1. Detection of mycotoxins and mycotoxigenic fungiBy
Abdallah Mahmoud Ahmed Hassane

2. INTRODUCTION

3. Food safety and consumer protection
Food and feed stuffs
Water content
Mycotoxigenic fungi
Mycotoxins

4. MOST COMMON MYCOTOXINS
Fungi
Substrate
Mycotoxin
Aspergillus flavus
Maize, Groundnut,
Cotton seed
Aflatoxins
Aspergillus parasiticus
Aspergillus nomius
Aspergillus ochraceus
Barkey wheat
Ochratoxin
Aspergillus carbonerius
Grapes wine, Coffee
Penicillium verrucosum
Wheat, Barley, Maize
Penicillium expansum
Apple
Patulin
Fusarium oxysporum
Fumonisins
Fusarium sp.
T-2 toxin
Claviceps purpurea
Rye
Ergot alkaloids

6. Secondary metabolites Intermediate Pathway

9. Materials & Methods

10. Determination of moisture content Mycological examination1
Collection of samples 2
Determination of moisture content 3
Mycological examination 4
Detection of mycotoxins in the samples 5
Mycotoxin production ability 6
Molecular Identification of mycotoxigenic fungi

11. 1 2 3 4 5 6 7 8 9 10 11 12 13 14
Collection of samples

13. Mycotoxin production ability
CAM
Amm.
Vapour

14. Mycotoxin production ability

15. Detection of mycotoxins in the samples

16. Bioassay Methods for Mycotoxins
Artemia salina
Bacillus cereus

17. Detection of mycotoxins
Thin layer chromatography (TLC) plates
TLC solvents
Detection

18. Detection of mycotoxins by HPLC

19. Detection of mycotoxins by Dipstick

20. Detection of mycotoxins by ELISA

21. Molecular Identification of mycotoxigenic fungi

22. Control of Mycotoxins
Physical
Chemical
Biological

23. Thank you