
As the oceans warm up Species move north to colder water Species living in north become extinct North
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Monitoring freshwater pollution
Indicator organisms Some fresh-water invertebrates only live in clean water Some only live in polluted water Used to measure level of pollution - biotic index
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Recording visual results
Written - detail Sketches / drawings – accuracy unimportant Photographs – accuracy important Video – captures movement
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Features of an image
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Sharpness Is image sharp or blurred?
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Contrast Do parts of image stand out from others?
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Depth of field How far away from camera are objects in focus?
Narrow depth of field
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Magnification Size of image in relation to real object
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Error Difference between measurement and true value.
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Types of error Random Values vary unpredictably
Equipment unsensitive / measurements difficult Systematic Values vary but consistently Instrument incorrectly calibrated / operator making same errors
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All use same methods to collect data
Data collection All use same methods to collect data Repeatability & reproducibility increased.
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Qualitative tests Identification tests to detect presence
E.g. Litmus paper (pH) / pregnancy test
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Quantitative tests How much of a substance is present?
E.g. Indicator solution (pH) / pregnancy test which tells you no. of days preg.
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Water quality Pure Contains dissolved or suspended solids
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Turbidity Cloudiness of water
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Turbidity measurement
Turbidity tube Turbidity meter Secchi disk
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A3 Third
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Environmental protection workers
Ecologists Energy and environmental managers Environmental consultants Recycling and conservation officers
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Environmental protection officers
Industrial sites Pollution – air and water Flood risks Wildlife
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Public laboratories accreditation for accuracy and precision
H&S Train staff Maintain & check equipment
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Proficiency tests to check lab accuracy
Identical samples sent Samples analysed Results compiled Report sent
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Sample storage Sterile & sealed container - contamination
Suitable temp - avoids deterioration
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Monitoring climate change
Oceans warm up Species move north – colder Northern species become extinct North
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Freshwater pollution Indicator organisms
Some like clean water, some like polluted. Measurement of pollution level - biotic index
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Recording results Written - detail
Sketches / drawings – accuracy unimportant Photographs – accurate Video –movement
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Features of an image Sharpness – sharp / blurred
Contrast – can you see certain parts? Depth of field – how far from camera in focus? Magnification – how much bigger than reality?
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Sharpness Sharp or blurred?
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Contrast Parts of image stand out?
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Depth of field How far away from camera in focus?
Narrow depth of field
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Magnification Size in relation to reality
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Diff between measurement & reality
Error Diff between measurement & reality
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Types of error Random Vary unpredictably Equipment unsensitive
Systematic Vary predictably Instrument uncalibrated / operator errors
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Repeatability & reproducibility
Data collection All use same methods Repeatability & reproducibility UP
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Qualitative tests Identification tests to detect presence
E.g. Litmus paper (pH) / pregnancy test
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Quantitative tests How much of a substance is present?
E.g. Indicator solution (pH) / pregnancy test which tells you no. of days preg.
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Water quality Pure Dissolved or suspended solids
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Turbidity Cloudiness
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Turbidity measurement
T. tube T. meter Secchi disk
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A3 Fourth and final!!!!
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Environmental protection workers
Ecologists Energy and environmental managers Environmental consultants Recycling and conservation officers
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Environmental protection officers
Industrial sites Pollution – air and water Flood risks Wildlife
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Public laboratories accreditation for accuracy and precision
H&S Train staff Maintain & check equipment
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Proficiency tests to check lab accuracy
Identical samples sent Samples analysed Results compiled Report sent
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Sample storage Sterile & sealed container - contamination
Suitable temp - avoids deterioration
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Monitoring climate change
Oceans warm up Species move north – colder Northern species become extinct North
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Freshwater pollution Indicator organisms
Some like clean water, some like polluted. Measurement of pollution level - biotic index
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Recording results Written - detail
Sketches / drawings – accuracy unimportant Photographs – accurate Video –movement
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Features of an image Sharpness – sharp / blurred
Contrast – can you see certain parts? Depth of field – how far from camera in focus? Magnification – how much bigger than reality?
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Sharpness Sharp or blurred?
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Contrast Parts of image stand out?
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Depth of field How far away from camera in focus?
Narrow depth of field
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Magnification Size in relation to reality
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Diff between measurement & reality
Error Diff between measurement & reality
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Types of error Random Vary unpredictably Equipment unsensitive
Systematic Vary predictably Instrument uncalibrated / operator errors
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Repeatability & reproducibility
Data collection All use same methods Repeatability & reproducibility UP
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Qualitative tests Identification tests to detect presence
E.g. Litmus paper (pH) / pregnancy test
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Quantitative tests How much of a substance is present?
E.g. Indicator solution (pH) / pregnancy test which tells you no. of days preg.
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Water quality Pure Dissolved or suspended solids
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Turbidity Cloudiness
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Turbidity measurement
T. tube T. meter Secchi disk
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A4 Round 1
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Scientific experts need good
Scientific knowledge and understanding Observational Measurement skills Analytical }
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Public analysts need good understanding of
Chemical analysis Law Latest food technology
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Public analysts Check to see if H&S standards are being met
Monitor food safety Check labelling Monitor compliance with legislation Provide research and advice on diseases caused by food
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} Law Enforcement Crime scene investigators (CSIs)
Scenes of crime officers (SOCOs) Record Collect Evidence from crime scenes Package }
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} CSIs and SOCOs Collect fingerprints Examine: victims
suspects for forensic evidence locations }
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Forensic Evidence Hairs Fibres DNA
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Forensic Scientists Analyse information from crime scenes collected by CSIs and SOCOs Do other things such as paternity testing Use electrophoresis
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Colorimetry If a coloured chemical is dissolved in a liquid the intensity of colour of the solution increases as more of the chemical is added.
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Colorimeter Used to measure intensity of colour
Provides quantitative information Allows measurement of concentration of coloured chemical in a solution
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Magnification = eyepiece lens x objective lens
Light microscopes Magnification = eyepiece lens x objective lens Resolution – min. distance required between to points so they can be seen as separate Depth of field – range of distances within which specimen is in focus. Light microscopes have a narrow depth of field so must be focused exactly on object being examined
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Use beam of electrons, not light
Electron microscopes Use beam of electrons, not light Allow much greater: magnification resolution depth of field
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Microscopes Electron vs. light
Expensive Cheap Static Portable Slow Quick Sample must be dead Sample can be living
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Chromatography Technique used to find out what unknown substances are made up of. Thin layer chromatography uses a TLC plate instead of paper. Made of silica gel on glass.
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Chromatography phases
Mobile – solvent used to move substance Stationary – medium substance moves through e.g. Paper Solvent Water if substance dissolves in aqueous solution Alcohol if substance does not dissolve in aqueous solution
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Paper chromatography method
If substance is solid, dissolve in liquid Draw pencil line on chromatography paper Place spot of liquid on paper. Allow to dry Place bottom of paper into solvent Solvent rises up through paper and carries spot with it Chemical in spot separate leaving marks on paper Small molecules travel furthest
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Rf value is how much substance moves in comparison to solvent.
Rf value = Distance travelled by substance Distance travelled by solvent Rf value = small number big number
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Electrophoresis Separates different biological molecules e.g. proteins
Used on small biological samples Helps produce DNA profiles
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Electrophoresis method
Sample placed on absorbent material e.g. gel Charge passed across material Opposites attract -- particles + electrode + particles -- electrode Distance travelled depends on size of molecule and charge. Small molecules with large charge travel furthest.
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Developments in DNA Polymerase chain reaction (PCR) DNA databases
Produces lots of copies of DNA so tiny amounts can be analysed DNA databases Contain profiles of people connected with crimes Stored on national DNA database Allow quick, accurate and reliable comparisons of DNA samples
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A4 Second run through!
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} Scientific experts Scientific knowledge and understanding
Observational Measurement skills Analytical }
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Public analysts Chemical analysis Law Latest food technology
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Public analysts Check to see if H&S standards are being met
Monitor food safety Check labelling Monitor compliance with legislation Provide research and advice on diseases caused by food
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Record, collect and package evidence from crime scenes
Crime scene investigators (CSIs) Scenes of crime officers (SOCOs) Record, collect and package evidence from crime scenes
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CSIs and SOCOs Collect fingerprints and examine victims, suspects& locations for forensic evidence
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Forensic Evidence Hairs Fibres DNA
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Forensic Scientists Analyse crime scene info
Carry out paternity testing Use electrophoresis
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Colorimetry If a coloured chemical is dissolved in a liquid the intensity of colour of the solution increases as more of the chemical is added.
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Colorimeter Measures intensity of colour Provides quantitative info
Determines concentration of coloured chemical in a solution
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Magnification = eyepiece lens x objective lens
Light microscopes Magnification = eyepiece lens x objective lens Resolution – min. distance between points that can be seen as separate Depth of field – range of distances within which specimen is in focus. Light microscopes = narrow depth of field
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Use electrons, not light
Electron microscopes Use electrons, not light Greater: magnification resolution depth of field
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Electron Light Expensive Cheap Static Portable Slow Quick Sample dead
Sample living
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Used to determine what unknown substances are
Chromatography Used to determine what unknown substances are
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Phases Mobile – solvent used to move substance
Stationary – medium substance moves through e.g. Paper Solvent Water Alcohol
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Paper chromatography method
If substance is solid, dissolve in liquid Draw pencil line on chromatography paper Place spot of liquid on paper. Allow to dry Place bottom of paper into solvent Solvent rises up through paper and carries spot with it Chemical in spot separate leaving marks on paper Small molecules travel furthest
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Rf value - how much substance moves in comparison to solvent.
Rf value = Distance travelled by substance Distance travelled by solvent Rf value = small number big number
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Electrophoresis Separates biological molecules e.g. proteins
Used on small biological samples Helps produce DNA profiles
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Electrophoresis method
Sample placed on absorbent material (gel) Charge passed Opposites attract -- particles + electrode Distance travelled depends on charge & size of molecule Small molecules with large charge travel furthest.
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Developments in DNA Polymerase chain reaction (PCR) DNA databases
Produces copy DNA so tiny amount can be analysed DNA databases Profiles of people connected with crimes Stored on national DNA database Quick, accurate and reliable comparisons of DNA samples
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A4 Third run through!
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Scientific experts Scientific K&U Skills in: Observation Measurement
Analysis
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Public analysts Chemical analysis Law Latest food technology
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Public analysts Check H&S standards Monitor food safety
Check labelling Monitor compliance with law Give advice on diseases caused by food
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Crime scene investigators (CSIs) Scenes of crime officers (SOCOs)
Record, collect & package evidence
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Which Forensic Scientists examine
CSIs and SOCOs Check victims, suspects & crime scene for forensic evidence Hairs Fibres DNA Which Forensic Scientists examine
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Colorimetry If coloured chemical is dissolved in liquid intensity of colour of the solution increases as more chemical is added.
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Colorimeter Measures intensity of colour Provides quantitative info
Determines concentration of coloured chemical
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Magnification = eyepiece lens x objective lens
Light microscopes Magnification = eyepiece lens x objective lens Resolution – min. distance between points seen as separate Depth of field – range of distances with specimen is in focus. Light microscopes = narrow depth of field
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Electron microscopes Greater:
magnification resolution depth of field
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Electron Light Expensive Cheap Static Portable Slow Quick Sample dead
Sample living
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Used to determine what unknown substances are
Chromatography Used to determine what unknown substances are
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Phases Mobile – solvent (water or alcohol) Stationary – paper
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Paper chromatography method
If substance is solid, dissolve in liquid Draw pencil line on chromatography paper Place spot of liquid on paper. Allow to dry Place bottom of paper into solvent Solvent rises up through paper and carries spot with it Chemical in spot separate leaving marks on paper Small molecules travel furthest
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Rf value How much substance moves compared to solvent
Rf value = small number big number
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Electrophoresis Separates biological molecules e.g. proteins
Helps produce DNA profiles
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Electrophoresis method
Sample placed gel Charge Opposites attract Small molecules with large charge travel furthest
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Developments in DNA Polymerase chain reaction (PCR) DNA databases
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A4 Last run through!
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Scientific experts Skills in: Observation Measurement Analysis
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Public analysts skills
Chemical analysis Law Latest food technology
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Public analysts do what?
Check H&S standards Monitor food safety Check labelling Monitor compliance with law Give advice on diseases caused by food
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Crime scene investigators (CSIs) Scenes of crime officers (SOCOs)
Record, collect & package evidence
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Which Forensic Scientists examine
CSIs and SOCOs Check victims, suspects & crime scene for forensic evidence Hairs Fibres DNA Which Forensic Scientists examine e.g.
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Colorimetry If coloured chemical is dissolved in liquid intensity of colour of the solution increases as more chemical is added.
153
Colorimeter Measures intensity of colour Provides quantitative info
Determines concentration of coloured chemical
154
Magnification = eyepiece lens x objective lens
Light microscopes Magnification = eyepiece lens x objective lens Resolution – min. distance between points seen as separate Depth of field – range of distances with specimen is in focus. Light microscopes = narrow depth of field
155
Electron microscopes Greater:
magnification resolution depth of field
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Electron Light Expensive Cheap Static Portable Slow Quick Sample dead
Sample living
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Used to determine what unknown substances are
Chromatography Used to determine what unknown substances are
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Phases Mobile – solvent (water or alcohol) Stationary – paper
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Paper chromatography method
If substance is solid, dissolve Draw pencil line paper Spot of liquid. Allow to dry Place paper into solvent Solvent rises and carries spot with it Chemicals separate leaving marks on paper Small molecules travel furthest
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Rf value How much substance moves compared to solvent
Rf value = small number big number
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Electrophoresis Separates biological molecules e.g. proteins
Helps produce DNA profiles
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Electrophoresis method
Sample placed on gel Charge Opposites attract Small molecules with large charge travel furthest
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Developments in DNA Polymerase chain reaction (PCR) DNA databases
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Good luck!!!! Remember: There are no marks for an empty box. If you have time, write something! It is easiest to get the first few marks on a question. Don’t spend ages on one question until you have finished the paper.
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