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TITLE: A Study on Effect of Shapes on Germination of Fenugreek (Pilot Study) OBJECTIVE: To finding out any effect of Shapes related to its Material and Dimensions on the Germination of Fenugreek
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MATERIALS: 1. Fenugreek Seeds 2. Shapes:
Model Size (cm) Volume (cm3) Wooden Square Pyramid 77 X 77 X 59 2271.5 Fiber Glass Square Pyramid 31.5 X 31.5 X 20 315 Fiber Glass Octagonal Pyramid 11.5 X 11.5 X 19 Ply Wood Square Pyramid 1 Ply Wood Square Pyramid (Russian) 2 32 X 32 X 72.5 1160 3. Room Thermometer 4. Germination Paper 5. Plastic Sheet of 20 cm X 30 cm 6. Digital Camera
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DESIGN: METHODS: Sample Size: 40 seeds placed between germination paper in 4 sets, in each model, each set for the measurement on Day 1, Day 3, Day 5 and Day 7. Total 6 trials has been conducted. Total 240 samples is used for each model.
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Setting: Experiment is conducted by keeping all models in one room at the floor level. 1. Control – keeping sample in open air 2. Wooden Square Pyramid 3. Fiber Glass Square Pyramid 4. Fiber Glass Octagonal Pyramid 5. Ply Wood Square Pyramid 1 6. Ply Wood Square Pyramid (Russian) 2
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Experimental Procedure: 4 sets of 40 samples each is placed between wetted germination paper and rolled and enveloped with plastic sheet and kept in plastic beaker and placed in the each model at the base level.
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On Day 1. one set from each model including of control is taken out, the germination paper is opened and measured germination length, wet weight and dry weight. similarly for the other 3 sets on Day 3, 5 and 7.
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ANALYSIS: 1. Germination Length a
ANALYSIS: 1. Germination Length a. Calculated mean, standard deviation and % of change with respect to control on each set on each trial b. Average of mean and % of change is calculated of all the 6 trials. C. As per the statistical analysis the samples of all the sets of all the models are not normally distributed so calculated significant value by non parametric test 2 Independent Test-Mann-Whitney
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2. Wet and Dry weight. a. Calculated % of change in wet and dry weight with respect to control on each set on each trial b. Calculated average of % of change in wet and dry weight is calculated of all the 6 trials.
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Mean Length and Percent Change on Day 1
Table – 1 Mean Length and Percent Change on Day 1 Sl. Variables Trial 1 Trial 2 Trial 3 Trial 4 Trial 5 Trial 6 Average Ma-Whit Test 1. Control Mean 1.67 1.22 0.93 1.03 2.05 2.53 1.57 2. Wooden Sq 1.42 1.29 1.71 1.32 2.07 1.66 1.58 0.042* % Ch. -15 5.73 83.87 28.16 0.98 -34.39 11.56 3. Fib Gla Sq 1.21 1.53 0.67 1.59 2.15 1.36 0.111 -27.5 25% -27.96 0% -22.4 -11.31 4. Fib Gla Oct 1.2 0.43 0.56 0.71 1.16 1.76 0.97 0.000 -28 64.75 -39.79 -31.06 -43.45 30.43 -17.99 5. Ply Sq 1 (Egyptian) 0.8 1.08 2.09 1.48 0.573 -5 36.8 -13.98 4.85% -18.56 -12.39 -2.21 6. Ply Sq 2 (Russian) 1.3 1.39 0.91 1.6 2.16 1.75 0.334 -22 36 49.46 -11.65 -21.95 -14.62 2.54 Model Size (cm) Volume (cm3) Wooden Square Pyramid 77 X 77 X 59 2271.5 Fiber Glass Square Pyramid 31.5 X 31.5 X 20 315 Fiber Glass Octagonal Pyramid 11.5 X 11.5 X 19 Ply Wood Square Pyramid 1 Ply Wood Square Pyramid (Russian) 2 32 X 32 X 72.5 1160 Model Details
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Mean Length and Percent Change on Day 3
Table – 2 Mean Length and Percent Change on Day 3 Sl. Variables Trial 1 Trial 2 Trial 3 Trial 4 Trial 5 Trial 6 Average Ma-Whit Test 1. Control Mean 6.39 6.72 7.95 5.81 8.36 7.2 2. Wooden Sq 8.96 8.28 5.94 7.39 9.86 8.3 8.12 0.000* % Ch. 40.22 23.21 -25.28 27.19 24.03 -0.71 14.78 3. Fib Gla Sq 6.4 4.25 7.45 4.7 6.91 7.98 6.28 0.001 0.16% -36.76 -62.89 -19.1 -13.01 -4.55 -22.69 4. Fib Gla Oct 0.53 6.66 4.34 7.11 8.2 5.71 15.65 -92.11 -16.27 -41.27 -10.57 -1.91 -24.41 5. Ply Sq 1 6.1 8.54 9.64 6.26 9.71 9.9 -4.54 27.08 21.26 -15.29 22.14 18.42 11.51 6. Ply Sq 2 7.29 8.27 9.03 8.22 9.37 10.03 8.7 14.08 23.07 13.59 11.23 17.86 19.98 16.64 Model Size (cm) Volume (cm3) Wooden Square Pyramid 77 X 77 X 59 2271.5 Fiber Glass Square Pyramid 31.5 X 31.5 X 20 315 Fiber Glass Octagonal Pyramid 11.5 X 11.5 X 19 Ply Wood Square Pyramid 1 Ply Wood Square Pyramid (Russian) 2 32 X 32 X 72.5 1160 Model Details
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Mean Length and Percent Change on Day 5
Table – 3 Mean Length and Percent Change on Day 5 Sl. Variables Trial 1 Trial 2 Trial 3 Trial 4 Trial 5 Trial 6 Average Ma-Whit Test 1. Control Mean 11.37 11.11 11.65 11.53 13.20 13.49 12.06 2. Wooden Sq 13.38 15.46 10.96 12.75 15.36 15.56 13.91 0.000* % Ch. 17.68 39.15 -5.92 10.58 16.36 15.34 15.53 3. Fib Gla Sq 10.33 10.55 12.54 5.49 12.69 13.37 10.83 0.028 -9.15 -5.04 7.64 -52.39 -3.86 -0.89 -10.62 4. Fib Gla Oct 11.96 11.81 12.94 11.55 11.68 16.02 12.66 5.19 6.30 11.07 0.17 -11.52 18.75 5 5. Ply Sq 1 12.63 13.75 10.81 8.52 13.30 15.15 12.36 0.025* 11.08 23.76 -7.21 -26.10 0.76 12.30 2.45 6. Ply Sq 2 13.96 13.44 14.53 14.71 15.98 15.61 22.78 20.97 24.72 27.58 21.06 15.72 22.14 Model Size (cm) Volume (cm3) Wooden Square Pyramid 77 X 77 X 59 2271.5 Fiber Glass Square Pyramid 31.5 X 31.5 X 20 315 Fiber Glass Octagonal Pyramid 11.5 X 11.5 X 19 Ply Wood Square Pyramid 1 Ply Wood Square Pyramid (Russian) 2 32 X 32 X 72.5 1160 Model Details
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Mean Length and Percent Change on Day 7
Table – 4 Mean Length and Percent Change on Day 7 Sl. Variables Trial 1 Trial 2 Trial 3 Trial 4 Trial 5 Trial 6 Average Ma-Whit Test 1. Control Mean 11.24 10.77 11.01 10.97 15.93 15.40 12.55 2. Wooden Sq 13.99 15.61 10.44 9.77 16.73 17.04 13.93 0.000 % Ch. 24.47 44.94 -5.18 -10.94 5.02 10.65 11.49 3. Fib Gla Sq 13.06 10.25 11.59 5.31 11.20 12.72 0.008 16.19 -4.83 5.27 -51.60 -29.69 -17.40 -13.68 4. Fib Gla Oct 18.04 16.28 13.18 10.02 13.15 18.23 14.82 60.50 51.16 19.71 -8.66 -17.45 18.38 20.61 5. Ply Sq 1 15.18 16.74 14.87 10.40 18.20 18.77 15.69 35.05 55.43 35.06 -5.20 14.24 21.88 26.08 6. Ply Sq 2 15.57 15.44 12.25 16.44 15.24 19.56 15.75 38.52 43.36 11.26 49.86 -4.33 27.01 27.61 Model Size (cm) Volume (cm3) Wooden Square Pyramid 77 X 77 X 59 2271.5 Fiber Glass Square Pyramid 31.5 X 31.5 X 20 315 Fiber Glass Octagonal Pyramid 11.5 X 11.5 X 19 Ply Wood Square Pyramid 1 Ply Wood Square Pyramid (Russian) 2 32 X 32 X 72.5 1160 Model Details
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Table – 5 Average of Length (cm) and Percent Change of all 6 Trials on Day 1, 3, 5 & 7 Sl. Variables Day 1 Day 3 Day 5 Day 7 Average Ma-Whit Test 1. Control Mean 1.57 7.2 12.06 12.55 2. Wooden Sq 1.58 0.042* 8.12 0.000* 13.91 13.93 0.000 % Ch. 11.56 14.78 15.53 11.49 3. Fib Gla Sq 1.36 0.111 6.28 0.001 10.83 0.028 10.77 0.008 -11.31 -22.69 -10.62 -13.68 4. Fib Gla Oct 0.97 5.71 12.66 14.82 -17.99 -24.41 5 20.61 5. Ply Sq 1 1.48 0.573 8.36 12.36 0.025* 15.69 -2.21 11.51 2.45 26.08 6. Ply Sq 2 1.75 0.334 8.7 14.71 15.75 2.54 16.64 22.14 27.61 Model Size (cm) Volume (cm3) Wooden Square Pyramid 77 X 77 X 59 2271.5 Fiber Glass Square Pyramid 31.5 X 31.5 X 20 315 Fiber Glass Octagonal Pyramid 11.5 X 11.5 X 19 Ply Wood Square Pyramid 1 Ply Wood Square Pyramid (Russian) 2 32 X 32 X 72.5 1160 Model Details
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Weight (gm) and Percent Change on Day 1
Table – 6 Weight (gm) and Percent Change on Day 1 Sl Variables Trial 1 Trial 2 Trial 3 Trial 4 Trial 5 Trial 6 Average Wet Dry 1 Control Weight 2560 640 2420 670 2390 700 2340 620 2620 660 2610 2490 652 2 Wooden Sq 2540 690 24.75 630 2630 665 2575 650 2542 661 % Ch -2.73 4.96 2.99 6.28 -1.43 5.77 1.61 0.38 0.76 -1.34 4.84 2.22 1.46 3 Fib Gla Sq 25.25 2410 2190 24.15 2520 635 600 2433 634 -1.37 3.13 -0.41 -5.97 -8.37 -8.57 3.21 3.23 -3.82 -3.79 -2.68 -3.23 -2.24 -2.53 4 Fib Gla Oct 2555 6.75 2200 2230 710 2180 610 2500 680 2650 2386 655 -0.20 5.47 -9.10 -7.45 -6.70 1.43 -6.84 -1.61 -4.58 3.03 1.53 2.42 -4.32 0.55 5 Ply Sq 1 2730 2780 750 2345 2260 2660 695 2570 2558 673 6.64 7.81 14.88 11.94 -1.88 -2.86 -3.42 1.50 5.30 -1.53 2.70 3.16 6 Ply Sq 2 2600 2430 2425 2590 675 2539 668 1.56 7.44 -1.49 1.67 3.63 6.45 -1.15 2.27 -0.77 2.06 2.51 Model Details Model Size (cm) Volume (cm3) Wooden Square Pyramid 77 X 77 X 59 2271.5 Fiber Glass Square Pyramid 31.5 X 31.5 X 20 315 Fiber Glass Octagonal Pyramid 11.5 X 11.5 X 19 Ply Wood Square Pyramid 1 Ply Wood Square Pyramid (Russian) 2 32 X 32 X 72.5 1160
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Weight (gm) and Percent Change on Day 3
Table – 7 Weight (gm) and Percent Change on Day 3 Sl Variables Trial 1 Trial 2 Trial 3 Trial 4 Trial 5 Trial 6 Average Wet Dry 1 Control Weight 4330 650 4440 810 4525 780 3640 610 4485 620 4820 1060 4373 755 2 Wooden Sq 4990 820 5125 10.75 4570 775 4390 600 5320 680 4860 1150 4876 850 % Ch 15.24 26.15 15.43 32.72 0.99 -0.64 20.60 -1.64 18.62 9.68 0.83 8.49 11.95 12.46 3 Fib Gla Sq 710 4070 800 4320 760 3725 4465 645 4770 766 5.54 9.23 -8.33 -1.24 -4.53 -2.56 2.34 1.64 -0.45 4.03 -1.04 -1.08 1.85 4 Fib Gla Oct 4760 700 4435 815 4500 715 3820 730 4810 735 4930 1130 4542 804 9.93 7.69 -0.11 -0.62 -0.55 4.95 19.67 7.25 18.55 2.28 6.60 3.96 7.26 5 Ply Sq 1 4510 670 5440 1040 5250 890 5380 5027 855 4.16 3.08 22.52 28.40 16.02 14.10 18.96 9.84 77.06 14.52 11.62 15.06 13.07 6 Ply Sq 2 5420 1380 5365 4710 690 5120 750 5850 1410 5242 959 23.08 22.07 70.37 18.56 -7.05 29.40 13.11 14.16 20.97 21.37 33.02 20.13 25.58 Model Size (cm) Volume (cm3) Wooden Square Pyramid 77 X 77 X 59 2271.5 Fiber Glass Square Pyramid 31.5 X 31.5 X 20 315 Fiber Glass Octagonal Pyramid 11.5 X 11.5 X 19 Ply Wood Square Pyramid 1 Ply Wood Square Pyramid (Russian) 2 32 X 32 X 72.5 1160 Model Details
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Weight (gm) and Percent Change on Day 5
Table – 8 Weight (gm) and Percent Change on Day 5 Sl Variables Trial 1 Trial 2 Trial 3 Trial 4 Trial 5 Trial 6 Average Wet Dry 1. Control Weight 6560 620 6355 715 5840 710 6320 840 6750 1760 6740 1220 6427 977 2. Wooden Sq 6280 610 7525 760 6220 6530 750 7280 1900 7650 2385 6914 1169 % Ch -4.27 -1.61 18.41 6.29 6.51 -14.08 3.32 -10.71 7.85 7.95 13.50 95.49 7.55 13.89 3. Fib Gla Sq 5832 535 5590 685 6670 725 4080 550 6790 1550 7470 1310 6072 893 -11.10 -13.71 -12.04 -4.20 14.21 2.11 -35.44 -34.52 0.59 -11.93 10.83 738 -5.49 -9.15 4. Fib Gla Oct 680 6720 6700 6690 1035 7130 1400 7920 2020 6897 1120 -5.18 9.68 5.74 4.90 14.73 18.31 5.85 23.21 5.63 -20.45 17.51 65.57 7.38 16.87 5. Ply Sq 1 6685 520 7000 570 6255 595 5370 870 7060 1430 7925 2240 6715 1037 19.05 -16.13 10.15 -20.28 7.11 -16.20 -15.03 3.57 4.59 -18.75 17.58 83.61 7.24 2.64 6. Ply Sq 2 7860 650 7420 7675 800 7490 1250 7900 2050 7870 2160 7703 1255 19.82 4.84 16.76 -13.29 31.42 12.68 17.41 48.81 17.04 16.48 16.77 77.05 19.87 24.43 Model Size (cm) Volume (cm3) Wooden Square Pyramid 77 X 77 X 59 2271.5 Fiber Glass Square Pyramid 31.5 X 31.5 X 20 315 Fiber Glass Octagonal Pyramid 11.5 X 11.5 X 19 Ply Wood Square Pyramid 1 Ply Wood Square Pyramid (Russian) 2 32 X 32 X 72.5 1160 Model Details
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Weight (gm) and Percent Change on Day 7
Table – 9 Weight (gm) and Percent Change on Day 7 Sl Variables Trial 1 Trial 2 Trial 3 Trial 4 Trial 5 Trial 6 Average Wet Dry 1. Control Weight 6110 520 6700 550 6630 475 6270 430 7720 540 7390 490 6803 501 2 Wooden Sq 6430 860 8100 870 6070 420 5880 7880 8230 920 7098 668 % Ch 5.23 65.39 20.90 58.18 -8.45 -11.58 -6.22 -2.33 2.07 -3.70 11.37 87.76 4.15 32.29 3 Fib Gla Sq 6865 640 6500 500 6760 530 4010 380 6150 7500 610 6298 515 12.36 23.08 -2.89 -9.09 1.96 11.58 -36.04 -11.63 -20.34 -20.37 1.49 24.49 -7.26 3.01 4 Fib Gla Oct 7850 930 8315 6670 400 5950 5.30 7150 480 8120 910 7343 685 28.48 78.85 24.10 56.36 0.60 -15.79 -5.10 23.26 -7.38 -11.11 9.88 85.71 8.43 36.21 5 Ply Sq 1 7050 840 9200 770 6000 410 8360 8330 810 7843 642 15.39 61.54 37.31 40 22.47 5.26 -4.31 -4.65 8.29 12.72 65.31 15.31 27.29 6 Ply Sq 2 7780 8570 6755 440 7770 450 8010 7909 677 27.33 76.92 27.91 52.72 1.89 -7.37 23.92 4.65 3.76 -7.41 15.97 16.80 36.66 Model Size (cm) Volume (cm3) Wooden Square Pyramid 77 X 77 X 59 2271.5 Fiber Glass Square Pyramid 31.5 X 31.5 X 20 315 Fiber Glass Octagonal Pyramid 11.5 X 11.5 X 19 Ply Wood Square Pyramid 1 Ply Wood Square Pyramid (Russian) 2 32 X 32 X 72.5 1160 Model Details
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Average Weight (gm) and Percent Change of 6 Trials on Day 1, 3, 5 & 7
Table – 10 Average Weight (gm) and Percent Change of 6 Trials on Day 1, 3, 5 & 7 Sl. Variables Day 1 Day 3 Day 5 Day 7 Wet Dry 1. Control Weight 2490 652 4373 755 6427 977 6803 501 2. Wooden Sq 2542 661 4876 850 6914 1169 7098 668 % Ch 2.22 1.46 11.95 12.46 7.55 13.89 4.15 32.29 3. Fib Gla Sq 2433 634 4320 766 6072 893 6298 515 -2.24 -2.53 -1.08 1.85 -5.49 -9.15 -7.26 3.01 4. Fib Gla Oct 2386 655 4542 804 6897 1120 7343 685 -4.32 0.55 3.96 7.26 7.38 16.87 8.43 36.21 5. Ply Sq 1 2558 673 5027 855 6715 1037 7843 642 2.70 3.16 15.06 13.07 7.24 2.64 15.31 27.29 6. Ply Sq 2 2539 5242 959 7703 1255 7909 677 2.06 2.51 20.13 25.58 19.87 24.43 16.80 36.66 Model Size (cm) Volume (cm3) Wooden Square Pyramid 77 X 77 X 59 2271.5 Fiber Glass Square Pyramid 31.5 X 31.5 X 20 315 Fiber Glass Octagonal Pyramid 11.5 X 11.5 X 19 Ply Wood Square Pyramid 1 Ply Wood Square Pyramid (Russian) 2 32 X 32 X 72.5 1160 Model Details
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CONCLUSION: Germination length: On Day 1: significant change in length in wooden pyramid On Day 3: Wooden, Ply Wood 1 and 2 Pyramids has significant change On Day 5: Wooden, Fiber Glass Octagonal, Ply wood 1 and 2 Pyramids has significant change. On Day 7: Wooden, Fiber Glass Octagonal, Ply wood 1 and 2 Pyramids has significant change.
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Weight: On Day 1: Wet weight – Wooden, Ply Wood 1 & 2 Pyramids samples has more weight Dry weight – Wooden, Fiber Glass Octagonal, Ply wood 1 & 2 Pyramid Samples has more weight On Day 3: Wet Weight – Wooden, Fiber Glass Octagonal and Ply Wood 1 & 2 Pyramids has more weight Dry weight – all five model samples has more weight compare to control sample.
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On Day 5: Wet weight – Wooden, Fiber Glass Octagonal, Ply Wood 1 & 2 Pyramids samples has more weight Dry weight – Wooden, Fiber Glass Octagonal, Ply wood 1 & 2 Pyramid Samples has more weight On Day 7: Wet Weight – Wooden, Fiber Glass Octagonal and Ply Wood 1 & 2 Pyramids has more weight Dry weight – all five model samples has more weight compare to control sample.
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